A kind of water-in-oil-in-water emulsifier and its application and application method
A water-in-oil-in-water emulsifier technology, which is applied in pharmaceutical formulations, antiviral agents, medical preparations containing active ingredients, etc., to achieve high commercial value, low price, and easy access
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Embodiment 1
[0039] Embodiment 1 Emulsifier X1
[0040] Emulsifier X1 includes: mineral oil 88 grams; polyethylene glycol 400 monooleate 9 grams; mannitol monooleate 3 grams.
[0041] Its method of use includes the following steps:
[0042] 1) Heat emulsifier X1 to 30°C;
[0043] 2) Take 130 grams of heated emulsifier X and put it into a 200mL beaker, and add another 30 grams of antigen (inactivated porcine foot-and-mouth disease virus) aqueous solution at 35°C to the beaker, and place the beaker under a mechanical stirrer after adding , keep stirring at a speed of 200 rpm for 15 minutes to make the emulsion uniform, and obtain the self-made vaccine emulsion 1.
[0044] The use of the emulsifier X1 is as an adjuvant for porcine foot-and-mouth disease vaccine.
Embodiment 2
[0045] Embodiment 2 Emulsifier X2
[0046] Emulsifier X2 includes: 85 grams of mineral oil; 9 grams of polyethylene glycol 400 monooleate; 3 grams of mannitol monooleate, and 3 grams of fatty alcohol polyoxyethylene ether.
[0047] Its method of use includes the following steps:
[0048] 1) Heat emulsifier X2 to 35°C;
[0049]2) Put 45 grams of heated emulsifier X2 into a 200mL beaker, and add another 30 grams of antigen (inactivated porcine foot-and-mouth disease virus) aqueous solution at 15°C to the beaker, and place the beaker on a mechanical stirrer after adding Keep stirring at a speed of 250 rpm for 15 minutes to make the emulsion uniform, and obtain the self-made vaccine emulsion 2.
[0050] The use of the emulsifier X2 is as an adjuvant for porcine foot-and-mouth disease vaccine.
Embodiment 3
[0051] Embodiment 3 Emulsifier X3
[0052] Emulsifier X3 includes: 82 grams of mineral oil; 9 grams of polyethylene glycol 400 monooleate; 3 grams of mannitol monooleate, and 3 grams of fatty alcohol polyoxyethylene ether.
[0053] Its method of use includes the following steps:
[0054] 1) Heat emulsifier X3 to 30°C;
[0055] 2) Put 40 grams of the heated emulsifier X3 into a 200mL beaker, and add another 30 grams of an aqueous solution of antigen (inactivated porcine foot-and-mouth disease virus) at 20°C to the beaker, and place the beaker on a mechanical stirrer after adding Keep stirring at a speed of 225 rpm for 12 minutes to make the emulsion uniform, and obtain the self-made vaccine emulsion 3.
[0056] The use of the emulsifier X3 is as an adjuvant for porcine foot-and-mouth disease vaccine.
[0057] The emulsifying agent of the present invention includes many kinds, because the raw material of each component and the selection of mass percentage are different, the f...
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Abstract
Description
Claims
Application Information
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