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New Timone massilia strain and application thereof

A technology of Marseilles and new strains, applied to new strains of Marseilles Timones, new strains of Marseilles Timones in the field of oil pollution bioremediation, can solve the problem of secondary pollution by chemical methods, and achieve the ability to reduce the surface tension of liquids , Good biosurfactant ability, good emulsifying activity

Inactive Publication Date: 2016-12-21
CECEP L&T ENVIRONMENTAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the control of oil pollution, the technologies adopted include physical, chemical and biological methods, among which physical methods are to dilute, accumulate or migrate petroleum hydrocarbons to other environments, while chemical methods are difficult to completely degrade petroleum hydrocarbons. And chemical methods will cause secondary pollution

Method used

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  • New Timone massilia strain and application thereof
  • New Timone massilia strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1) Collect the sediment from the dredging boat area of ​​the Xiangjiang River as a sample, and store it in a refrigerator at 4°C. Weigh 30 g of the soil sample to be tested, put it into 100 ml of sterile water, and add 10 g of glass beads. Placed in a constant temperature shaker at 180 rpm and shaken for 30 min.

[0020] 2) After shaking, take out the triangular flask, let stand for 20min at room temperature, take 1ml of supernatant, and carry out a 10-fold serial gradient dilution to 10 under sterile conditions -7 , respectively take 10 -3 , 10 -5 , 10 -7 25 μL of the diluted bacterial suspension of three concentrations was coated on the yeast solid inorganic salt medium coated with toluene in advance, placed in a vacuum desiccator for 24 hours at room temperature, and the plate was inverted when there were no more water droplets on the surface of the plate.

[0021] 3) According to the growth time of the colony on the yeast medium and the difference in the form of...

Embodiment 2

[0028] Morphology and molecular biology research of J42 strain of the present invention.

[0029] The formula of the LB medium used in this example is: yeast extract 5.0g, peptone 10.0g, sodium chloride 10.0g, agar 15.0g, pH 7.2-7.4.

[0030] The J42 strain obtained by the present invention was inoculated on a LB solid medium plate, and was cultured upside down at 30° C. for 24 hours to observe its colony morphology.

[0031] The morphological characteristics of the new strain are: orange-yellow, round and convex, neat edges, smooth and moist surface.

[0032] Bacteria identification

[0033] Take 200 μL of sterile EP tube, add 50 μL of sterile double-distilled water to the tube, then take a small amount of the J42 bacterial strain obtained by the present invention and suspend it in the EP tube, and finally use a pipette to repeatedly suck the liquid in the EP tube to make the bacterial cells. Mix well to make bacterial suspension. The bacterial suspension was treated in a ...

Embodiment 3

[0038] Study on Surfactant Production Characteristics of Strains

[0039] The liquid culture medium formula used in this example is: carbonate buffer 1000mL, MgSO 4 ·7H 2 O0.1g, NH 4Cl 0.5g, Na 2 HPO 4 0.5g, CaCl 2 ·6H 2 O 0.01g, FeSO 4 .7H 2 O0.005g, toluene 5g, trace elements 1mL; the formula of trace elements is: ZnSO 4 ·7H 2 O 0.44g, CuSO 4 ·5H 2 O 0.20g, MnSO 4 ·2H 2 O0.17g, Na 2 MoO 4 ·2H 2 O 0.06g, H 3 BO 3 0.10g, CoCl 2 ·6H 2 O 0.08g, dilute to 1000mL with distilled water; the formula of carbonate buffer is: Na 2 CO 3 0.105g, NaHCO 3 0.756g, dilute to 1000mL with distilled water, pH 8.0.

[0040] Select the new strain B11 of petroleum degrading bacteria, inoculate it into the liquid medium, and control the initial inoculation concentration to be 10 7 cells / mL, cultured in a constant temperature air bath shaker at 30°C and 180rpm for 5 days. Five days later, the bacterial culture solution was drawn to test the biosurfactant production ability. ...

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Abstract

The invention discloses a new strain-Timone massilia J42 and application thereof serving as a petroleum degrading strain in degrading petroleum wastewater. The new strain is obtained by separating from bottom sediment of a Xiang River sand excavating ship area. The strain has high bio-surfactant generating capability, emulsifying activity and liquid surface tension lowering capability. In addition, J42 methylbenzene degrading experiments show that the strain has quite high capability of degrading aromatic hydrocarbon methylbenzene. The strain also presents high COD removing efficiency in petroleum wastewater degrading experiments. The strain has good application prospect in the field of petroleum pollution bioremediation.

Description

technical field [0001] The invention relates to a new strain of Marseilla serratia timone, and also relates to the use of the new strain of Marseilla serratia timone in bioremediation of oil pollution, belonging to the field of biotechnology. Background technique [0002] Oil is a combustible mineral that is formed from the remains of organic matter such as plants or animals during different historical periods of the Earth's formation. Alkanes are the main components of petroleum. With the increase of relative molecular mass, alkanes exist in petroleum in three states: gas, liquid and solid. Aromatic hydrocarbons account for 2-4% of the petroleum content, and there are monocyclic (such as benzene, toluene, xylene), bicyclic (mainly naphthalene), tricyclic aromatic hydrocarbons (such as phenanthrene) and polycyclic hydrocarbons with more than three rings. [0003] The toxicity of petroleum to organisms can be divided into two categories, one is acute poisoning caused by a la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/20
CPCA62D3/02A62D2101/20C02F3/34C12N1/205C12R2001/01
Inventor 李朝晖贺治国潘利祥王龙杨添奇卜庆国罗霖姜朵朵钟慧栗树珍
Owner CECEP L&T ENVIRONMENTAL TECH
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