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Method for detecting external influences of tobacco chewing gum on oral microorganisms of human

A technology for tobacco products and microorganisms, applied in the field of biotechnology applications, can solve problems such as inability to accurately perform biological function tests, difficult dissolution of contents, and inapplicability, and achieve wide applicability, improve accuracy, and improve efficiency. Effect

Inactive Publication Date: 2016-12-14
CHINA TOBACCO YUNNAN IND
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, gum-based tobacco products do not produce smoke and act directly on the oral cavity, so the biological testing methods of traditional cigarettes are not applicable to this product
Snus products in pouches are generally used by sticking them between the gums and lips, and their contents can be dissolved by ordinary soaking methods. Due to the existence of gum base, most of the contents of gum-based tobacco products can be dissolved by direct soaking methods. The content is difficult to dissolve, so that the biological function test cannot be accurately performed

Method used

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  • Method for detecting external influences of tobacco chewing gum on oral microorganisms of human

Examples

Experimental program
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Effect test

Embodiment Construction

[0022] 1. Randomly select a gum-based tobacco product (N-2, nicotine content 2mg / piece), randomly select 2 pieces and put them into a sample tube, and freeze the sample tube in liquid nitrogen for 30 minutes.

[0023] 2. Take out the sample tube from the liquid nitrogen, and then take out the gum-based tobacco product (N-2), immediately pulverize it with a sample pulverizer, and collect the pulverized powder with the sample tube.

[0024] 3. Place the sample tube containing the powdered gum-based tobacco products in liquid nitrogen and freeze for 30 minutes;

[0025] 4. Take out the sample tube from the liquid nitrogen, immediately add 10mL of artificial saliva preheated to 37°C, soak the pulverized gum-based tobacco product powder (N-2), use the extraction conditions of 37°C and 30min, and Stir the extract solution every 2 minutes during the soaking process to make the sample fully contact with the extract solution to facilitate the dissolution of the contents.

[0026] 5. P...

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Abstract

The invention discloses a method for detecting external influences of tobacco chewing gum on oral microorganisms of human. The method comprises the following steps: (1) the tobacco chewing gum to be detected is frozen in liquid nitrogen; (2) the tobacco chewing gum is pulverized by a pulverizer; (3) pulverized tobacco chewing gum is frozen again; (4) the pulverized tobacco chewing gum is immersed in artificial saliva; (5) centrifugation is carried out for an extract; (6) an osmotic pressure tester is used for determination; (7) the content of nicotine in the extract is determined; (8) in vitro culture is carried out for oral microorganisms of human; (9) the extract of the tobacco chewing gum is added into cultivated oral microorganisms of human; (10) growth condition is checked, and colony counting is carried out.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to the determination of the influence of gum-based tobacco products on human oral microorganisms in vitro. Background technique [0002] Gum-based smokeless tobacco product (Tobacco chewing gum) is an oral smokeless tobacco product made by adding a certain amount of tobacco extract, spices and other edible additives to the gum base. Gum-based tobacco products are used by putting them in the mouth and chewing them. The chewing extracts will merge with saliva and gather in the mouth, and be spit out or swallowed according to different consumption habits. Since this product mainly acts on the oral cavity and directly contacts with the oral cavity, it has a direct impact on human oral microorganisms. Therefore, it is necessary to establish a method to detect its in vitro effect on human oral microorganisms in order to evaluate its biological function. [0003] Existing biologic...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCC12Q1/025
Inventor 高茜黄海涛李雪梅倪红梅夭建华曾婉俐米其利管莹唐萍朱洲海
Owner CHINA TOBACCO YUNNAN IND
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