Method for capturing tumor cells by aid of magnetic nano-particles and application of method

Abstract

The invention discloses a method for capturing cells by the aid of magnetic nano-particles. The method includes that the magnetic nano-particles and samples with the circulating tumor cells (CTC) are blended with one another, the circulating tumor cells CTC and the magnetic nano-particles are combined with one another to obtain circulating tumor cells CTC with magnetic nano-particle markers, the samples with the circulating tumor cells CTC with the magnetic nano-particle markers flow through capillary tubes with the sectional inner diameters of 1 mm at the flow rate of 2-10 ml per hour, magnetic fields are applied to the peripheries of the capillary tubes when the samples flow through the capillary tubes, the circulating tumor cells CTC with the magnetic nano-particle markers can be deflected under the effects of the magnetic fields to be attracted by the magnetic fields, but normal cells which are not marked by the magnetic nano-particles in the samples can continue flowing through magnetic field zones to be collected by sample collecting pools; the magnetic fields on the peripheries of the capillary tubes are removed, the circulating tumor cells CTC, which are adsorbed on the walls of the capillary tubes, with the magnetic nano-particle markers are washed down by solution and are collected to obtain the tumor cells. The method has the advantages that the circulating tumor cells can be quickly captured by the aid of the method, and the method is easy and convenient to implement, easy to operate and high in universality.

Description

technical field [0001] The invention belongs to the field of biotechnology detection, and specifically relates to a method for obtaining and separating cells, in particular to a method for capturing cancer cells after marking the cells with magnetic nanoparticles, as well as a capturing device and application. Background technique [0002] Common clinical malignant tumors include lung cancer, gastric cancer, breast cancer, liver cancer, colorectal cancer, etc. The spread of cancer cells is the main cause of cancer death, and one of the main culprits of cancer cell spread is circulating cancer cells. Circulating Tumor Cells (CTC) is a collective term for all types of cancer cells that exist in the circulatory system. Once CTCs enter suitable tissues and organs, they can develop into new metastatic cancer lesions. Therefore, the detection of CTC is of great significance for the early etiological diagnosis, treatment strategy formulation, curative effect and prognosis evaluati...

AI Technical Summary

Problems solved by technology

Although other existing methods also use γ-Fe 2 o 3 Magnetic nanoparticles are used for DNA capture, antibody or specific protein capture, all of which are coated with polystyrene or silanized (coated with silicon) on their surface, so that multiple smaller γ-Fe with superparamagnetic 2 o 3 After the magnetic nanoparticles are physically wrapped, they form relatively large spheres (ranging from 200nm to 2mm) to make up for the problem of insufficient magnetic saturation. However, the acquisition of the target object also requires a chemical elution process in the later stage. chemical damage to cancer cells

[0010] Other methods such as: using digital capture system (such as: 201410818225.8) and microfluidic chip capture system based on graphene oxide (such as: Nat Nano., 2013, 8(10), 735~741), and chip-based culture integration system (Oncotarget., 2014, 75(15), supp., 1~15), etc., are difficult to promote and use in reality due to complex process, cumbersome process and inconvenient application.

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