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Buckwheat protein concentrate with low phenol and low trypsin inhibitor and preparation method thereof

A technique of trypsin inhibition and protein concentration, which is applied in the field of deep processing of buckwheat grains, can solve the problems of affecting protein structure and properties, consumption of large acid-base solution, poor grain protein, etc., achieves high solubility and digestibility, and avoids tight combination Effect

Active Publication Date: 2020-04-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The processing and utilization of grain crops has always been a hot issue in my country's food research. It is of great significance to reduce the bad flavor of grain protein and improve its digestibility with new technical means. Little research has been done on buckwheat protein
[0004] On the other hand, the traditional protein extraction process consumes a large amount of acid-base solution, and the environmental pollution caused by it cannot be ignored
However, acidic or alkaline conditions will affect the structure and properties of some proteins, failing to fully retain the natural components of buckwheat

Method used

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  • Buckwheat protein concentrate with low phenol and low trypsin inhibitor and preparation method thereof
  • Buckwheat protein concentrate with low phenol and low trypsin inhibitor and preparation method thereof
  • Buckwheat protein concentrate with low phenol and low trypsin inhibitor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 40g tartary buckwheat powder and 0.033mol / L NaHCO 3 Mix with 0.086mol / L NaCl salt solution at a material-to-liquid ratio of 1:10 (w / v, mass unit is kg, volume unit is L), stir (300r / min) at room temperature 25°C for 12h, and then centrifuge at 8,000r / min 30min. Pre-activated XAD16HP macroporous resin 50g, wet packing column, using dynamic adsorption, the flow rate is controlled at 1 times the column volume per hour, and the sample solution is collected. Finally, dialyze at 4°C for 48 hours and freeze-dry to obtain buckwheat protein concentrate with low phenol and low trypsin inhibitor.

[0046] The recovery rate of polyphenols in this embodiment is 0.32%, and the protein sample is in the form of pure white powder, which can be quickly dissolved in distilled water with pH 7.0.

Embodiment 2

[0058] 20g tartary buckwheat powder and containing 0.033mol / L NaHCO 3 Mix with 0.086mol / L NaCl salt solution at a material-liquid ratio of 1:10 (w / v, mass unit is kg, volume unit is L), stir (300r / min) at room temperature 20°C for 3h, then 8,000r / min Centrifuge for 30min. Take the supernatant in a 1000mL Erlenmeyer flask, add 50g of activated XAD16N macroporous resin, and place it on a constant temperature shaker at 25°C with a rotation speed of 200rpm for static adsorption for 90min. Finally, it was dialyzed at 4°C for 48 hours and freeze-dried to obtain buckwheat protein with low phenol and low trypsin inhibitor.

[0059] The recovery rate of protein in this embodiment is 65.29%, the mass content of total phenols is 0.43%, and the recovery rate of polyphenols is 0.52%. The protein sample is in the form of pure white powder and can be quickly dissolved in distilled water at pH 7.0.

[0060] The sample was extracted with 70% methanol in an ultrasonic water bath, and after p...

Embodiment 3

[0062] 40g tartary buckwheat powder and 0.033mol / L NaHCO 3Mix with 0.086mol / L NaCl salt solution at a material-to-liquid ratio of 1:10 (w / v, mass unit is kg, volume unit is L), stir (300r / min) at room temperature 25°C for 6h, then 8,000r / min Centrifuge for 30min. Take the supernatant in a 1000mL Erlenmeyer flask, add 50g of activated XAD16N macroporous resin, and place it on a constant temperature shaker at 25°C with a rotation speed of 200rpm for static adsorption for 90min. Then use 10kDa polysulfone membrane ultrafiltration diafiltration, the flow rate is 600r / min, the diafiltration volume is 4 times the volume of the sample solution, the concentrated solution is collected, freeze-dried to obtain low-phenol and low-trypsin inhibitor buckwheat protein.

[0063] The recovery rate of protein in this embodiment is 65.16%, the mass content of total phenols is 0.42%, and the recovery rate of polyphenols is 0.53%. The protein sample is in the form of pure white powder and can be...

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Abstract

The invention discloses buckwheat protein concentrate with the low phenol content and the low trypsin inhibitor content and a preparation method of the buckwheat protein concentrate. The purity of the buckwheat protein concentrate reaches 65% to 85%, the total polyphenol mass content is lower than 0.45%, the purity and the total polyphenol mass content of the buckwheat protein concentrate are both significantly lower than those of buckwheat protein prepared through a general alkali dissolution and acid precipitation method, and the buckwheat protein concentrate has the low trypsin inhibitor content and has the good characteristics of the pure white color and luster and the good solubility and digestibility. The preparation method of the buckwheat protein concentrate comprises the steps that extraction is conducted through a saline solution by taking buckwheat cortex powder as a raw material; supernate is taken after centrifuging is conducted and then adsorbed through macroporous resin; an ultrafiltration percolation technique or dialysis is conducted, freeze drying is conducted, and then the buckwheat protein concentrate with the low phenol content and the low trypsin inhibitor content can be obtained. Accordingly, the technological processes are simple, feasible, nontoxic and harmless, and the new-age production requirements of environmental friendliness and sustainable development are really fulfilled; the protein product can be applied to the fields of food and nutritional and healthy products special for special crowds and the like.

Description

technical field [0001] The invention relates to a protein and a preparation method thereof, in particular to a low-phenol and low-trypsin inhibitor buckwheat concentrated protein and a preparation method thereof, belonging to the technical field of deep processing of buckwheat grains. Background technique [0002] Buckwheat protein has a very high biological value, which is equivalent to 92.3% of skim milk powder and 81.4% of egg, and its protein quality is better than that of rice, wheat and corn. In addition, buckwheat protein contains 19 kinds of amino acids, 8 of which are essential amino acids for the human body. The composition is reasonable and the proportion is appropriate. Among them, the content of lysine, phenylalanine, tryptophan and arginine is high, and it is a nutritionally balanced protein. , can be well utilized by the human body. Therefore, supplementing buckwheat protein can effectively improve "lysine deficiency". It can be mixed with other grains to giv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/12
CPCA23J1/12A23V2002/00A23V2200/32
Inventor 杨晓泉陈雅君陈小威王金梅郭健
Owner SOUTH CHINA UNIV OF TECH
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