Clinical-grade cellular protection liquid as well as preparation method and application thereof

Abstract

The invention relates to clinical-grade cellular protection liquid as well as a preparation method and application thereof. The clinical-grade cellular protection liquid contains a human serum albumin injection, a low-molecular-weight dextran and amino acid injection and an isotonic sodium chloride injection. The preparation method comprises the step of uniformly mixing the human serum albumin injection, the low-molecular-weight dextran and amino acid injection and the isotonic sodium chloride injection. Compared with the prior art, the preparation method has the advantages that a proper amount of the human serum albumin injection and a proper amount of the dextran and amino acid injection are creatively added into the isotonic sodium chloride injection, so that nutrients can be provided for cells, meanwhile, acid-base imbalance caused by cellular metabolism can be regulated, cellular damage caused due to acidosis can be relieved, the cell survival rate can be greatly increased, the cell viability can be maintained, the cell survival time is relatively long, the cells can be directly applied to clinical application, and the application is relatively safe and convenient; and the preparation method is simple, convenient and rapid.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a cell protection liquid and its preparation method and application. Background technique [0002] As an independent living body, cells have strong vitality, proliferation and differentiation capabilities, and functional plasticity. The mechanism of cell therapy to treat diseases is mainly divided into two categories: one is the direct effect of cells, which directly uses its specific biological activity to repair damaged tissues and organs; or plays a specific / non-specific killing effect; Indirect effects, such as secretion of related factors or active molecules to regulate the proliferation and functional activities of the patient's own cells. [0003] With the advancement of society and the vigorous development of science and technology, human beings have higher expectations for the quality of life and life expectancy. It is everyone's dream to have a healthy, happy an...

AI Technical Summary

Problems solved by technology

[0006] At present, most of the cell therapy uses normal saline as the commonly used infusion medium. The cells cultured and expanded in vitro are injected into sodium chloride injection, with or without an appropriate amount of human serum albumin, and stored at 0-10°C. , transported to the destination; although this preservation method is simple, safe and convenient in clinical application, it can only be used for short-term preservation, and the cell viability will drop rapidly if the preservation time is too long, which cannot meet the long-distance use in different places; Add an appropriate amount of potassium chloride, magnesium chloride, sodium acetate and other ingredients to preserve the cells that have been cultured and amplified in vitro. Although this method can preserve the cells for a long time, the cell viability decreases rapidly after more than 48 hours, which cannot meet the needs of different places. Remote use, and not directly for clinical use

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