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Cellulosimicrobium funkei stain SLAQ001 for degrading aflatoxin B1 and application of cellulosimicrobium funkei stain SLAQ001

A technology of cellulosic bacteria and aflatoxin, which is applied to cellulosic bacteria SLAQ001 used for degrading aflatoxin B1 and its application field to achieve the effects of improving utilization, alleviating damage and reducing toxicity

Inactive Publication Date: 2014-04-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the degradation of AFB by Cellulobacter fenckii and its metabolites 1 report

Method used

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  • Cellulosimicrobium funkei stain SLAQ001 for degrading aflatoxin B1 and application of cellulosimicrobium funkei stain SLAQ001
  • Cellulosimicrobium funkei stain SLAQ001 for degrading aflatoxin B1 and application of cellulosimicrobium funkei stain SLAQ001
  • Cellulosimicrobium funkei stain SLAQ001 for degrading aflatoxin B1 and application of cellulosimicrobium funkei stain SLAQ001

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Screening, identification and cultivation of Cellulobacter fennerii SLAQ001

[0015] 1. Screening of bacterial strains

[0016] Take soil samples contaminated by polycyclic aromatic hydrocarbons and add them to 100ml of liquid primary screening medium, and enrich and cultivate for 72 hours at 37°C and 140r / min. After the cultivation, take 0.5ml of culture solution and add 4.5ml of sterilized physiological saline, and serially dilute to make the concentration gradient 10 -1 、10 -2 、10 -3 and 10 -4 . Take 10 each -3 and 10 -4 Add 100 μL of the diluted solution to the solid primary screening medium plate, spread evenly until all the liquid is sucked dry, and place it upside down in a 37°C incubator for cultivation.

[0017] Pick the growing colony, streak and inoculate it in a new solid medium for primary screening, transfer three times to isolate and purify the strain, and take the growing strain after three transfers as the primary screening strain.

...

Embodiment 2

[0029] Embodiment 2: Cellulobacter fennerii SLAQ001 is used for degrading pure product aflatoxin B 1

[0030] After the fermentation was finished, the cellulobacterium fermented liquid was taken, centrifuged at 5000r / min for 15min, and the supernatant was filtered through a 0.22 μm sterile filter to remove residual bacterial cells. Take 1.9mL supernatant filtrate and add 100μL aflatoxin B 1 (10μg / mL), adjust the pH value of the reaction system to 6.5, and react at 35°C for 1h, 2h, 4h, 8h, 12h, 24h, 48h, and 72h respectively. Add 4mL chloroform to the reacted sample, shake vigorously for 10min, let it stand for stratification, take the chloroform layer and add it to a 10mL glass tube, repeat the extraction once, dry the extract with an air pump, add 2mL mobile phase to redissolve the glass tube Condensate in the tube, vortex mixed for 3min, the solution was filtered with a 0.45μm microporous filter, and aflatoxin B was detected by high performance liquid chromatography 1 con...

Embodiment 3

[0034] Example 3: Cellulobacter fennerii SLAQ001 is used to degrade AFB in ducklings 1

[0035] 1-day-old Cherry Valley meat ducks were pre-fed with basic diet for 3 days, randomly selected healthy ducklings with similar body weight, and randomly divided them into 3 groups, with 4 replicates in each group and 5 ducklings in each replicate. The specific groups are as follows: blank control group; AFB 1 Attack group; AFB 1 The challenge+Cylobacter fenschii SLAQ001 group. where AFB 1 The amount of attacking virus is 0.1mg / kg·BW, and the amount of microbacteria SLAQ001 added is 10 mg / kg per day. 8 cfu. Fibrobacillus SLAQ001 was added by gavage, and the gavage was continued for 1 week.

[0036] Test results: AFB 1 The feed-to-weight ratio of ducklings in the challenge group (the amount of feed consumed by raising livestock and poultry for one kilogram of weight gain; feed-to-weight ratio = total amount of feed consumed / total weight gain of livestock and poultry) was signific...

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Abstract

The invention discloses a cellulosimicrobium funkei stain SLAQ001. The cellulosimicrobium funkei stain SLAQ001 is preserved in China Center for Type Culture Collection (CCTCC) and the preservation number is CCTCC NO:M2013564. The invention further discloses an application of the cellulosimicrobium funkei stain SLAQ001 to degrading aflatoxin B1. The cellulosimicrobium funkei stain SLAQ001 has a very good degradation capability on the aflatoxin B1 and can resist a gastrointestinal tract environment of an animal body to express the detoxification effect in the animal body, so that the cellulosimicrobium funkei stain SLAQ001 is suitable for removing the aflatoxin B1 in the production of an animal husbandry. The cellulosimicrobium funkei stain can be used for drenching poultries and is used for reducing the toxin on the poultries by the aflatoxin B1 and remitting the damages, caused by the aflatoxin B1, to livers of the poultries.

Description

technical field [0001] The invention relates to Funkei SLAQ001 (Cellulosimrobium funkei stain SLAQ001) and its ability to degrade aflatoxin B 1 in the application. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by molds during growth and reproduction, and are commonly found in feed and feed ingredients. According to the report of the World Food and Agriculture Organization, about 25% of the world's crops are polluted by mycotoxins every year, causing direct or indirect losses of tens of billions of dollars to the livestock industry. Among the several mycotoxins with serious pollution, aflatoxin is considered to be the most carcinogenic and most common toxin. It widely exists in raw materials such as corn, peanuts, and tree nuts, and is identified as a "class IA dangerous substance" by the World Health Organization. Among the aflatoxins that have been isolated so far, aflatoxin B 1 (AFB 1 ) is the most toxic and has carcinogenic, terato...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/00C12R1/01
Inventor 齐德生孙然然张妮娅刘婕宋文静
Owner HUAZHONG AGRI UNIV
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