A microfluidic biochip for high-precision cell arrangement and detection

Abstract

A microfluidic biochip for high-precision arrangement and detection of cells. It consists of a cover layer and a slide. The cover layer is provided with a liquid addition area, a cell arrangement microchannel, an incident optical fiber, an output optical fiber or a miniature photoelectric chip. Detector, fluid expansion area, cell detection area and liquid discharge area; the fluorescently stained cells enter the microchannel from the liquid addition area, pass through the cell arrangement microchannel, and are arranged in a straight line with high precision, and decelerate through the fluid expansion area. Pass through the cell detection area in turn; the laser light is introduced into the cell detection area by the incident optical fiber, sequentially irradiates the cells to be detected, and excites the fluorescence. Accurate detection of cells, the detected cell sample liquid flows out from the liquid discharge area for inactivation and other treatment; the invention can realize high-precision arrangement of cells and accurate and portable detection, and has important application potential in the fields of biomedicine and food science.

Description

technical field [0001] The invention relates to a microfluidic biochip, in particular to a microfluidic biochip for high-precision arrangement and detection of cells. Background technique [0002] Microfluidic chip, also known as microfluidic chip lab or chip lab, is a technology whose main feature is the manipulation and detection of fluids and particles in a micron-scale space. It is used in biomedicine, analytical chemistry and food It has great application potential in science and other fields. It has the advantages of small sample size, high detection efficiency, low cost of use, easy integration with other technical equipment, good compatibility, and is expected to realize portable detection. It has become one of the current research hotspots. one. For example, the Chinese patent application ZL201310602808.2, titled: A microfluidic biochip for cell enrichment and extraction, which uses a sudden-contraction structure to accelerate fluid and cells through the sudden-con...

AI Technical Summary

Problems solved by technology

Although flow cytometry has been widely used in various fields such as biomedicine and clinical diagnosis, it has the following disadvantages: 1) it is expensive and costly, which limits its application in fields such as biomedicine and clinical diagnosis; 2) the volume Large, not portable, and cannot be conveniently applied to on-site first aid, family medical care, and personal health care; 3) The instrument structure is complex, installation is cumbersome, repair and maintenance costs are high, and later use costs are high; 4) Different samples are Need to be processed in the same detection channel, there is a risk of mutual contamination between different samples, which is not conducive to the accurate detection of samples

In addition, it should be pointed out that the sheath flow is used to arrange the cells in an orderly manner. Under high flow conditions, the cells are subject to relatively strong shearing, which can easily cause structural changes and even be destroyed.

Images