Microfluidic biochip for cell high-precision arrangement and detection

Abstract

The invention relates to a microfluidic biochip for cell high-precision arrangement and detection. The microfluidic biochip is composed of a cover plate layer and a slide glass, wherein a charging area, cell arrangement microchannels, an incident optical fiber, an emergent optical fiber or minitype photoelectric detector, a fluid expanding area, a cell detection area and a liquid discharge area are arranged on the cover plate layer. Cells subjected to fluorescent dyeing enters the microchannels from the charging area, are precisely arranged on one line through the cell arrangement microchannels, are decelerated by the fluid expanding area and sequentially pass through the cell detection area. A laser is introduced into the cell detection area through the incident optical fiber, and is sequentially irradiated on the cells to be detected; fluorescence is excited, and the excited fluorescence is guided out by the emergent optical fiber or guided out by the minitype photoelectric detector through photoelectric conversion; signal analysis is carried out to implement precise detection on the cells; and the detected cell sample solution flows out through the liquid discharge area and is subjected to inactivation and other treatment processes. The microfluidic biochip can implement high-precision arrangement and accurate portable detection on the cells, and has important application potential in the fields of biomedicine, food science and the like.

Description

technical field [0001] The invention relates to a microfluidic biochip, in particular to a microfluidic biochip for high-precision arrangement and detection of cells. Background technique [0002] Microfluidic chip, also known as microfluidic chip lab or chip lab, is a technology whose main feature is the manipulation and detection of fluids and particles in a micron-scale space. It is used in biomedicine, analytical chemistry and food It has great application potential in science and other fields. It has the advantages of small sample size, high detection efficiency, low cost of use, easy integration with other technical equipment, good compatibility, and is expected to realize portable detection. It has become one of the current research hotspots. one. For example, the Chinese patent application ZL201310602808.2, titled: A microfluidic biochip for cell enrichment and extraction, which uses a sudden-contraction structure to accelerate fluid and cells through the sudden-con...

AI Technical Summary

Problems solved by technology

Although flow cytometry has been widely used in various fields such as biomedicine and clinical diagnosis, it has the following disadvantages: 1) it is expensive and costly, which limits its application in fields such as biomedicine and clinical diagnosis; 2) the volume Large, not portable, and cannot be conveniently applied to on-site first aid, family medical care, and personal health care; 3) The instrument structure is complex, installation is cumbersome, repair and maintenance costs are high, and later use costs are high; 4) Different samples are Need to be processed in the same detection channel, there is a risk of mutual contamination between different samples, which is not conducive to the accurate detection of samples

In addition, it should be pointed out that the sheath flow is used to arrange the cells in an orderly manner. Under high flow conditions, the cells are subject to relatively strong shearing, which can easily cause structural changes and even be destroyed.

Images