Preparation method and application for SiO2/artemisinin antibacterial nanoparticles
A technology of antibacterial nanoparticles and artemisinin, which can be used in applications, preservation of meat/fish with chemicals, food science, etc., and can solve problems such as antibacterial activity discounts
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Embodiment 1
[0026] Example 1 SiO 2 / Solubility of Artemisinin Antibacterial Nanoparticles
[0027] 1 Experimental materials
[0028]
[0029] 2 Experimental methods
[0030] ①Add 40.0mL of absolute ethanol, 10.0mL of deionized water and 2.0mL of ammonia water into the reaction flask, heat and stir on a heating type magnetic stirrer for 20min (60°C, 300rmp), then add 2.0mL of TEOS dropwise (guaranteed at All the drops were completed within 2 minutes), and 50 mg of artemisinin crystals were added immediately after the drops were completed, the temperature was maintained at 60°C, and stirring was continued at 300 rpm for 60 minutes.
[0031] ②Put the reaction bottle in an oven at 60°C for 48 hours, dry it in vacuum to evaporate the solvent; collect the prepared powder, grind it, wash it, dry it, grind it, and collect it.
[0032] ③Finally, the plasma is used to assist the treatment, and the treatment conditions are: power 800W, time 2min, SiO can be made 2 / Artemisinin Antibacterial...
Embodiment 2
[0040] Example 2 SiO 2 Determination of antibacterial performance of artemisinin antibacterial nanoparticles
[0041] 1 Experimental materials
[0042] SiO 2 / Artemisinin Antibacterial Nanoparticles - Plasma, SiO 2 / Artemisinin antibacterial nanoparticles, artemisinin crystals Strains: Escherichia coli, Staphylococcus aureus
[0043] 2 Experimental methods
[0044] The plate colony counting method was used to determine SiO with Escherichia coli and Staphylococcus aureus as model strains. 2 / Antibacterial properties of artemisinin antimicrobial nanoparticles.
[0045] Escherichia coli and Staphylococcus aureus were inoculated into the liquid medium, placed in an air shaker and cultured for 24-48 hours under the conditions of 37°C and 150rpm, respectively, to obtain a bacterial suspension in the logarithmic growth phase. Take an appropriate amount of Escherichia coli and Staphylococcus aureus that are saturated in the logarithmic phase of growth and add them to a test t...
Embodiment 3
[0049] Example 3 SiO 2 / Practical application of artemisinin antibacterial nanoparticles in beef
[0050] 1 Experimental materials
[0051] SiO 2 / Artemisinin Antibacterial Nanoparticles - Plasma, SiO 2 / Artemisinin antibacterial nanoparticles, artemisinin crystals Strains: Escherichia coli, Staphylococcus aureus Beef (cut into 25×20×5mm size)
[0052] 2 Experimental methods
[0053] The plate colony counting method was used to determine SiO with Escherichia coli and Staphylococcus aureus as model strains. 2 / Antibacterial properties of artemisinin antimicrobial nanoparticles.
[0054] Escherichia coli and Staphylococcus aureus were inoculated into the liquid medium, placed in an air shaker and cultured for 24-48 hours under the conditions of 37°C and 150rpm, respectively, to obtain a bacterial suspension in the logarithmic growth phase. Take an appropriate amount of Escherichia coli and Staphylococcus aureus, which are saturated in the logarithmic phase of growth, an...
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Abstract
Description
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Application Information
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