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Preparation method of silver-loaded shell powder antibacterial agent

A technology of shell powder and antibacterial agent, which is applied in the field of preparation of silver-loaded shell powder antibacterial agent, can solve problems such as shell pollution, achieve enhanced antibacterial effect, rich resources, and good and sustained antibacterial effect

Inactive Publication Date: 2016-12-07
赵雪芹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Coastal areas are rich in shell resources, but at the same time as a large number of salvage, a lot of discarded shells are left behind, causing shell pollution

Method used

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  • Preparation method of silver-loaded shell powder antibacterial agent
  • Preparation method of silver-loaded shell powder antibacterial agent
  • Preparation method of silver-loaded shell powder antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0065] Simultaneously, in the preparation process of the shell powder of the present invention, the treatment process for the secondary calcination of the shell powder can also be carried out after the synthesis of the silver-loaded shell powder. That is, the respective reaction processes of the final synthetic product include the following:

[0066] 1) Secondary calcination after shell powder sodium borohydride method combined with silver

[0067] 2) Secondary calcination after the shell powder is combined with silver by ultraviolet irradiation

[0068] 3) Secondary sintered shell powder combined with silver by sodium borohydride method

[0069] 4) Secondary sintered shell powder combined with silver by ultraviolet irradiation

[0070] 5) One-time Ar sintering with sodium borohydride method combined with silver and then calcination.

[0071] Although the synthetic product of the present invention can be finally obtained through the above synthetic routes, the best method o...

Embodiment 1

[0072] Embodiment 1 inhibition zone experiment

[0073] Indicator species: Escherichia coli.

[0074] Sterilization preparation: tweezers, Oxford cup (inner diameter 6mm±0.2mm), petri dish, double distilled water, calcium oxide control (AR Tianjin Bodi Chemical Co., Ltd.).

[0075] Required equipment: biochemical incubator (Hitachi Hitachi Co., Ltd.), ultra-clean bench (Thermo Fisher Scientific).

[0076] Bacteria preparation:

[0077] Dilute Escherichia coli strains frozen at minus 20 degrees Celsius at 1:100 in liquid medium, and activate the strains on a 37°C shaker at 220rpm for 12-14h. After activation, the bacterial solution was stored in a refrigerator at 4°C. Save it for later.

[0078] Sample preparation: Take the same mass of each sample and place it in an EP tube. After high-temperature sterilization, add sterilized double-distilled water dropwise according to the concentration ratio, and perform ultrasonic diffusion treatment to make the solid particles more fi...

Embodiment 2

[0106] Embodiment 2 Minimum Inhibitory Concentration Determination

[0107] From the experimental results of Example 1, the antibacterial sample made by the sodium borohydride reduction method has the strongest antibacterial ability, and this type of sample is selected for further experiments:

[0108] Preparation of bacterial suspension: take 10 μL of Escherichia coli activated in the above experiment, and incubate on a shaker at 37°C until the logarithmic growth phase (about 20 minutes).

[0109] Prepare medium:

[0110] The formula of nutrient broth NB medium is as follows: (100ml)

[0111] Peptone 1.0g (BR Hangzhou Best Biotechnology Co., Ltd.)

[0112] Beef extract 0.3g (BR Shanghai Sangon Bioengineering Co., Ltd.)

[0113] NaCl 0.5g (AR Shanghai Shisi Hewei Chemical Co., Ltd.)

[0114] PH 7.2±0.2(25℃)

[0115] Dilute the sample antibacterial agent: Take 13 sterilized test tubes and add 1.6ml of culture medium to the first tube. Add 1ml of culture medium to each of th...

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Abstract

The invention records a preparation method of a silver-loaded shell powder antibacterial agent. By preparing the shell powder, mixing the obtained shell powder with a sodium citrate solution and a silver nitrate solution, and dissolving and reducing it with sodium borohydride, the silver-loaded shell powder is obtained. After centrifugal drying, the silver-loaded shell powder antibacterial agent product is obtained. The method of the invention is simple, the sources of raw materials are wide, and the minimum inhibitory concentration of the obtained antibacterial product reaches 4-8 μg / ml, which shows strong antibacterial effect and has wide application value.

Description

technical field [0001] The invention belongs to the technical field of preparation of antibacterial agents, and in particular relates to a preparation method of silver-loaded shell powder antibacterial agents. Background technique [0002] Health is one of the topics we are most concerned about. As a species with strong adaptability, bacteria are almost everywhere in the areas where human beings live. In recent years, infections caused by bacteria have also emerged in an endless stream. For nearly a century, antibiotics have been widely used in various antibacterial fields. disadvantages. Against the background of various problems caused by the misuse of antibiotics, antibiotics have shown a certain degree of limitation. Therefore, people began to turn their attention to some traditional inorganic antibacterial agents, such as the metal ion antibacterial agent mentioned in this article-nano silver antibacterial agent. [0003] With the development of modern nanotechnology...

Claims

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Application Information

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IPC IPC(8): A01N25/08A01N59/16A01N43/16A01P1/00
CPCA01N25/08A01N43/16A01N59/16A01N2300/00
Inventor 赵雪芹刑杰叶婷张永明杨婷任小元
Owner 赵雪芹
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