Preparation method and uses of brassica oleracea protein powder
A protein powder and broccoli technology, which is applied in the field of preparation of broccoli protein powder to achieve the effects of scientific process flow, good water dispersibility and solubility
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Embodiment 1
[0031] Wash 20kg of whole broccoli, dry it, crush it with a pulverizer, squeeze it and filter it with gauze (300 mesh) to get 9.8L of squeezed juice and filter residue, put the squeezed juice into a pot and heat to 70°C, protein flocculation Float on the upper layer until the lower layer has no green color, and filter to obtain a filter cake (flocculation protein) and filtrate (9.0L). After the temperature of 9.0 L of filtrate was lowered to room temperature, it was concentrated using nanofiltration membrane (DK1812C, GE, USA) to obtain 2.8 L of concentrated solution. The flocculated protein and the concentrate were mixed and homogenized, and then spray-dried (BUCHI, small spray dryer B-290) to obtain 720g broccoli protein powder A, with a protein content of 41%.
Embodiment 2
[0033] Wash 20kg of whole broccoli, dry it, crush it with a grinder, squeeze it and filter it with gauze (300 mesh) to get 10.2L of squeezed juice, put the squeezed juice into a pot and heat it to 120°C, the protein flocculates and floats on the Filter the upper layer until the lower layer has no green color to obtain a filter cake (flocculin) and filtrate (9.2L). After the temperature of 9.2L filtrate was lowered to room temperature, it was concentrated using reverse osmosis membrane (SG1812C, GE, USA) to obtain 2.9L concentrate. The floc protein and the concentrate were mixed and homogenized, and then spray-dried (BUCHI, small spray dryer B-290) to obtain 718g broccoli protein powder B, with a protein content of 38%.
Embodiment 3
[0035] Wash 20kg of whole broccoli, dry it, crush it with a grinder, squeeze it and filter it with gauze (300 mesh) to get 9.5L of squeezed juice, put the squeezed juice into a pot and heat it to 70°C, the protein flocculates and floats on the The upper layer is filtered until the lower layer has no green color to obtain a filter cake (flocculation protein) and a filtrate. The floc protein was mixed with 2.8 L of purified water and homogenized, and then spray-dried (BUCHI, small spray dryer B-290) to obtain 682 g of broccoli protein powder C with a protein content of 52%.
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