Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing pterostilbene biosynthetically using o-methyltransferase

A methyltransferase, biosynthesis technology, used in medicine and pharmacology industry, food field

Active Publication Date: 2020-07-31
CONAGEN INC
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present disclosure solves the technical problem of producing pterostilbene in a cell system such as yeast or bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing pterostilbene biosynthetically using o-methyltransferase
  • Method for producing pterostilbene biosynthetically using o-methyltransferase
  • Method for producing pterostilbene biosynthetically using o-methyltransferase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Construction of bacterial expression vectors

[0054] The PCR product of ROMT was cloned into pETite N-His SUMO Kan vector (Lucigen) according to the manufacturer's manual. The resulting plasmid with the right insert was confirmed by sequencing, Sumo-ROMT, and transformed into BL21(DE3) for heterologous gene expression.

[0055] To construct the 4CL::STS fusion gene, At4CL and VvSTS were fused using a PCR amplification strategy. The stop codon of 4CL was removed and a three amino acid linker (Gly-Ser-Gly) was introduced between the open reading frame of 4CL and the STS. This construction resulted in a 2.87 kb fusion gene construct encoding 4CL, tripeptide linker and STS. The fusion gene 4CL::STS cloned into Gateway entry vector using pCR8 / GW / TOPO TA cloning kit (Invitrogen) was transformed into single-use E. coli cells and then sequenced. The 4CL::STS fusion gene was amplified and cloned via BamHI / HindIII into the multiple cloning site of the pETDuet-1 vector, desi...

Embodiment 2

[0057] Construction of Yeast Expression Vectors

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A biosynthetic method for preparing pterostilbene, comprising expressing 4-coumaric acid in a cell system: Coenzyme A ligase (4CL), expressing stilbene synthase (STS) in a cell system, expressing resveratrol in a cell system Reveritol O‑methyltransferase (ROMT), supplying p-coumaric acid to the cell system, growing the cell system in culture, and producing pterostilbene.

Description

[0001] Cross References to Related Applications [0002] The present disclosure is a PCT patent application entitled Method of Using O-methyltransferase for Biosynthetic Production of Pterostilbene. This application claims priority to US Provisional Patent Application No. 61 / 898899, filed November 1, 2013, which is hereby incorporated by reference in its entirety. technical field [0003] The present disclosure is applicable to the food, medicine and pharmacology industries. The present disclosure generally relates to methods for the biosynthetic production of pterostilbene using O-methyltransferase (ROMT). Background technique [0004] Pterostilbene is a stilbene compound chemically related to resveratrol (resveratrol) and is found in blueberries and grapes. It belongs to a class of phytoalexins, agents produced by plants to fight infection. Based on animal studies, pterostilbene is believed to have anti-cancer, anti-hypercholesterolemic, anti-hypertriglyceridemic prope...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H9/00A01H11/00C12N15/52
CPCC12Y203/01095C12Y602/01012C12N9/1007C12N9/1029C12N9/93C12P7/22A61P25/28A61P31/00A61P35/00A61P3/06
Inventor 穆罕默德·瓦杜德·柏惠亚汪业春余晓丹
Owner CONAGEN INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com