A kind of preparation method of mulberry active peptide
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A technology of protein activity and active peptide, which is applied in the fields of medicine and biochemical industry, can solve the problems of mulberry active peptide preparation difficulties, environmental pollution, etc., and achieve the effects of less impurities, reduced environmental pollution, and high conversion rate
Active Publication Date: 2019-02-15
JIANGSU UNIV OF SCI & TECH
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[0006] Purpose of the invention: In order to overcome the difficulties in the extraction of mulberry protein and the low extraction content of mulberry active peptide in the prior art, the present invention provides a preparation method of mulberry active peptide to effectively solve the problems of mulberry protein extraction and mulberry active peptide. To solve the problem of difficult peptide preparation, expand the source of raw materials for active peptide production, avoid environmental pollution caused by redundant mulberry leaves and mulberry bark, mulberry leaves incinerated or discarded, and achieve greater social and economic benefits
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Embodiment 1
[0031] Embodiment 1. Preparation of treatment agent
[0032] Get 1g of ferromagnetic (Fe3O4) graphene oxide, 50g of 0.5wt% dilute hydrochloric acid and 5mL (equivalent to 5g) of commercially available 30wt.% hydrogen peroxide and mix uniformly to prepare the treatment agent.
Embodiment 2
[0033] Embodiment 2. The preparation method 1 of Morin
[0034] Take fresh mulberry leaves or young mulberry branches, take about 8cm tender fresh stems and leaves or mulberry branch skins from the top, clean them, drain and set aside. When in use, cut into small pieces of 0.5-1.0cm, crush them into small pieces smaller than 0.5cm with a pulverizer, take 5g of powder and add 50g of the above-mentioned treatment agent, put it into a beater for uniform beating, recover magnetic graphene oxide with a magnet, and adjust the pH The value was 4.5, and the precipitate was obtained by centrifugation, dissolved in 50 mL of water, and filtered with 8 layers of gauze to obtain the mulberry solution.
Embodiment 3
[0035] Embodiment 3. The preparation method 2 of Morin
[0036] Take fresh mulberry leaves or young mulberry branches, take about 8cm tender fresh stems and leaves or mulberry branch skins from the top, clean them, drain and set aside. When used, cut into small pieces of 0.5-1.0 cm, and crush them into small pieces smaller than 0.5 cm with a pulverizer. Take 1g of powder and add 2g of treatment agent, put it into a beater and beat evenly, recover the magnetic graphene oxide with a magnet, adjust the pH value to 5.5, centrifuge to obtain a precipitate, add 15mL of water to dissolve, and filter with 8 layers of gauze to obtain a mulberry protein solution.
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Abstract
The invention discloses a preparation method of mulberry active peptide, which comprises the following steps: after washing, drying and crushing mulberry leaves and mulberry bark, adding a treatment agent composed of magnetic graphene oxide, dilute acid and hydrogen peroxide to extract, and using Magnet recovers magnetic graphene oxide, adjusts the pH value to 4.5-5.5, centrifuges to obtain the precipitate, dissolves the precipitate with water and filters, the filtrate contains mulberry protein solution, heats it, cools and adjusts the pH value to 7-8.5, adds protease to keep warm, and puts Ultrasonic-assisted enzymolysis in an ultrasonic field, high-temperature heating to inactivate the enzyme, centrifugation, collecting the supernatant, decolorization, suction filtration, ultrafiltration, dialysis, ion exchange and concentration to obtain the mulberry active peptide. The present invention adopts mulberry leaf and mulberry bark as raw material to prepare active peptide, and the raw material is agricultural waste with low cost, which is of great significance for environmental protection and resource utilization of waste; the method extracts mulberry protein content and conversion efficiency of mulberry active peptide Both are more than the conventional method, and the physical and chemical properties are stable.
Description
technical field [0001] The invention belongs to the fields of medicine and biochemical industry, and in particular relates to a preparation method of mulberry active peptide. Background technique [0002] Peptides are compounds in which two or more amino acids are connected by peptide bonds, which play important physiological roles in the human body and exert physiological functions. Active polypeptides are called active peptides, also known as biologically active peptides or biologically active polypeptides. Active peptide is a general term for more than one thousand peptides (such as soybean peptide, sea cucumber peptide, etc. are one of the active peptides). It plays a key role in human growth and development, metabolism, disease, aging and death. Active peptides are the most important active substances in the human body. It is precisely because of the increase or decrease of its secretion in the body that human beings have a cycle of infancy, childhood, adulthood, old...
Claims
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