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Compound agricultural insecticidal bactericidal composition and application thereof

An insecticidal sterilization and composition technology is applied in the field of compound agricultural insecticidal and sterilization compositions, and can solve the problems of affecting the insecticidal effect of Metarhizium anisopliae pesticides, poor environmental compatibility of chemical fungicides, and strong toxic effect of Metarhizium anisopliae. , to achieve the effect of not easy drug resistance, significant synergy and manpower saving

Active Publication Date: 2016-11-09
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to improve the insecticidal speed of fungal insecticides, reduce costs, and improve the sterilization efficiency of fungicides, if the combination of fungicides and insecticides can greatly save manpower and material resources, and reduce the number of times pesticides are used, chemical fungicides have poor environmental compatibility. , such as carbendazim, mancozeb and thiophanate-methyl have a strong toxic effect on Metarhizium anisopliae, and have poor compatibility with Metarhizium anisopliae pesticides, which affect the insecticidal effect of Metarhizium anisopliae pesticides

Method used

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  • Compound agricultural insecticidal bactericidal composition and application thereof
  • Compound agricultural insecticidal bactericidal composition and application thereof
  • Compound agricultural insecticidal bactericidal composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Metarhizium anisopliae MBJ6 strain was isolated from the School of Life Sciences, Beijing University of Chemical Technology, cultured on PDAY medium (0.5% yeast extract was added to PDA), and the strains were stored at 4°C. Spore powder: connect the bacteria to the slant of the test tube, and culture it in a 25°C incubator for 7 days; then transfer it to a PDAY plate, and culture it at 25°C for 2 weeks, and collect it for later use when the spores are mature. With the concentration of berberine 3.6 μg / mL as a, the spores of Metarhizium anisopliae were washed with 20 mL of sterile water, and the concentration was set as b (100,000 spores / mL). According to the isobolism method, a culture medium was prepared according to Table 1, and each group was connected with a 5mm gray mold cake. The blank control was only inoculated with Botrytis cinerea with a diameter of 5 mm, without adding berberine and Metarhizium anisopliae. After culturing for 96 hours, observe the colony dia...

Embodiment 2

[0027] Preparation of 13% (Metarrhizia + Berberine) wettable powder

[0028] The weight proportion of each raw material is as follows:

[0029] raw material

[0030] Each raw material is weighed according to weight ratio, metarhizium anisopliae 100g, berberine 30g, licorice polysaccharide 30g, bentonite 620g, pull open powder 100g, sodium dodecylbenzene sulfonate 120g. The preparation process of the above fungicide wettable powder is as follows: Accurately weigh Metarhizium anisopliae, absorb the weighed Metarhizium anisopliae with carrier bentonite, mix evenly, and then add the weighed berberine, licorice polysaccharide, dodecylbenzene Sodium sulfonate and pull open powder were added, mixed evenly, pulverized by a superfine pulverizer for 30 minutes, and passed through a 300-mesh sieve to obtain a 13% (Metarrhizia + berberine) wettable powder.

Embodiment 3

[0032] Preparation of 20% (Metarrhizia + Berberine) wettable powder

[0033] The weight proportion of each raw material is as follows:

[0034] raw material

[0035] The raw materials were weighed according to weight ratio, 100g of Metarhizium anisopliae, 100g of berberine, 100g of rhubarb polysaccharide, 110g of sodium lignosulfonate, 100g of sodium dodecylbenzenesulfonate, and 490g of bentonite. The preparation process of the above fungicide wettable powder is as follows: Accurately weigh Metarhizium anisopliae, absorb the weighed Metarhizium anisopliae with carrier attapulgite, mix evenly, and then add the weighed berberine, rhubarb polysaccharide, dodecane Calcium phenylbenzenesulfonate and calcium lignin xanthate were added, mixed uniformly, pulverized by an ultrafine pulverizer for 30 minutes, and passed through a 300-mesh sieve to obtain a 20% (Metarhizium anisopliae + berberine) wettable powder.

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Abstract

The invention discloses a compound agricultural insecticidal bactericidal composition and application thereof. The compound agricultural insecticidal bactericidal composition contains anisopliae, berberine, a carrier and auxiliary in weight ratio of (2-20):(3-20):(30-70):(20-60), wherein the anisopliae is anisopliae conidium, and the berberine is monomer or mixture containing berberine. The anisopliae and berberine are compounded and used for agricultural insecticide and bactericide and can be used for preventing and treating diseases and insect pests of crops, labour cost of separate use is reduced, water and electricity are saved, environment friendliness is achieved, insecticidal spectrum and bactericidal spectrum of biological pesticide can be expanded, and pesticide applying frequency is reduced. The compound agricultural insecticidal bactericidal composition does not contain chemical pesticide ingredient and is easy to degrade in the environment and safe to human, livestock and the environment, and pathogenic bacteria hardly produce resistance to the composition; and the composition has the advantages of stability, high efficiency and low cost.

Description

technical field [0001] The invention relates to a compound agricultural insecticidal and bactericidal composition and its application, belonging to the technical field of pesticides. Background technique [0002] Metarhizium spp. is an important class of entomopathogenic fungi. The preparations with it as the main biological active ingredient have the characteristics of no residue, easy production, long-lasting effect, strong pathogenicity, good application effect, and non-target Biosafety and other advantages have broad application prospects. It has a parasitic and lethal effect on a variety of agricultural and forestry pests, and has attracted more and more attention as a class of biocontrol microorganisms. Since the 1960s, more than 50 preparations have been developed and registered. However, metarhizium anisopliae insecticides have the disadvantages of single control, slow insecticidal speed and relatively high cost, which are limited in application. [0003] Berberin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/90A01P7/04A01P3/00
CPCA01N43/90A01N63/30A01N2300/00
Inventor 葛喜珍薛科科李映田平芳张元
Owner BEIJING UNION UNIVERSITY
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