A kind of preparation method of Panda Hai scanning electron microscope sample

Abstract

The invention discloses a preparation method of boat-fruited sterculia seed scanning electron microscope samples. The preparation method comprises the following steps that (1) a boat-fruited sterculia seed is subjected to half longitudinal cutting; (2) the boat-fruited sterculia seed subjected to the half longitudinal cutting is dried for 72 to 168h at 37 to 65 DEG C; (3) the boat-fruited sterculia seed obtained in the second step is subjected to seed coat and seed leaf separation; (4) the sample obtained in the third step is put into a fumigating and dying device for osmic acid fumigating and dying; (5) the fumigated and dyed sample is ventilated for 15 to 30minutes in a ventilation cabinet, so that osmic acid remained on the sample surface is volatilized; (6) the sample obtained in the step 5 is put into a vacuum drier to be dried for 2 to 4h; (7) tweezers are used for slightly clamping the side surface of the sample; the observing surface is ensured to be upward; the samples are firmly stuck on a metal sample table by conductive glue in sequence; the metal sample table is put into an ion sputtering instrument for metal spraying film coating and scanning electron microscope observation diagram collection. The preparation method has the beneficial effects that the complete appearance form of the boat-fruited sterculia seed is maintained in the prepared sample; during scanning electron microscope observation, the tissue structure is clear; the image three-dimensional effect is high; efficiency, simplicity and convenience are realized, and the like.

Description

technical field [0001] The invention relates to a preparation method of a scanning electron microscope sample, in particular to a preparation method of a fat sea scanning electron microscope sample. Background technique [0002] The dried and mature seeds of the sycamore plant, Panghaihai, have the functions of clearing away heat and moistening the lungs, relieving throat and detoxification, moistening the intestines and laxative, etc. It is a traditional Chinese medicinal material for both medicine and food. Because of its strong water-swelling properties, it has also attracted attention in the fields of pharmaceutical research such as osmotic activity and propellants in recent years. Through the analysis and research on the ultrastructure of the fat sea, it is of great significance to deeply explore its role in biomedical research. [0003] Due to the strong water-absorbing and swelling properties of Panda Dahai, conventional scanning electron microscope sample preparatio...

AI Technical Summary

Problems solved by technology

Osmic acid has a strong electronic staining effect, and the image of samples fixed by osmic acid has better contrast. The laryngeal mucous membrane has a strong stimulating effect, which is easy to cause pollution and damage to the human body and the environment

[0006] Due to the unique water-swelling characteristics of Panda Dahai, a large amount of glutaraldehyde and osmic acid fixatives need to be consumed in the routine sample preparation process, and glutaraldehyde and osmic acid are volatile and toxic reagents, which not only greatly increase the experimental cost, but also It is easy to cause greater pollution to operators and the environment

[0007] At present, the research technology on the microstructure of Panda Dahai is still immature, and it is still difficult to obtain a sample with complete structure of Panda Dahai by conventional scanning electron microscope sample preparation technology. There is a problem of damage to the structure of the fat sea, and there is a problem of unclear images when observing samples that are not fixed and stained

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