CTL epitope peptide on HTNV GP as well as screening method and application thereof
An epitope peptide, amino acid technology, applied in the field of microbial immunity, can solve the problem that there is no CTL epitope research, report, etc.
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[0030] The following describes the present invention in detail with reference to the drawings and specific embodiments.
[0031] 1. Screening method of CTL epitope peptide on Hantaan virus envelope glycoprotein
[0032] Step1: Forecast
[0033] The amino acid sequence of the glycoprotein of HTNV 76-118 strain was obtained from the NCBI database, and these amino acid sequences were input into the epitope prediction software IMMUNE EPITOPE DATABASE AND ANALYSIS RESOURCE using bioinformatics technology to predict the CTL epitope peptide composed of 8 amino acids, H -2b limit.
[0034] The prediction software predicts a total of 15 epitope peptides on HTNV GP. The amino acids of these 15 epitope peptides are:
[0035] (1) GP aa39~46: SVIGYVEL Serine-Valine-Isoleucine-Glycine-Tyrosine-Valine-Glutamic acid-Leucine;
[0036] (2) GP aa44~51: VELPPVPL valine-glutamic acid-leucine-proline-proline-valine-proline-leucine;
[0037] (3) GP aa64~71: SMDNHQSL serine-methionine-aspartic acid-asparagine-g...
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