Method for removing sulfate and Zn(II) wastewater by virtue of synergism of spongy iron and microorganisms
A sponge iron and sulfate technology, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of low unit microbial content, poor settling performance, strain loss, etc. To achieve the effect of simple equipment, convenient operation and strong electrochemical enrichment
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Embodiment 1
[0038] A method for the collaborative removal of sulfate and Zn(II) wastewater by sponge iron and microorganisms comprises the following steps:
[0039] (1) Preparation of sponge iron
[0040] Under the conditions of carbon content (mass ratio of simple carbon powder to iron slime) 2:3, reaction temperature 1200°C, and reaction time 30 minutes, iron slime was used as raw material to calcinate to prepare conventional sponge iron solid. Activate with dilute hydrochloric acid to prepare a sponge iron solution with a concentration of 0.3 g / L, which is designated as reaction solution A.
[0041] (2) Preparation of sulfate reducing bacteria SRB
[0042] Select 2 rings from a sulfate-reducing bacterium—Desulfovibrio (Desulfovibrio; Desulfovibrio subsp. Transfer to 40ml Desulfovibrio nutrient medium, culture in the dark at 35°C for 5 days, use Desulfovibrio proliferation medium with 5% inoculum to carry out expanded culture for 2 days, and centrifuge at 3000r / min for 20min to obtain...
Embodiment 2
[0054] A method for the collaborative removal of sulfate and Zn(II) wastewater by sponge iron and microorganisms comprises the following steps:
[0055] (1) Preparation of sponge iron
[0056] Under the conditions of carbon content (mass ratio of simple carbon powder to iron slime) 2:3, reaction temperature 1200°C, and reaction time 30 minutes, iron slime was used as raw material to calcinate to prepare conventional sponge iron solid. Activate with dilute hydrochloric acid to prepare a sponge iron solution with a concentration of 0.5 g / L, which is designated as reaction solution A.
[0057] (2) Preparation of sulfate reducing bacteria SRB
[0058] Select 2 rings from a kind of sulfate-reducing bacterium—Desulfovibrio (same as Example 1) purchased by China Common Microorganism Culture Collection and Management Center, transfer it to 30ml nutrient solution, and cultivate it in the dark at 35°C for 4d, and then 5% of the inoculum was expanded with proliferation medium for 3 day...
Embodiment 3
[0070] A method for the collaborative removal of sulfate and Zn(II) wastewater by sponge iron and microorganisms comprises the following steps:
[0071] (1) Preparation of sponge iron
[0072] Under the conditions of carbon content (mass ratio of simple carbon powder to iron slime) 2:3, reaction temperature 1200°C, and reaction time 30 minutes, iron slime was used as raw material to calcinate to prepare conventional sponge iron solid. Activate with dilute hydrochloric acid to prepare a sponge iron solution with a concentration of 0.1 g / L, which is designated as reaction solution A.
[0073] (2) Preparation of sulfate reducing bacteria SRB
[0074] Select 2 rings from a kind of sulfate-reducing bacterium—Desulfovibrio (same as Example 1) purchased by China Common Microorganism Culture Collection and Management Center, transfer it to 30ml nutrient solution, and cultivate it in the dark at 35°C for 5d, 5% of the inoculum was expanded with proliferation medium for 2 days, and ce...
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