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Method for extracting erythrothioneine from domestic fungi by using ultrasonic-microwave assisted combined vacuum freeze drying

A vacuum freeze-drying and microwave-assisted technology, applied in organic chemistry and other fields, can solve the problems of low impurity removal rate, low extraction rate, high energy consumption, etc., and achieve the effect of high extraction rate, simple process and short time consumption

Active Publication Date: 2016-09-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The extraction method of reported ergothioneine has the problems of long time, low extraction rate, low impurity removal rate and high energy consumption.
Simply using microwave or ultrasonic extraction cannot fully dissolve ergothioneine from the material

Method used

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  • Method for extracting erythrothioneine from domestic fungi by using ultrasonic-microwave assisted combined vacuum freeze drying
  • Method for extracting erythrothioneine from domestic fungi by using ultrasonic-microwave assisted combined vacuum freeze drying
  • Method for extracting erythrothioneine from domestic fungi by using ultrasonic-microwave assisted combined vacuum freeze drying

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: vacuum freeze-dried Pleurotus eryngii

[0034] Cut fresh and clean Pleurotus eryngii crosswise into thin slices with a thickness of 0.5cm, freeze-dry at -70°C, and then vacuum-dry for 24 hours; crush the dried Pleurotus eryngii and pass through a 70-mesh sieve to obtain dry powder of Pleurotus eryngii, -70 ℃ sealed storage.

Embodiment 2

[0036] The dried powder of Pleurotus eryngii obtained in Example 1 is added according to the ratio of material to liquid 1:20, 1:30, 1:40, 1:50, and 1:60 of ethanol with a volume percentage concentration of 70%. Microwave treatment at 70°C for 4 minutes, and then ultrasonic treatment at 500W for 5 minutes. The extract was centrifuged, and the supernatant was constant volume with 70% ethanol by volume, and the extraction amount of ergothioneine was detected to be the highest when the solid-liquid ratio was 1:50, reaching 2.46 mg / mL.

[0037] The present embodiment has investigated the influence of solid-liquid ratio on the extraction amount of ergothioneine, as figure 1 shown.

Embodiment 3

[0039] The Pleurotus eryngii dry powder obtained in Example 1 is added respectively according to the solid-liquid ratio 1:50 of volume percentage concentration 40%, 55%, 70%, 85%, 100% ethanol, first in microwave power 400W, microwave temperature 70 ℃ Under the condition of microwave treatment for 4 minutes, and then ultrasonic treatment under the condition of ultrasonic power 500W for 5 minutes. The extract is centrifuged, and the supernatant is taken to be 40%, 55%, 70%, 85%, and 100% ethanol to be measured with the volume percentage concentration respectively, and the extraction amount of detection ergothioneine is at 55% ethanol volume percentage concentration. % was the highest, reaching 2.93mg / mL.

[0040] The present embodiment has investigated the impact of ethanol ratio on the extraction amount of ergothioneine, as figure 2 shown.

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Abstract

The invention belongs to the technical field of health food processing, and discloses a method for extracting erythrothioneine from domestic fungi by using ultrasonic-microwave assisted combined vacuum freeze drying. The method comprises the following steps: transversely cutting fresh clean Pleurotus eryngii to form slices, carrying out vacuum freeze drying, crushing the dried slices, and sieving the crushed slices to obtain dry Pleurotus eryngii powder; adding ethanol, and carrying out microwave treatment to obtain a Pleurotus eryngii extract liquid; carrying out ultrasonic treatment on the Pleurotus eryngii extract liquid; and centrifuging the treated liquid, and taking the obtained supernatant to obtain an erythrothioneine extract liquid. Fresh Pleurotus eryngii is dried by adopting a vacuum freeze drying technology, so the Pleurotus eryngii is convenient to preserve, the content and the activity of Pleurotus eryngii basically keep unchanged, full crushing of the Pleurotus eryngii is convenient, and the erythrothioneine extraction yield is increased. The method adopting ultrasonic-microwave assisted extraction has the advantages of simple flow, strong operability, full dissolving of erythrothioneine, short time and high extraction rate. The high-quality erythrothioneine extract liquid can be obtained at a proper extraction temperature; and a nontoxic and harmless extraction agent is adopted, so the extract liquid is safe and environmentally-friendly.

Description

technical field [0001] The invention belongs to the technical field of health food processing, and in particular relates to a method for extracting ergothioneine from edible fungi, in particular to a method for extracting ergothioneine from edible fungi using ultrasonic-microwave assistance combined with vacuum freeze-drying. Background technique [0002] Ergothioneine (ergothioneine, ERT) is a rare compound discovered in ergot in 1909. It exists in many animals and plants, is rich in content, cannot be synthesized by the animal body itself, and can only be ingested from food. It is a rare amino acid. At present, chemically synthesized antioxidants are mostly used at home and abroad, but animal experiments show that they have certain toxicity and carcinogenic effects, while natural antioxidants are safe and non-toxic, and have become a research hotspot. As a natural antioxidant, ergothioneine has many physiological functions such as scavenging free radicals, detoxification,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/84
CPCC07D233/84
Inventor 王杰李亚欢张怡馨秦晓艺
Owner SOUTH CHINA AGRI UNIV
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