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Prawn feed additive containing Rhodobacter Capsulatus and Bacillus subtilis strains, and application thereof

A technology of capsulated rhodobacter and bacillus, applied in bacteria, application, animal feed, etc., can solve problems such as frequent breeding diseases and deterioration of breeding water

Inactive Publication Date: 2016-09-14
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve many problems that the existing high-density aquaculture model easily leads to frequent aquaculture diseases and serious deterioration of aquaculture water, the present invention provides a prawn feed containing capsulated rhodobacter and bacillus strains

Method used

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  • Prawn feed additive containing Rhodobacter Capsulatus and Bacillus subtilis strains, and application thereof
  • Prawn feed additive containing Rhodobacter Capsulatus and Bacillus subtilis strains, and application thereof
  • Prawn feed additive containing Rhodobacter Capsulatus and Bacillus subtilis strains, and application thereof

Examples

Experimental program
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Embodiment 1

[0045] The Litopenaeus vannamei used in this example was taken from Xinyongfeng Prawn Farm in Hangu District, Tianjin;

[0046] The 2216E medium used in this example was prepared with a nine-salt solution;

[0047] The 2216E medium used in this example includes the weight ratio of the following components: peptone 5g / L, yeast extract 1g / L, iron phosphate 0.1g / L, agar 20g / L pH7.6-7.8.

[0048] The indicator bacterium described in this embodiment is Vibrio anguillarum ( Vibrio anguillarum ), purchased from the Institute of Microbiology, Chinese Academy of Sciences, and preserved in the Hydrobiology Laboratory of Tianjin Normal University.

[0049] In this embodiment Rhodobacter capsulatus Screening method for R3 strain:

[0050] Isolation and purification of bacterial strains: Under aseptic conditions, take the whole intestine of prawns in a homogenizer, grind in an ice bath, and use nine salt solution to grind the grinding solution for 10 -1 ~10 -8 Gradient dilution; sel...

Embodiment 2

[0053] This embodiment has the activity of producing digestive enzymes Rhodobacter capsulatus R3 strain and Bacillus The screening method of the B1 bacterial strain, its separation and purification method are the same as in Example 1, the only difference is that the 2216E medium in Example 1 is changed to the Bacillus isolation medium, and will not be repeated here. Wherein the bacillus isolation medium comprises the weight ratio of the following components: peptone 10g / L, yeast extract 5g / L, glucose 3.5g / L, NaCl 5g / L, K 2 HPO 4 0.5g / L, MgSO 4 3g / L, MnSO 4 0.025g / L, agar 15g / L pH7.4~7.6.

[0054] The digestive enzyme activity test was carried out on the purified strain:

[0055] A. Transfer the purified strain to the liquid culture medium at 28°C for constant temperature shaking culture overnight, use sterile tweezers to pick up a piece of sterile filter paper and place it in the culture medium to soak the bacteria solution, and place it in the prepared skim milk med...

Embodiment 3

[0062] The present invention obtained by choosing the above-mentioned embodiment has antibacterial activity and produces digestive enzyme activity Rhodobacter capsulatus R3 strain was identified and analyzed, the specific identification and analysis methods and corresponding identification results are as follows.

[0063] Adopt the method screening of above-mentioned embodiment to have antibacterial activity and produce digestive enzyme activity Rhodobacter capsulatus The R3 strain was inoculated on a 2216E medium plate, cultured upside down at 28°C for 24 hours, and the growth state of the colony was observed. For the strain morphology and physiological and biochemical tests, refer to the Industrial Microbiology Test Technical Manual (Zhuge Jian, Wang Zhengxiang, Industrial Microbial Test Technical Manual [M], China Light Industry Press, 1994) and Bergey's Bacteria Identification Manual (R.E Buchanan, N.E Gibbons, Bergey's Bacteria Identification Manual [M], Science Pre...

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Abstract

The invention discloses a prawn feed additive containing Rhodobacter Capsulatus and Bacillus subtilis strains. The prawn feed additive is composed of a prawn feed, a Rhodobacter Capsulatus strain fermentation liquid, a Bacillus subtilis strain fermentation liquid and sodium alginate, wherein 10g of sodium alginate is added to every 100g of the prawn feed, and culturing is carried out until the OD600 is 0.6, the total volume of the Rhodobacter Capsulatus strain fermentation liquid and the Bacillus subtilis strain fermentation liquid is 20mL and the final concentration is 3-6 * 10<7> / g. A result of feeding with prawn feeds prepared by using Rhodobacter Capsulatus and Bacillus subtilis as feed additives shows that the prawns fed with the feeds containing Rhodobacter Capsulatus and Bacillus subtilis have greatly higher growth speed and obviously high immunity than prawns fed with common feeds; and the feeds prepared by using Rhodobacter Capsulatus and Bacillus subtilis have the advantages of short production period, low production cost, easy control of growth conditions, high antibiotic activity, obviously enhanced immunity, high industrial production potential and good production application prospect.

Description

[0001] The present invention is funded by the 973 project "Immunological approaches and key technical principles for the prevention and treatment of prawn white spot syndrome", project number 2012CB114405, and Tianjin Normal University Biology Outstanding Young Teacher Funding Project, project number ZX110QN008. technical field [0002] The invention belongs to the technical field of bioengineering, and relates to a bacterial strain with antibacterial and digestive enzyme-producing activities, a screening method and application thereof, more specifically a prawn feed containing capsulated rhodobacter and bacillus strains and an application thereof. Background technique [0003] Litopenaeus vannamei ( Litopenaeus vannamei ), also known as Penaeus vannamei ( White Prawn ), Litopenaeus vannamei is delicious in meat, and it is also known as the world's three largest shrimps together with Chinese prawns and Penaeus monodon, and it plays an extremely important role in my country'...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23K20/22C12N1/20C12R1/07C12R1/01
CPCC12N1/205C12R2001/01C12R2001/07
Inventor 左志晗孙金生窦春萌李艳红邵迎春
Owner TIANJIN NORMAL UNIVERSITY
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