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Hydrogen sulfide detection sensor, preparation method thereof, quantitative detection method of hydrogen sulfide, and qualitative detection method of hydrogen sulfide in cells

A detection sensor and qualitative detection technology, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of inapplicable hydrogen sulfide detection and fluorescence imaging, inability to resist photobleaching, and decrease in fluorescence intensity, and achieve excellent environmental protection characteristics and solve The effect of strong toxicity and short preparation time

Active Publication Date: 2016-09-07
ANHUI NORMAL UNIV
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Problems solved by technology

However, so far, most of the fluorescent probes used are organic fluorescent dyes and quantum dots, which are highly toxic and cannot resist photobleaching. Under the irradiation of fluorescent lamps, the fluorescence intensity will be greatly reduced. It is not suitable for intracellular hydrogen sulfide detection and fluorescence imaging, and they need copper to quench the fluorescent probe first, and then add hydrogen sulfide to increase the fluorescence to detect the ternary system of hydrogen sulfide. The process is cumbersome and the error bigger

Method used

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  • Hydrogen sulfide detection sensor, preparation method thereof, quantitative detection method of hydrogen sulfide, and qualitative detection method of hydrogen sulfide in cells
  • Hydrogen sulfide detection sensor, preparation method thereof, quantitative detection method of hydrogen sulfide, and qualitative detection method of hydrogen sulfide in cells
  • Hydrogen sulfide detection sensor, preparation method thereof, quantitative detection method of hydrogen sulfide, and qualitative detection method of hydrogen sulfide in cells

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preparation example Construction

[0032] The invention provides a method for preparing a hydrogen sulfide detection sensor, comprising:

[0033] 1) mixing ethylenediaminetetraacetic acid disodium salt, soluble copper salt and water to form an aqueous solution of a copper complex;

[0034] 2) The aqueous solution of the copper complex is subjected to hydrothermal reaction, filtered, centrifuged, and dialyzed to obtain the liquid in the dialysis bag to obtain an aqueous solution of copper-doped carbon quantum dots;

[0035] 3) An aqueous solution of copper-doped carbon quantum dots was mixed with a buffer solution to prepare a hydrogen sulfide detection sensor.

[0036] In step 1) of the present invention, the consumption of each material can be selected in a wide range, but in order to improve the productive rate of the copper complex that makes, preferably, in step 1), relative to 3.7g of ethylene di Aminetetraacetic acid disodium salt, the amount of soluble copper salt is 1.5-2.5g, and the amount of water is...

Embodiment 1

[0061] 1) At 25°C, mix 3.7224g of ethylenediaminetetraacetic acid disodium salt, 1.7048g of copper chloride and 30ml of water for 8 minutes to form an aqueous solution of copper complex;

[0062] 2) The aqueous solution of the above-mentioned copper complex was subjected to hydrothermal reaction at 200°C for 4 hours, filtered, centrifuged, and dialyzed for 76 hours, and the liquid in the dialysis bag (the molecular weight cut-off of the dialysis bag was 700) was taken to prepare copper-doped carbon An aqueous solution of quantum dots;

[0063] 3) Mix the aqueous solution of the copper-doped carbon quantum dots with the phosphate buffer solution with a pH of 7.4 at a volume ratio of 1:2 to prepare the hydrogen sulfide detection sensor A1.

Embodiment 2

[0065] 1) At 20°C, mix 3.7224g of ethylenediaminetetraacetic acid disodium salt, 1.5g of copper chloride and 20-40ml of water for 5 minutes to form an aqueous solution of copper complex;

[0066] 2) The aqueous solution of the above-mentioned copper complex was subjected to hydrothermal reaction at 180°C for 2 hours, filtered, centrifuged, and dialyzed for 76 hours, and the liquid in the dialysis bag (the molecular weight cut-off of the dialysis bag was 500) was taken to prepare copper-doped carbon An aqueous solution of quantum dots;

[0067] 3) Mix the aqueous solution of the copper-doped carbon quantum dots with the phosphate buffer solution with a pH of 7.2 according to a volume ratio of 1:1 to prepare the hydrogen sulfide detection sensor A2.

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Abstract

The invention discloses a hydrogen sulfide detection sensor, a preparation method thereof, a quantitative detection method of hydrogen sulfide, and a qualitative detection method of hydrogen sulfide in cells. The preparation method comprises the following steps: (1) preparing a water solution of copper complex by mixing disodium EDTA, soluble copper salts, and water; (2) subjecting the water solution of copper complex to hydrothermal reactions, filtration, centrifugation, and dialysis to obtain a water solution of copper doped carbon quantum dots in the dialysis bag; (3) mixing the water solution of copper doped carbon quantum dots with a buffer solution to obtain the hydrogen sulfide detection sensor. The hydrogen sulfide detection sensor can carry out quantitative determination on a hydrogen sulfide solution and qualitative detection on hydrogen sulfide in cells.

Description

technical field [0001] The invention relates to the detection of hydrogen sulfide, in particular to a hydrogen sulfide detection sensor and a preparation method thereof, a quantitative detection method of hydrogen sulfide and a qualitative detection method of intracellular hydrogen sulfide. Background technique [0002] Hydrogen sulfide has recently been found to be a novel signal transmitter in living organisms, which has attracted widespread attention. It has been recognized as the third most important gas signal molecule after two gas signal molecules, nitric oxide and carbon monoxide. Endogenous hydrogen sulfide is mainly synthesized by enzymatic reactions through biological methods, and can participate in various physiological processes such as anti-oxidation, anti-inflammation, and apoptosis. In addition, different concentrations of hydrogen sulfide are also closely related to the exploration of pathological processes. In order to more accurately explore the changes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 高玲玲赵云朱昌青杜金艳蔡方平
Owner ANHUI NORMAL UNIV
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