Composite microbial inoculant for degrading zearalenone and preparation method thereof

A technology of zearalenone and compound bacterial agents, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of nutrient loss, low detoxification rate, time-consuming and labor-intensive problems, and achieve cost-effective Low, will not cause secondary pollution, and improve the effect of degradation rate

Active Publication Date: 2016-09-07
FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

[0003] Zearalenone has an estrogenic effect, can cause acute and chronic poisoning of animals, cause abnormal reproductive function and even death of animals, and can cause huge economic losses to livestock farms
The traditional methods for removing erythralenone mainly include adsorption, extraction, and heat treatment, but they have the disadvantages of time-consuming and laborious, low detoxification rate, and easy loss of nutrients

Method used

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  • Composite microbial inoculant for degrading zearalenone and preparation method thereof
  • Composite microbial inoculant for degrading zearalenone and preparation method thereof

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preparation example Construction

[0020] A preparation method for a composite microbial agent for degrading zearalenone, comprising:

[0021] S1. Culture Paenibacillus, Rhodococcus, Pseudomonas putida, Micrococcus luteus and Acinetobacter baumannii respectively to obtain Paenibacillus bacteria liquid, Rhodococcus bacteria liquid, Pseudomonas putida bacteria liquid, Micrococcus luteus and Acinetobacter baumannii bacteria liquid.

[0022] Among them, Paenibacillus sp. comes from the strain preservation number DSM 11730, Rhodococcus sp. comes from the strain preservation number NCIB 9160, Pseudomonas putida comes from the strain preservation number ATCC 17514, Garcinia cambogia Micrococcus luteus is from strain deposit number NCIB 8166, and Acinetobacter baumannii is from strain deposit number ATCC 15308.

[0023] Specifically, the Paenibacillus and Micrococcus luteus are inserted into the Paenibacillus medium and the Micrococcus luteus medium respectively, and cultured at a temperature of 27-35° C. for 45-50 ho...

Embodiment 1

[0038] In the present embodiment, a preparation method of a composite microbial agent for degrading zearalenone comprises:

[0039] First, insert Paenibacillus from strain preservation number DSM 11730 into a Paenibacillus culture medium consisting of 8 g of peptone, 13 g of beef extract, 14 g of sodium chloride, and 1000 mL of distilled water, at a pH of 7.0 and a temperature of 28°C conditions for 48 hours.

[0040] The rhodococcus from the strain preservation number NCIB 9160 was inserted into the rhodococcus medium consisting of 3 g of glucose, 3 g of yeast powder, 9 g of malt extract, 1.5 g of calcium carbonate and 1000 mL of distilled water, and cultured at a temperature of 28 ° C for 48 Hour.

[0041] Pseudomonas putida from the strain preservation number ATCC 17514 was inserted into a mixture consisting of 18 g of glucose, 2.5 g of DL-2-amino-2-thiazoline-4-carboxylic acid, 4 g of corn steep liquor, 2 g of urea, and 1 g of sodium chloride. , 0.05 g of manganese sulfa...

Embodiment 2

[0054] In the present embodiment, a preparation method of a composite microbial agent for degrading zearalenone comprises:

[0055] First, insert Paenibacillus from the strain preservation number DSM 11730 into a Paenibacillus culture medium consisting of 12g of peptone, 17g of beef extract, 17g of sodium chloride and 1050mL of distilled water, at a pH of 7.2 and a temperature of 35°C conditions for 48 hours.

[0056] The rhodococcus from the strain preservation number NCIB 9160 was inserted into the rhodococcus medium consisting of 5 g of glucose, 5 g of yeast powder, 11 g of malt extract, 2.5 g of calcium carbonate and 1050 mL of distilled water, and cultured at a temperature of 30 ° C for 50 Hour.

[0057] Pseudomonas putida from the strain preservation number ATCC 17514 was inserted into a mixture consisting of 22 g of glucose, 3.5 g of DL-2-amino-2-thiazoline-4-carboxylic acid, 6 g of corn steep liquor, 4 g of urea, and 2 g of sodium chloride. , 0.15 g of manganese sulf...

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Abstract

The invention discloses a preparation method of a composite microbial inoculant for sludge treatment. The method comprises the following steps: respectively culturing Paenibacillus, Rhodococcus, Pseudomonas putida, Micrococcus luteus and Acinetobacter baumannii to obtain a Paenibacillus solution, a Rhodococcus solution, a Pseudomonas putida solution, a Micrococcus luteus solution and an Acinetobacter baumannii solution; mixing the Paenibacillus solution, Rhodococcus solution, Pseudomonas putida solution, Micrococcus luteus solution and Acinetobacter baumannii solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading zearalenone. The composite microbial inoculant can efficiently degrade zearalenone, and has the advantages of low cost and no secondary pollution.

Description

technical field [0001] The invention relates to the technical field of zearalenone degradation, in particular to a composite microbial agent for degrading zearalenone and a preparation method thereof. Background technique [0002] Zearalenone (Zearalenone), also known as F-2 toxin, was first isolated from corn with head blight. The toxin-producing bacteria of zearalenone are mainly strains of the genus Fusarium, such as F. graminearum and F. tricinctum. Zearalenone mainly contaminates grains such as corn, wheat, rice, barley, millet and oats. Among them, the positive detection rate of corn is 45%, and the highest toxic content can reach 2909 mg / kg; the detection rate of wheat is 20%, and the toxic content is 0.364-11.05 mg / kg. The heat resistance of zearalenone is strong, and it can be completely destroyed after being treated at 110°C for 1 hour. [0003] Zearalenone has the effect of estrogen, which can cause acute and chronic poisoning of animals, cause abnormal reprodu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/40C12R1/265C12R1/01
CPCC12N1/20
Inventor 郭鹏王轶程薇
Owner FARM PROD PROCESSING & NUCLEAR AGRI TECH INST HUBEI ACAD OF AGRI SCI
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