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Pseudomonas guariconensis strain ST4 and application thereof in prevention and treatment of sugarcane smut

A technology for Pseudomonas and smut, applied in the application, bacteria, fungicides and other directions, can solve the problems of few chemical control research reports, long time consumption, high input cost, and achieves the prevention and control of sugarcane whip smut and environmental protection. friendly effect

Active Publication Date: 2016-09-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main control measures for sugarcane whip smut are: 1. Cultivate and popularize resistant varieties, which can better prevent and control the occurrence of smut. However, the cultivation of new varieties often requires a large amount of manpower and material resources, which consumes Long time, but the effect is not optimistic
Moreover, the physiological race of smut changes rapidly, and it is easy to turn resistant varieties into susceptible varieties.
2. Integrated agricultural control, integrated agricultural control is the basic method to prevent all crop diseases and insect pests, but the input cost is high
3. Chemical control. Chemical control is currently the mainstream method of pest control and the most effective method. However, due to the unique properties of sugarcane, there are very few research reports on chemical control. Effective Chemicals for Sugarcane Whip Smut
Four, biological control, biological control is the use of biological methods to achieve the purpose of preventing and controlling plant diseases and insect pests. In recent years, there have been some reports on the biological control of sugarcane whip smut, but mainly lie in the screening that can inhibit the germination of smut teliospores. Inhibition of bacterial growth and other stages, no application case of biological control of sugarcane whip smut has been found

Method used

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  • Pseudomonas guariconensis strain ST4 and application thereof in prevention and treatment of sugarcane smut
  • Pseudomonas guariconensis strain ST4 and application thereof in prevention and treatment of sugarcane smut
  • Pseudomonas guariconensis strain ST4 and application thereof in prevention and treatment of sugarcane smut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Isolation and screening of embodiment 1 Pseudomonas ST4

[0030] Soil sample collection: Separated soil samples were collected around the vegetable rhizosphere in the suburbs of Shantou City, Guangdong Province on May 2, 2013. The surface soil was scraped off, and 5-10g of vegetable rhizosphere soil samples at a depth of about 10cm were collected, stored in bags and brought back to Guangzhou. Perform strain isolation.

[0031] Isolation of bacterial strains: Isolation was carried out by dilution plate coating method, weighing 1g of isolated soil sample, adding 9mL of sterile water, shaking overnight at 28°C. Take 1mL of culture solution and dilute it 10-fold until 10 -8 , draw 100μL10 respectively -4 , 10 -6 , 10 -8 Spread the diluted solution on the prepared LB plate and culture it at 28°C until the colony grows, then pick a single colony and culture it in a 1.5mL centrifuge tube for use.

[0032]Strain screening: use the plate confrontation method to screen out t...

Embodiment 2

[0033] The biological characteristic analysis of embodiment 2 biocontrol bacteria ST4

[0034] In order to better study the biocontrol potential of the strain ST4 obtained in Example 1, we conducted in-depth research on some biological characteristics of the strain. The specific operation is as follows:

[0035] Use an inoculation loop to pick a single ST4 colony to streak and inoculate in nutrient medium and PDA medium respectively, culture at 28°C for 24 hours, and observe the growth of the colony. The bacterial colony on the nutrient medium is beige, round, slightly concave in the middle, smooth and translucent, with neat edges, diffuse turbidity in the liquid medium, and a large amount of yellow-green pigment on the PDA plate. By scanning electron microscopy, it was found that figure 2 As shown in B, the bacterium has two polar flagella, which shows that the bacterium can move better in the natural environment and better adapt to the needs of different environments, whi...

Embodiment 3

[0038] Identification and evolution analysis of embodiment 3 biocontrol bacteria ST4

[0039] The experiment carried out morphological observation, physiological and biochemical analysis, electron microscope analysis, Biolog system analysis and main gene evolution analysis on the strain ST4 obtained in Example 1. The morphological observation results show that the bacteria are Gram-negative, the cells are rod-shaped, the size is 1.9-3.1×0.8-0.9μm, with 2 polar flagella, and the colonies on the nutrient medium are beige, round, slightly Concave, smooth and translucent surface, neat edges, diffuse turbidity in liquid medium, yellow-green pigment produced on PDA plate. Physiological and biochemical analysis The bacteria are aerobic, motile, non-fermenting bacteria, positive for hydrogen peroxide and oxidase, produce diffusible fluorescent pigments, cannot reduce nitrate, do not use glycerin, can be used at 44°C high temperature, 5% grown under NaCl conditions. The identificatio...

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Abstract

The invention belongs to the technical field of biological prevention and control, and particularly discloses a pseudomonas guariconensis strain ST4 and an application thereof in the prevention and the treatment of sugarcane smut. The strain is preserved in CCTCC (China Center for Type Culture Collection) on September 14, 2015, and the strain preservation number is CCTCC NO. M 2015526. The invention discovers that the strain ST4 has a good inhibition or delay effect on the sexual matching of the sugarcane smut; the formation of dicaryon mycelia is blocked, so that the sporisorium scitaminea cannot normally infect the sugarcane; a novel development resource is provided for replacement of chemical synthetic bactericides by microbes; and the strain ST4 can be used as biopesticide to be developed and utilized.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a Pseudomonas strain ST4 and its application in preventing and controlling sugarcane whip smut. Background technique [0002] Sugarcane is an important sugar crop in the world and also the main sugar crop in my country. It is mainly distributed in Guangdong, Guangxi, Yunnan, Fujian, Hainan and Taiwan. In addition, sugarcane is also a potential renewable energy crop. China is one of the countries where sugarcane is relatively old. As early as the 4th century BC, it was distributed from the Qinling Mountains to Hainan Island in my country. It is an important source of ensuring the safety of my country's sugar industry. However, the occurrence and development of sugarcane whip smut is gradually threatening our country and even the sugarcane planting areas of the world, seriously restricting the development of the sugarcane sugar industry and seriously affecting the safety...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 张炼辉刘诗胤周佳暖陈少华贺飞
Owner SOUTH CHINA AGRI UNIV
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