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Preparation method of bacterial cellulose/functional nanometer particle composite film

A bacterial cellulose and functional nanotechnology, applied in the field of composite materials, can solve the problems of destroying the natural three-dimensional network structure of bacterial cellulose, the inability to directly compound nanoparticles, and the inability to exert excellent properties, etc., to achieve the effect of expanding applications

Inactive Publication Date: 2016-08-31
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above three methods have serious flaws
The slurry composite method destroys the natural three-dimensional network structure of bacterial cellulose and cannot exert its excellent properties
The in situ composite of bulk fibers is limited to the synthesis of small molecules and cannot be directly composited with existing functional nanoparticles
In-situ compounding in the fermentation process is currently the most ideal compounding method, and then only microbial pellets can be obtained, which cannot be used in devices. So far, there is no literature report that bulk membrane materials can be obtained through in-situ fermentation compounding

Method used

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  • Preparation method of bacterial cellulose/functional nanometer particle composite film
  • Preparation method of bacterial cellulose/functional nanometer particle composite film
  • Preparation method of bacterial cellulose/functional nanometer particle composite film

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[0026] The embodiment of the present invention discloses a preparation method of bacterial cellulose / functional nanoparticle composite film, comprising the following steps:

[0027] A), inoculating the bacterial species onto a solid substrate, and forming a cellulose film on the surface of the solid substrate after fermentation;

[0028] B), adding a nutrient solution and a source of functional nanoparticles to the surface of the cellulose membrane, and fermenting;

[0029] C), repeat step B), and obtain bacterial cellulose / functional nanoparticle composite film after culturing.

[0030] In the invention, bacteria are inoculated onto a solid substrate, and nutrient solution and functional nanoparticle sources are added repeatedly during the growth of bacteria, and continuous fermentation is performed to obtain a bacterial cellulose / functional nanoparticle composite membrane.

[0031] In the process of preparing the bacterial cellulose / functional nanoparticle composite membran...

Embodiment 1

[0045] Prepare 500mL aqueous solution containing 10wt% glucose, 1wt% yeast powder, 2wt% calcium carbonate, and 1.5wt% agar, stir to dissolve and boil, divide into five 250mL beakers, seal the beakers with tissue culture film, and autoclave Sterilize the pot, cool and stand to obtain a plane; prepare an aqueous solution containing 5wt% glucose and 1wt% yeast powder; use sterile water to prepare a 1mg / mL graphene oxide solution;

[0046] Inoculate the strain of Gluconacetobacter xylinum on the above-mentioned flat medium, put it into an incubator, and cultivate it at 28°C for 36h;

[0047] Transfer the beaker from a constant temperature incubator to a sterile table, add 0.5mL of nutrient solution with a pipette gun, shake gently to spread evenly, and add 0.4mL of the above graphene oxide aqueous solution with a pipette gun, shake gently to spread evenly, and transfer to a constant temperature Incubator; repeat the above operation once every 8 hours, and cultivate for 10 days;

...

Embodiment 2

[0053] Prepare 500mL aqueous solution containing 10wt% glucose, 1wt% yeast powder, 2wt% calcium carbonate, and 1.5wt% agar, stir to dissolve and boil, divide into five 250mL beakers, seal the beakers with tissue culture film, and autoclave Sterilize, cool and stand to obtain a plane; prepare an aqueous solution containing 5wt% glucose and 1wt% yeast powder; prepare a 2mg / mL carbon nanotube solution and sterilize it with an autoclave;

[0054] Inoculate the Gluconacetobacter xylinum species on the above-mentioned plane medium, put it into an incubator, and cultivate it at 28°C for 36h;

[0055] Transfer the beaker from the constant temperature incubator to the sterile table, add 0.5mL of nutrient solution with a pipette gun, shake gently to spread evenly, and add 0.4mL of the above carbon nanotube aqueous solution with a pipette gun, shake gently to spread evenly, and transfer to constant temperature Incubator; repeat the above operation once every 8 hours, and cultivate for 10...

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Abstract

The invention provides a preparation method of a bacterial cellulose / functional nanometer particle composite film. The preparation method comprises inoculating a solid base with a strain, forming a cellulose membrane on the surface of the solid base after fermentation, adding a nutrient solution and a functional nano-particle source to the surface of the cellulose membrane, carrying out fermentation, repeating the above processes and carrying out culture for several days to obtain the bacterial cellulose / functional nanometer particle composite film with a certain thickness. The preparation method realizes compounding of bacterial cellulose and functional nanometer particles and prepares a large-block composite functional membrane under this premise of no damage to a bacterial cellulose three-dimensional network and a macrostructure. The preparation method has the advantages of simple processes, low cost, environmental friendliness and large scale production, effectively broadens a bacterial cellulose application area and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of composite materials, in particular to a method for preparing a bacterial cellulose / functional nanoparticle composite film. Background technique [0002] Bacterial cellulose is a general term for cellulose synthesized by certain microorganisms in the genus Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. As a new type of biological material, bacterial cellulose has many superior properties, such as: good microscopic three-dimensional network structure, good crystallinity, high chemical purity, high hydrophilicity, high biocompatibility and good mechanical properties, etc. , so it has broad application prospects in many fields. [0003] The multifunctionalization of bacterial cellulose can be realized by combining bacterial cellulose with functional nanoparticles, and advanced multifunctional composite materials can be obtained. At present, the methods for preparing bacterial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N11/00
CPCC12P19/04C12N11/00
Inventor 俞书宏管庆方梁海伟
Owner UNIV OF SCI & TECH OF CHINA
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