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Building method of soybean variety SSR (simple sequence repeat) fingerprint identification card

A fingerprint and construction method technology, which is applied in the field of construction of SSR fingerprint identification cards of soybean varieties, can solve problems such as unfavorable comparison identification and popularization and application, errors in allelic variation recording methods, etc., and achieve easy archival, recording and analysis. Compare, resolve property rights disputes, easy to operate

Inactive Publication Date: 2016-08-24
JILIN ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for constructing a soybean variety SSR fingerprint ID card, which can not only effectively solve the problems in the traditional method of allelic variation recording, which are prone to errors, and the large number of marker combinations is not conducive to subsequent comparison, identification and promotion. It can provide scientific, accurate and effective technical support for the rapid identification and accurate evaluation of new soybean varieties.

Method used

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  • Building method of soybean variety SSR (simple sequence repeat) fingerprint identification card
  • Building method of soybean variety SSR (simple sequence repeat) fingerprint identification card
  • Building method of soybean variety SSR (simple sequence repeat) fingerprint identification card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] Example: Construction of SSR Fingerprint ID Card of Newly Breeding Soybean Varieties in Jilin Province

[0018] (1) Extraction of genomic DNA of soybean varieties

[0019] Genomic DNA was extracted from 0.1 g of young soybean leaves by using the improved CTAB method from 98 soybean varieties approved in Jilin Province from 2005 to 2012, diluted to 10-15 ng / μl, and stored in a -20°C refrigerator for later use .

[0020] (2) PCR amplification and polyacrylamide gel electrophoresis detection

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Abstract

The invention relates to a building method of a soybean variety SSR (simple sequence repeat) fingerprint identification card. The building method is characterized by comprising steps as follows: firstly, SSR markers are screened preliminarily, core markers required by building of the soybean variety SSR fingerprint identification card are determined with a diversity index as selection basis, overall fingerprint banding patterns of the core SSR markers in the soybean variety are recorded, the banding patterns are subjected to digital coding, finally, the banding patterns of the core SSR markers are connected in series according to a fixed sequence, and SSR fingerprint identification card codes of the soybean variety are obtained. According to the building method of the soybean variety SSR fingerprint identification card, the technical problems that an allelic variation recording mode is prone to error generation, a larger number of SSR marker combinations are adverse to follow-up comparison identification, popularization and application and the like in a traditional method are effectively solved, and the building method has the advantages that varieties are simple and clear to distinguish, data are easy to archive, record, analyze and compare and the like, and can provide scientific and effective reference base for fast and accurate identification of a new soybean variety, evaluation of genetic resources and solving of variety property right disputes.

Description

technical field [0001] The invention relates to a method for constructing a soybean variety SSR fingerprint ID card, and belongs to the technical field of crop variety molecular identification. Background technique [0002] As an important step in seed production, variety identification is an important means to protect new varieties, prevent counterfeit and inferior varieties from entering the market, and safeguard the interests of farmers. In recent years, with the repeated use of excellent parents and the lack of new germplasm, some soybean varieties have similar blood relationship and similar phenotypic characteristics. Although it is simple and economical, it takes a long period of time, and the phenotypic characteristics are easily affected by the environment, and identification errors occur from time to time, which cannot meet the needs of today's variety identification (Chinese Journal of Oil Crops, 2013 / 35(3 ) / / 231~239). With the development of molecular biology te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113C12Q2565/125
Inventor 陈亮王曙明范旭红孟凡凡郑宇宏孙星邈张云峰王明亮
Owner JILIN ACAD OF AGRI SCI
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