Medical antimicrobial gauze
A medical and gauze technology, applied in the fields of medical science, absorbent pads, bandages, etc., can solve the problem of broad-spectrum sterilization that needs to be improved, and achieve the effect of promoting wound healing, mild and non-irritating medicine, and high patient acceptance.
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Embodiment 1
[0011] Example 1 Obtained by screening of antibacterial polypeptides
[0012] The ginseng genome sequence obtained through pre-screening, looking for small open reading frames with potential coding functions, and translating these small open reading frames into corresponding amino acid sequences, using three-dimensional structure analysis software to screen antimicrobial peptides from them, and screening antimicrobial peptides based on predictions Peptide sequence, using solid-phase synthesis method to synthesize part of the peptides, and amidate the C-terminus, N-terminus does not contain methionine, prepare antibacterial peptides, carry out corresponding functional verification, screened 22 antibacterial peptides, named WSJ- 1~22. Their sequences are shown in SEQ ID NO: 1-22 respectively.
Embodiment 2
[0013] Example 2 Verification of the effect of antibacterial peptides
[0014] The WSJ-1 polypeptide was dissolved in distilled water to a concentration of 2mg / ml, and the antibacterial activity was detected by the agarose diffusion method. The strains used in the agarose diffusion method are Chlamydia trachomatis, Staphylococcus aureus, Neisseria gonorrhoeae, Leucorrhea, and Candida albicans. After the resuscitation of the strains, they were inoculated into the corresponding medium and cultivated overnight, and then 50 microliters of the bacterial solution were applied to the solid medium. After standing the bacteria-carrying liquid to absorb, add 10 microliters of peptide solution with a concentration of 2mg / ml. After culturing at 37°C for 16 hours, observe the presence and size of the inhibition zone. At the same time, observe whether there is any change in the inhibition zone within a week, and judge the length of inhibition time. The results are shown in Table 1.
[0015] ...
Embodiment 3
[0020] Example 3 Preparation of antibacterial gauze
[0021] Dip the soluble gauze grey cloth into an aqueous solution of polyethyleneimine. The concentration of polyethyleneimine in the aqueous solution is 10mg / ml, containing 0.14mol / L NaCl and soaked in reciprocating oscillation for 40 minutes. The polymer bottom layer will be formed on the inner and outer surfaces of the soluble gauze grey cloth. ; Then placed in 100mL methanol solution of 4,4'-diphenyldithiol with a concentration of 0.01% by mass for 5 hours, the soluble gauze fabric after the reaction was fully immersed in methanol and dried in vacuum, and SeqIDNO:1 The antibacterial peptides are respectively reacted under the action of horseradish peroxidase and hydrogen peroxide to obtain a soluble gauze cloth with antibacterial peptides on the surface.
[0022] The antibacterial peptide of any one of SeqIDNO: 2-22 is prepared according to the gauze preparation method of sequence 1 to prepare the corresponding gauze coated w...
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