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Use of blood metabolism marker and depression detecting kit

A technology for detection kits and markers, applied in the medical field, can solve the problems of difficult diagnosis and long diagnosis cycle, and achieve the effect of simplifying the diagnosis process, high accuracy, and huge market development prospects

Active Publication Date: 2016-07-13
TIANJIN SUNNYPEAK BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has a long diagnostic cycle and is difficult to diagnose, and it may even take 2 years to diagnose

Method used

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  • Use of blood metabolism marker and depression detecting kit
  • Use of blood metabolism marker and depression detecting kit
  • Use of blood metabolism marker and depression detecting kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Screening of blood metabolic markers for diagnosis of depression

[0037] 1. Extraction of metabolome from blood

[0038]With the consent of the blood donors, blood was collected from normal people and patients with clinically confirmed depression by the dried blood spot method, with at least 10 cases in each group. Take dried blood spots, use a paper puncher, take blood samples, add 90 μl metabolite extract (methanol-acetonitrile-water, 40:40:20) and 10 μl stable isotope internal standard, vortex mix. Let stand on ice for 10 minutes. The mixture was centrifuged at low temperature and high speed for 15 minutes (16,000 g, 4° C.). After centrifugation, 50 μl of supernatant was taken for metabolome analysis in blood.

[0039] 2. Targeted metabolomics analysis based on LC-MS / MS

[0040] Inject 10 μl of metabolic extract into LunaNH via an autosampler 2 In a chromatographic column (250mm×2mm, 5μm, Phenomenex, CA), the metabolites in the blood samples were sepa...

Embodiment 2

[0047] A depression detection kit, which contains: standard pure liquid of metabolic markers (10mg / ml, 1 milliliter); 13 C stable isotope internal standard solution (1mg / ml, 100 microliters); metabolite extraction solvent (10ml); analytical chromatographic column (1 piece) and quality control standard serum (100 microliters).

[0048] The preparation method of the kit is as follows:

[0049] Standard pure product solution of metabolic markers: take metabolite standard product powder, accurately weigh 10 mg, dissolve in 1 ml of methanol, and prepare 10 mg / ml pure metabolic marker solution.

[0050] 13C stable isotope internal standard solution: take 13C stable isotope extraction powder, accurately weigh 1 mg, dissolve in 1 ml of methanol, and prepare 1 mg / ml 13C stable isotope internal standard solution.

[0051] Metabolite extraction solvent: Accurately measure 5ml of chromatographically pure methanol, add 5ml of chromatographically pure water, and mix well to obtain a metabo...

Embodiment 3

[0055] Clinical example of depression detection kit diagnosis of depression

[0056] The blood of 100 outpatients was randomly collected from a certain outpatient clinic, collected on dried blood spots, and diagnosed with depression using the depression detection kit described in Example 2.

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Abstract

The invention provides a use of a blood metabolism marker and a depression detecting kit. The blood metabolism marker can be used for preparing a reagent or an apparatus for diagnosing depression, for example a kit which comprises standard pure liquor with concentration being 10mg / ml of the metabolism marker, 13C stable isotope internal standard liquor, methanol-aqueous solution with a volume ratio being 1 to 1, one analytical chromatographic column and quality control standard serum; the kit can be used for accurately measuring content of 14 screened metabolism markers in blood, and is utilized for early diagnosing the depression with specificity being 86.8%, sensitivity being 96.8% and accuracy rate being 93.0%, so that the diagnosis of the depression does not depend on a questionnaire completely for subjective judgment, and therefore, the diagnosis accuracy is greatly improved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the use of blood metabolism markers and a depression detection kit. Background technique [0002] Depression, also known as depressive disorder, is a mental disease characterized by depressed mood, accompanied by listlessness, loss of appetite and sleep disturbance, which seriously endanger human health. Depression has the characteristics of long onset time, difficulty in curing, high disability rate and suicide rate, which has brought heavy burdens to individuals, families and society. At present, there are at least 320 million patients with various types of depression in the world, which is the fourth largest disease in the world. [0003] Depression is a complex mental illness, and the specific mechanism of its pathogenesis is not yet fully understood. The onset of depression is closely related to the patient's diet, sleep and living and working environment. Therefore, tra...

Claims

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Application Information

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IPC IPC(8): G01N33/96
CPCG01N33/96G01N2800/304
Inventor 王旭李克峰
Owner TIANJIN SUNNYPEAK BIOTECH CO LTD
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