Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for simultaneous detection of ascorbic acid, dopamine, uric acid, tryptophan and nitrite

A technology of ascorbic acid and nitrite, applied in the direction of material electrochemical variables, etc., can solve the problems of cumbersome sample processing process, and achieve the effect of miniaturization, good stability and low detection limit

Active Publication Date: 2019-03-01
TIANJIN UNIVERSITY OF TECHNOLOGY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In clinical applications, either different detection methods are used for different substances, or the sample processing process is cumbersome, or biological substances that are easily inactivated such as enzymes are used for labeling, which has a negative impact on the detection speed and effect.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for simultaneous detection of ascorbic acid, dopamine, uric acid, tryptophan and nitrite
  • A method for simultaneous detection of ascorbic acid, dopamine, uric acid, tryptophan and nitrite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of working electrode

[0029] ① Configure the concentration of 0.6mol / L Ni(NO 3 ) 2 Ethanol solution, and drop-coated on the surface of tantalum wire with a diameter of 0.6mm, dried and placed on the sample stage of the DC arc plasma spray chemical vapor deposition system, adjust the height of the sample stage so that it is about 1.5cm away from the plasma injection port .

[0030] ②Close the reaction chamber and evacuate the system. When the vacuum degree reaches below 1Pa, the flow rate of 2L / min of hydrogen, 1.5L / min of argon and 200mL / min of methane is introduced, and the reaction pressure is 100Pa~4000Pa. The arc voltage is 50V-70V, the arc current is 80A-130A, the substrate temperature is 500°C-1200°C, and the growth time is 0.5min-30min. After the growth time is 0.5min-30min, the graphene / tantalum wire sample is taken out as the working electrode. In the present invention, when the chamber pressure and pump pressure reach 3000Pa and 13000Pa, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for simultaneously detecting ascorbic acid, dopamine, uric acid, tryptophan and nitrite.The method is implemented by a three-electrode system sensor with a working electrode, a reference electrode and a counter electrode.The working electrode is made of graphene / tantalum, graphene / boron-doped diamond films and graphene / titanium; the reference electrode can be a saturated calomel electrode or an Ag / AgCl electrode or a mercury / mercurous sulfate electrode; the counter electrode is a platinum sheet electrode.The method includes acquiring working curves by the aid of mixed solution of five to-be-detected materials with different concentrations and differential pulse voltammetry; diluting human serum; adjusting the concentrations until the concentrations are within the ranges of the working curves; comparing peak currents obtained by the aid of the differential pulse voltammetry to the working curves to obtain the concentrations of the five to-be-detected materials in the serum.The method has the advantages that characteristics of the concentrations of the five to-be-detected materials can be quickly simultaneously detected, and the method is high in sensitivity and low in detection limit.

Description

technical field [0001] The invention relates to an electrochemical method for simultaneously detecting five substances such as ascorbic acid, dopamine, uric acid, tryptophan and nitrite. Background technique [0002] Five substances, ascorbic acid, dopamine, uric acid, tryptophan and nitrite, play an important role in the metabolism of the human body. Through the detection of these substances, it is possible to judge scurvy, schizophrenia, Alzheimer's disease, diabetes, The content of the above substances contained in the body fluids of patients with liver function decline, food poisoning, etc., and play a preventive role. [0003] At present, redox titration, spectrophotometry, uricase-peroxidase coupling method, fluorescence spectrophotometry, colorimetry, etc. are commonly used in clinical medicine. In clinical applications, either different detection methods are used for different substances, or the sample processing process is cumbersome, or biological substances that ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/48
CPCG01N27/26G01N27/30G01N27/48
Inventor 李明吉高德兰李红姬李翠平曲长庆杨保和
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com