An electrochemical detection method for novel coronavirus 2019-ncov

A technology for 2019-ncov and coronavirus, applied in biochemical equipment and methods, electrochemical variables of materials, measurement/inspection of microorganisms, etc., can solve the problems of low detection sensitivity, time-consuming, high maintenance costs, etc.

Active Publication Date: 2020-10-27
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the routine detection method of 2019-nCoV is basically the fluorescent PCR method, but this method has problems such as expensive equipment, time-consuming, high maintenance cost, and low detection sensitivity.
Electrochemical methods have the advantages of high efficiency, rapidity, sensitivity, and cheapness. However, no research on electrochemical detection of 2019-nCoV has been reported so far.

Method used

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  • An electrochemical detection method for novel coronavirus 2019-ncov
  • An electrochemical detection method for novel coronavirus 2019-ncov

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Experimental program
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Effect test

Embodiment Construction

[0058] A kind of electrochemical detection method of novel coronavirus 2019-nCoV, its steps are as follows:

[0059] (1) Ferric oxide (Fe 3 o 4 ) Preparation of nanospheres

[0060] Add ferric chloride hydrate to ethylene glycol to form a clear solution, add sodium acetate and polyethylene glycol, stir for 50 minutes, put it into a hydrothermal reaction kettle for heating, and cool to room temperature to obtain a black precipitate. The precipitate was washed with absolute ethanol and dried to obtain Fe 3 o 4 nanospheres;

[0061] Wherein the mass volume concentration of ferric trichloride in ethylene glycol is 0.3%, hydrothermal reaction is to react at 180 DEG C for 8 hours, and drying is to process at 70 DEG C for 6 hours. The mass ratio is 4:1, and the mass ratio of polyethylene glycol and ferric chloride is 1:1;

[0062] (2) Gold nanoparticles supported Fe 3 o 4 Nanocomposites (Au@Fe 3 o 4 ) preparation

[0063] Step (1) Fe 3 o 4 Nano-microspheres are dispersed...

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Abstract

The present invention relates to an electrochemical detection method for novel coronavirus 2019-nCoV. The detection method includes the design of three probes targeting two structural domains in the RNA sequence of novel coronavirus 2019-nCoV, the preparation of three substances and Construction of electrochemical sensors. The electrochemical detection of the novel coronavirus 2019-nCoV of the present invention has the significant advantages of simple operation, fast / accurate detection, and low cost.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, in particular to the technical field of electrochemical detection for novel coronavirus (2019-nCoV). Background technique [0002] Novel coronavirus (2019-nCoV) is a new type of coronavirus that has not been found in humans before. It can be transmitted through droplets, aerosols, and contact. Highly contagious and harmful. At present, the conventional detection method of 2019-nCoV is basically the fluorescent PCR method, but this method has problems such as expensive equipment, time-consuming, high maintenance cost, and low detection sensitivity. Electrochemical methods have the advantages of high efficiency, rapidity, sensitivity, and low cost. However, no research on electrochemical detection of 2019-nCoV has been reported so far. Contents of the invention [0003] The purpose of the present invention is to provide a method for detecting 2019-nCoV that is convenient, highly sensitive...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6825
CPCG01N27/3277G01N27/3278G01N27/48Y02A50/30
Inventor 李灿鹏赵卉张亚平刘凤谢伟
Owner YUNNAN UNIV
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