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Genetic recombined phycocyanobilin serving as optical sensitization material and application thereof

A sensitizing material, phycocyanobilin technology, applied in the fields of biotechnology and energy, can solve problems such as poor stability, limited application of photosensitive materials, protein variability, etc., and achieve the effects of low cost, high quantum efficiency, and high photovoltaic characteristics

Inactive Publication Date: 2016-07-06
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both phycobiliproteins and phycobilisomes are pigment-protein complexes, which require correct orientation and assembly on the electrode surface to form a good photoelectric conversion efficiency. In addition, due to the shortcomings of proteins such as easy denaturation and poor stability on the electrode surface, serious Limit its application as a photosensitive material

Method used

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  • Genetic recombined phycocyanobilin serving as optical sensitization material and application thereof
  • Genetic recombined phycocyanobilin serving as optical sensitization material and application thereof
  • Genetic recombined phycocyanobilin serving as optical sensitization material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Obtaining of sensitizing material phycocyanin:

[0038] The pET28a-hox1-pcyA strain originally preserved in the laboratory (this strain can use the metabolite heme in Escherichia coli to be converted into phycocyanin by the enzymes Hox1 and PcyA), according to the inoculation volume of 20 μL, inoculate the strain in 5ml In liquid sterile LB medium, culture at 37°C, 170rpm for 12-16h. Then, according to the volume ratio of 1% inoculum, the strain was inoculated in 1L of TB medium, and cultivated at 37°C for OD 600 to 0.8-1.0, then add appropriate amount of IPTG, induce at a low temperature of 18°C, and collect the bacteria after 20 hours.

[0039] Separation and purification of phycocyanin: Take the Escherichia coli obtained in the previous step out of the -80°C refrigerator, add 50-100mL of methanol according to the sample size, vortex and mix well, and then transfer it to a three-necked flask. Wrap it in a water bath at 50°C for 1-2h. After the reaction is completed...

Embodiment 2

[0044] Construction of the photoanode: The photoanode is TiO 2 Electrode, customized TiO by Dalian Qiseguang Technology 2 Semiconductor film thickness of 1:1 "20nmTiO 2 Single layer film (2μm)+200nmTiO 2 Single-layer scattering layer (2μm)". The above photoanode is made by screen printing method and exists on FTO (fluorine-doped tin oxide) glass substrate. The size of the monolithic electrode glass is 12×18mm and the thickness is 2.2mm. TiO 2 Semiconductor film size 6×6mm (effective area 0.36cm 2 ), is a nanoporous structure with a pore size of 1-1000nm.

[0045] Sensitization of the photoanode: the anode electrode was heated at 350° C. for 30 min in a muffle furnace (Yamato Corporation, Japan, Model Since 1889) and cooled naturally and rapidly to 50° C. at room temperature. Immediately soak the titanium dioxide anode electrode in the PCB-ether sensitization solution, absorb it in the dark at room temperature for 24 hours, rinse both sides with absolute ethanol to remove t...

Embodiment 3

[0054] 1) The preparation steps of phycocyanobilin are as described in Example 1.

[0055]2) A gold foil with a nanoporous structure is used as a photoanode, and platinum is used as a counter electrode. After ultrasonic vibration treatment with ethanol and chloroform for 20 minutes, UV ozone disinfection for 10 minutes. A gold foil electrode was soaked in a solution of phycocyanobilin in ether for 24 hours at room temperature in the dark. After adsorption, the sensitized photoanode was rinsed with deionized water to remove weak adsorption, and then dried under nitrogen.

[0056] 3) The assembly of the phycocyanobilin bio-solar cell or light-harvesting device is the same as that described in Example 2.

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Abstract

An optical sensitization material disclosed by the invention is phycocyanobilin prepared by means of genetic engineering. In the sensitization material, a semiconductor electrode is processed by a mode of sensitization of a TiO2 particle electrode, vacuum pumping and drying are carried out in a room temperature, the absorption of the electrode to the sensitization material is completed through electrostatic force absorption and hydrogen bonding interaction, the sensitization material is assembled to a dye-sensitized solar cell, the semiconductor electrode is sensitized so that the conversion of solar energy to electric energy can be directly completed under a light condition, and the function of the phycocyanobilin serving as a novel optical sensitization material is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology and energy, and specifically relates to the application of a gene recombinant phycocyanin as an optical sensitization material. Background technique [0002] Coal, oil, natural gas and other fossil fuels have been widely exploited one after another and widely used in people's production and life. With the increase of people's demand for energy, these non-renewable energy sources that human beings rely on for survival have brought about increasingly severe energy crises and more and more serious environmental pollution. On the one hand, these energy sources are non-renewable. On the other hand, the large amount of carbon dioxide gas produced by the combustion of these fossil fuels will cause the greenhouse effect and accelerate the speed of global warming. In addition, severe environmental pollution and ecological damage caused by mining also threaten the survival of human beings and other living thi...

Claims

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Application Information

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IPC IPC(8): H01G9/20H01G9/042C12P17/16C12R1/19
CPCH01G9/2059C12P17/165Y02E10/542Y02P20/59Y02P20/133
Inventor 葛保胜吝晓君王祥法李焰于道永黄方
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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