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Fusarium oxysporum and application thereof to phytoremediation of heavy metal contaminated soil

A technology of Fusarium oxysporum and Fusarium genus, applied in the field of soil remediation, can solve the problem of not much work, and achieve the effect of both remediation, strong resistance to heavy metals, safe remediation and environmental protection

Active Publication Date: 2016-07-06
湖南省环境保护科学研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is not much work done in this field on the synergistic remediation of microorganisms and plants in the field of heavy metal pollution remediation.

Method used

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  • Fusarium oxysporum and application thereof to phytoremediation of heavy metal contaminated soil
  • Fusarium oxysporum and application thereof to phytoremediation of heavy metal contaminated soil
  • Fusarium oxysporum and application thereof to phytoremediation of heavy metal contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Configure PDA liquid medium containing cadmium, lead, and manganese. The concentrations of cadmium, manganese, and zinc in one group are 10, 100, and 100 μM, respectively, and the concentrations of cadmium, manganese, and zinc in another group are 50, 500, and 500 μM, respectively. Group PDA liquid culture medium without adding any heavy metal was used as a control, and each treatment concentration was repeated three times. The prepared culture medium was placed in a 200ml Erlenmeyer flask, sealed, and sterilized. After cooling, inoculate Fusarium oxysporum (Fusarium oxysporum) J-2-1-2 under aseptic conditions, culture at 25°C for 20 days, wait until the mycelium is covered with liquid medium, filter with a filter, and wash with pure water for several oxysporum (Fusarium oxysporum) J-2-1-2 mycelium, dried at 80°C to constant weight, analyzed for cadmium, lead, manganese and zinc content, the data are shown in Table 1.

[0032]Similarly, configure Hoagland's nutrient sol...

Embodiment 2

[0037] Configure a solid culture medium based on wood chips (or grass bran) plus cottonseed hulls, ammonium nitrate as an exogenous nitrogen source, sucrose as an exogenous carbon source, and potassium dihydrogen phosphate as an exogenous phosphorus and potassium source. 65%, pH 6.5, packed in polypropylene plastic bags, sealed with loops and film, and sterilized at 120°C for 90 minutes. After cooling, inoculate the solid culture medium with Fusarium oxysporum J-2-1-2 under aseptic conditions, and culture it indoors at 25°C for 30-35 days to prepare a solid microbial agent.

[0038] Take the polluted soil around the waste slag pile of the smelter, air-dry it, crush it, pass it through a 20-mesh sieve, mix it well, put it in a polypropylene plastic bag, 2kg of soil per bag, put it in an autoclave, and sterilize it at 120°C for 90 minutes , and set aside after cooling.

[0039] Put the soil in the plastic bag into a sterilized plastic pot (2kg / pot). Crush the solid bacterial a...

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Abstract

The invention provides fusarium oxysporum which is classified as fusarium. The fusarium oxysporum is preserved in the China General Microbiological Culture Collection Center on November 30, 2015, and a preservation number of the fusarium oxysporum is CGMCC 11607.Specifically, a ITS1-5 .8S-ITS2 sequence of the fusarium oxysporum is shown as SEQ ID NO:1. The invention further provides a bioremediation agent comprising the fusarium oxysporum and provides application of the fusarium oxysporum or the bioremediation agent to phytoremediation of heavy metal contaminated soil. The provided fusarium oxysporum which is a plant endophytic fungus is high in resistance to heavy metals and safe and environment friendly in remediation of the heavy metal contaminated soil.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a Fusarium oxysporum and its application in bioremediation of heavy metal-contaminated soil, in particular to a method for strengthening plant enrichment and extraction of cadmium and other heavy metals in heavy metal-contaminated soil. Background technique [0002] With the rapid development of the economy, the production and domestic wastewater, waste gas, waste residue and other wastes that have not been effectively treated have increased sharply. Many harmful substances have continuously entered the soil and accumulated. The overall situation of soil pollution is severe. Agricultural security and sustainable development have a greater impact. Among them, mining and metallurgy activities are one of the important reasons for excessive heavy metals in farmland and agricultural products. The development and utilization of mineral resources lead to heavy metal...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C12R1/77
CPCB09C1/105C12N1/145C12R2001/77
Inventor 彭克俭文涛熊如意田石强陈灿钟振宇姜苹红彭逸喆万勇
Owner 湖南省环境保护科学研究院
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