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Preparation method and application of carbon dots from beer

A technology for carbon dots and beer, applied in the field of preparation of carbon dots from beer, can solve the problems of long time required and high temperature, and achieve the effects of high safety, obvious effect, good fluorescence and biocompatibility

Inactive Publication Date: 2016-07-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the past ten years, the synthesis method of carbon dots has been greatly improved, such as hydrothermal method (document 3: B.Bourlinos, A.Stassinopoulos, D.Anglos, etal.SurfaceFunctionalizedCarbogenicQuantumDots.Small, 2008,4(4):455 -458.), oxidation method (document 4: H.P.Liu, T.Ye, C.D.Mao, Fluorescent carbonnanoparticles derived from candlesoot.Angew.Chem., Int.Ed., 2007,46(34):6473-6475.), microwave-assisted method ( Document 5: Q.L.Wang, H.Z.Zheng, Y.J.Long, L.Y.Zhang, et al. Microwave-hydrothermal synthesis of fluorescent carbon dots from graphite oxide. Carbon, 2011, 49(9), 3134-3140.), but the general synthesis conditions are cumbersome and the time required is usually longer , higher temperature, not a green synthesis method

Method used

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  • Preparation method and application of carbon dots from beer
  • Preparation method and application of carbon dots from beer
  • Preparation method and application of carbon dots from beer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of functionalized fluorescent carbon dots and loading of adriamycin

[0031] (1) Rotate about 100 mL of Tsingtao beer to about 5 mL at 60° C., filter through 0.45 μm and 0.22 μm membranes in sequence, and collect the filtrate. Subsequently, it was separated and purified by Sephadex G-25 gel chromatography, observed under the aid of 340nm excitation light, collected the strong fluorescent components, and freeze-dried to obtain carbon dots.

[0032] (2) The carbon dots and adriamycin are mixed in a mass ratio of 8:1 and dissolved in deionized water. Adjust the pH value to 8 with 1M NaOH aqueous solution, and stir for 24 hours in the dark. Add 8 times the volume of the above-mentioned mixed solution to absolute ethanol, collect the precipitate by high-speed centrifugation (10000 rpm, 10 min), and obtain carbon dots carrying adriamycin after drying.

Embodiment 2

[0033] Example 2: Characterization of beer carbon dot properties

[0034] (1) The shape and size of beer carbon dots

[0035] figure 1 It is a transmission electron microscope photograph and particle size statistical diagram of beer carbon dots. The results show that the beer carbon dots after separation and purification are uniform in size and regular in spherical shape. Statistics show that the particle size of beer carbon dots is concentrated in 2-3nm.

[0036] (2) Fluorescence spectrum and ultraviolet spectrum characteristics of beer carbon dots

[0037] figure 2 It is the fluorescence spectrum and ultraviolet spectrum of beer carbon dots. In the figure, the fluorescence spectrum of beer carbon dots shows a significant red shift as the excitation wavelength increases, and the maximum excitation wavelength of coffee carbon dots appears at 340nm. The UV spectrum shows that there is a characteristic absorption peak of n→π* transition at 270nm.

[0038] (3) Fluorescence lifetime of b...

Embodiment 3

[0049] Cytotoxicity test

[0050] Choose MCF-7 cells and high-sugar DMEM medium containing 10% fetal bovine serum, add antibiotics and non-essential amino acids. The MCF-7 cells were seeded in a 96-well plate at 10,000 cells / well, and the original medium was discarded after the adherence was complete (12h). After dissolving the carbon dots, the carbon dots carrying doxorubicin, and doxorubicin in the culture medium, they were diluted with the culture medium to different concentrations and added to the 96-well plate. After 48 hours of incubation, the growth rate was determined by MTT method. Such as Figure 8 As shown, the survival rate of MCF-7 cells can still reach more than 80% at a higher concentration of beer carbon dots (50 mg / mL), indicating that beer carbon dots are less toxic. Such as Picture 9 As shown, beer carbon dots loaded with doxorubicin have greater toxicity to MCF-7 cells.

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Abstract

The invention discloses a preparation method and application of carbon dots from beer as a main raw material. In the method, the carbon dots are separated and purified through sephadex gel chromatography and then are collected. The carbon dots can directly be used in living cell fluorescent imaging, and in addition, the carbon dots are mixed and stirred with adriamycin in water to support the adriamycin onto the carbon dots, which can be used for killing tumor cells. The beer carbon dots are low in cost, low in toxicity and simple in method, has good water solubility when the adriamycin is supported thereon, and can be used in the fields of tumor fluorescent markers and treatment as a fluorescent medicine carrier.

Description

Technical field [0001] The invention relates to a method for preparing carbon dots from beer, and its application as a fluorescent anti-tumor drug carrier in tumor cell imaging and treatment. Background technique [0002] Cancer is a major disease that seriously endangers human health. Approximately 7 million people worldwide die of cancer each year, of which 24% occur in China. There are currently three main methods of treating cancer: surgery, chemotherapy and radiation therapy. However, excessive doses of chemotherapy drugs entering the body have obvious side effects on humans. Therefore, inventing a fluorescent carrier that can carry anti-cancer drugs and effectively treat cancer without side effects is a major scientific problem of common concern to mankind. [0003] In recent years, as a new type of material, nanomaterials have attracted worldwide attention and research upsurge. And fluorescent nanomaterials have attracted much attention because of their good optical prope...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K31/704A61K47/46A61P35/00
Inventor 谭明乾王姿懿马小军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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