Method for detecting intercepted amount of bactericide in cigarette end

Abstract

The invention discloses a method for detecting an intercepted amount of bactericide in a cigarette end. The cigarette end comprises a filter tip and cigarette tobacco residues; the bactericide is one or more of carbendazim, propamocarb, isoprothiolane, triadimenol and triadimefon; the method comprises the following steps of: selecting a cigarette sample, and collecting the filter tip of the combusted cigarette; separating the filter tip from the cigarette tobaccos in the cigarette end, and respectively performing extraction on the filter tip and the cigarette tobaccos; respectively adding anhydrous magnesium sulfate, sodium chloride, sodium citrate and sodium dihydrogen citrate in sequence into a first spiral cap centrifugal pipe and a second spiral cap centrifugal pipe which are frozen under vortex oscillation; centrifugalizing after tertiary vortex oscillation is finished, and performing cryopreservation after centrifugalizing; purifying the materials through N-primary secondary amine; and finally performing analysis measurement with LC-MS / MS. According to the method, a better extraction effect is retained, and polypropylene fibers or cellulose acetate fibers in the filter tip cannot be dissolved, so that the difficulty in extracting the bactericide from an extracting solvent is reduced.

Description

technical field [0001] The invention belongs to the field of detection of pesticide residues, and relates to a detection method of bactericide interception in cigarette butts. Background technique [0002] Leaf spot and black stem are the most common bacterial diseases in tobacco, which have a great impact on the yield and treatment of tobacco. Propamocarb, carbendazim, rice blastazim, triadimenol and triadimefon are commonly used fungicides in the production of flue-cured tobacco, which can prevent and control tobacco leaf spot and black shank, and have the characteristics of high efficiency and low toxicity. Except for carbendazim, the other four indicators are included in the "China Tobacco Leaf Company's 2015 List of Pesticides Recommended for Tobacco Use" and are used in various tobacco areas. Due to their wide application, these fungicides are high residues in tobacco leaves. indicators, often detected in cured tobacco leaves. Residues of fungicides in tobacco leaves...

AI Technical Summary

Problems solved by technology

Previous researchers paid more attention to the smoke and filter transfer behavior of organophosphorus, pyrethroid and carbamate pesticides, and most of these pesticides are banned or are not recommended for use in tobacco, and the detection rate in tobacco is low

In addition, some researchers have paid attention to the smoke, ash and filter transfer behavior of maleic hydrazide, a budding agent used in tobacco. There are large differences in the transfer process of pesticides in the actual smoking process

Images