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Method for simultaneously detecting multiple sugars and sugar alcohols in food

A food and sugar alcohol technology, applied in the field of high-performance liquid chromatography detection, can solve the problems of ineffective separation of sugars, decrease of column efficiency of amino column, and decrease of column life, and achieve low machine noise, slow column efficiency drop, and column The effect of churn reduction

Inactive Publication Date: 2016-06-29
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the analysis of amino column-acetonitrile water system, as the number of detected sugars increases, the column efficiency of the amino column decreases, and some sugars cannot be effectively separated.
In order to increase the resolution, it is necessary to continuously adjust the ratio of acetonitrile to water, but when the ratio of acetonitrile in the organic phase increases to a certain extent, the effective bonding of the amino column will be destroyed, thereby reducing the column life

Method used

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  • Method for simultaneously detecting multiple sugars and sugar alcohols in food
  • Method for simultaneously detecting multiple sugars and sugar alcohols in food
  • Method for simultaneously detecting multiple sugars and sugar alcohols in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Detection of seven kinds of sugars and sugar alcohols in honey samples

[0041] (1) Preparation of seven sugar single-standard solutions: Accurately weigh sorbitol, maltitol, xylitol, fructose, glucose, sucrose, and lactose to prepare standard stock solutions, and the standard stock solution concentration of lactose is 100mg ·mL -1 , the concentration of standard stock solution of xylitol, fructose, sorbitol, glucose, sucrose is 150mg·mL -1 , the standard stock solution concentration of maltitol is 200mg·mL -1 , for use; the concentration of the standard series is shown in Table 1.

[0042]Table 1 Seven kinds of sugar standard stock solution concentration and standard series solution concentration

[0043]

[0044] (2) Preparation of standard working solution: Xylitol standard working solution 60mg·mL - : Take 150mg·mL -1 Put 4.00mL of xylitol standard stock solution into a 10mL volumetric flask, add water to the volume; fructose standard working s...

Embodiment 2

[0057] Embodiment 2: Detection of seven kinds of sugars and sugar alcohols in wine samples

[0058] Steps (1) and (2) are the same as in Example 1;

[0059] (3) Sample preparation: Mix the sample thoroughly, take 5.00-20.00g of the sample, dilute it with water, and set the volume to 50ml. After mixing, centrifuge at 6000r / min for 5min at 4°C, and take the supernatant. Filter with a 0.22 μm needle filter, and the filtrate is used for detection;

[0060] Select the liquid-phase external standard method for quantification, prepare a standard working solution according to a certain concentration, use the concentration of the standard solution and the corresponding monomer peak area signal to make a working curve, and use the formula 1 according to the response peak area and sample volume of the monomer in the sample to be tested. Calculation:

[0061] (1)

[0062] In the formula:

[0063] X—the content of the component to be tested in the sample (mg / g),

[0064] C—Calculate...

Embodiment 3

[0070] Embodiment 3: the detection of seven kinds of sugars and sugar alcohols in the liquor sample

[0071] Steps (1) and (2) are the same as in Example 1;

[0072] (3) Sample preparation: Mix the sample thoroughly, take 5.00-20.00g of the sample, dilute it with water, and set the volume to 50ml. After mixing, centrifuge at 6000r / min for 5min at 4°C, and take the supernatant. Filter with a 0.22 μm needle filter, and the filtrate is used for detection;

[0073] Select the liquid-phase external standard method for quantification, prepare a standard working solution according to a certain concentration, use the concentration of the standard solution and the corresponding monomer peak area signal to make a working curve, and use the formula 1 according to the response peak area and sample volume of the monomer in the sample to be tested. Calculation:

[0074] (1)

[0075] In the formula:

[0076] X—the content of the component to be tested in the sample (mg / g),

[0077] C—...

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Abstract

The invention relates to a method for simultaneously detecting multiple sugars and sugar alcohols in food. An HPLC-differential refraction detection method is adopted as the method; the method is characterized by comprising the HPLC chromatographic conditions that an amide-bonded column is adopted as a chromatographic column, single mobile phases of acetone, water and triethylamine are adopted as mobile phases, isocratic elution is performed, the column temperature is 70 DEG C, the flowing velocity is 0.8 ml / min, and the sample injection amount is 10 milliliters. The detection method can be suitable for the food such as honey, wine and baijiu; detection can be performed only by simply processing a sample, the method is rapid, high in recovery rate, wide in linear range, good in correlation coefficient and low in detection limit, and the blank of simultaneous detection on the sugars and sugar alcohols in the food in China is filled up.

Description

technical field [0001] The invention relates to a method for detecting sugar and sugar alcohols in foods such as honey, wine, liquor, etc., in particular to a method for simultaneously detecting sorbitol, maltitol, xylitol, fructose, glucose, sucrose and lactose in the above foods. Liquid chromatography detection method. Background technique [0002] In the current research on sugar detection methods, there are mainly high performance liquid chromatography (HPLC)-fluorescence detection (FLD), HPLC-refractive index (RID), ion chromatography, high performance liquid chromatography-mass spectrometry (HPLC- MS / MS), high performance gas chromatography-mass spectrometry (GC-MS / MS). Although the HPLC-FLD method has high sensitivity, it has strong selectivity; ion chromatography has good repeatability and accuracy, but sugars can reduce some molecules in the sample on the electrode surface; HPLC-MS / MS method Although the GC-MS / MS method has the characteristics of high separation, ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘琳张丽郝鹏飞吕宁王建华翟丽娜
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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