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Electrode and preparation method thereof, biosensor and enzyme biofuel cell

A biological enzyme and electrode technology, applied in the field of materials, can solve the problems that the output power density of enzyme biofuel cells cannot meet the actual needs, and achieve the effects of improving service life, expanding surface area, and improving stability

Active Publication Date: 2016-06-29
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are still many problems to be solved to use enzyme biofuel cells as a power source in actual production and life
Among them, the main reason restricting its practical development is that the output power density of enzyme biofuel cells is still far from meeting the actual needs.

Method used

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  • Electrode and preparation method thereof, biosensor and enzyme biofuel cell
  • Electrode and preparation method thereof, biosensor and enzyme biofuel cell
  • Electrode and preparation method thereof, biosensor and enzyme biofuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: glucose oxidase / carbon paper electrode (GOx pH＝x / CP) preparation

[0058] (1) Electrode surface treatment: drop the acid onto the surface of the carbon paper (CP) by drop coating to wet the surface of the carbon paper, and let it stand at room temperature for 5 minutes, then clean the surface of the carbon paper with a large amount of deionized water until neutral, Finally, let it dry at room temperature. The treated electrode surface as figure 2 shown.

[0059] (2) Utilize 0.2mol / L disodium hydrogen phosphate and 0.2mol / L sodium dihydrogen phosphate to prepare a phosphate (PBS) buffer solution with a concentration of 0.1mol / L and a pH of 7.2, and adjust the pH value with concentrated phosphoric acid 2, 3, 4, 5, 7 in PBS buffer.

[0060] (3) Incubate glucose oxidase (GOx) with PBS buffer solutions of different pH values, add glucose oxidase to PBS buffer solutions with different pH values, keep the temperature at 4°C, and the enzyme concentration in ...

Embodiment 2

[0063] Embodiment 2: glucose oxidase / carbon paper electrode (GOx pH＝3 / CP) performance test

[0064] The electrode preparation method is the same as in Example 1, wherein, in step (2), the pH value of the buffer solution is 3.

[0065] The electrode performance test uses a standard three-electrode system: GOx pH＝3 / CP is the working electrode, the platinum sheet is the counter electrode, and the Ag / AgCl electrode is the reference electrode. The cyclic voltammetry curve test is performed at room temperature using an electrochemical workstation CHI660E (Shanghai Chenhua, China), and high-purity argon gas is used to bubble before the test. 30 minutes. Test results such as Image 6 shown. in, Image 6 a is when the electrolyte is 0.1M PBS buffer with pH 7.2 containing 0.1M β-D-glucose, the working electrode is GOx pH＝3 Cyclic voltammogram of / CP; Image 6 b is when the electrolyte is 0.1M PBS buffer solution with a pH of 7.2, and the working electrode is GOx pH＝3 Cyclic vo...

Embodiment 3

[0066] Embodiment 3 direct electron transferase biofuel cell (anode is GOx pH＝3 / CP) battery performance:

[0067] (1) Electrode GOx pH＝3 The preparation method of / CP is the same as that of Example 2.

[0068] (2) Construction of enzyme biofuel cell: the schematic diagram of the cell structure is as follows Figure 7 shown. The battery consists of two compartments connected by a salt bridge consisting of agar and saturated potassium chloride solution.

[0069] (3) The electrolyte in the anode compartment is 0.1MPBS buffer solution containing 0.1Mβ-D-glucose, and high-purity argon gas is bubbled for 30 minutes before the battery works to remove the oxygen in the electrolyte.

[0070] (4) The cathode of the cathode chamber is 2cm 2 Platinum sheet, the electrolyte is B-R buffer solution, the pH is 3, the battery is bubbled with high-purity oxygen for 30 minutes before working, and the bubbles are continued to make the oxygen in the electrolyte saturated, and the working tem...

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Abstract

The invention discloses an electrode and a preparation method thereof, a biosensor and an enzyme biofuel cell.The electrode comprises an electrode carrier and a biological enzyme which is fixed to the electrode carrier by means of electrostatic adsorption.Through the electrode, direct electron transfer between an enzyme active center and the electrode carrier can be realized, the electrical properties of the electrode are good, and high cell power density can be obtained when the electrode is applied to the cell.

Description

technical field [0001] The invention relates to the field of materials, in particular, the invention relates to an electrode and a preparation method thereof, a biosensor and an enzyme biofuel cell. Background technique [0002] A biofuel cell is a type of battery that uses enzymes or microbial tissues as catalysts to directly convert the chemical energy of fuel into electrical energy. In addition to the advantages of high energy conversion rate and no pollution in theory, biofuel cells also have the advantages of wide range of raw materials, mild operating conditions and good biocompatibility. Biofuel cells can be divided into enzyme biofuel cells and microbial fuel cells according to their working methods. Compared with enzyme biofuel cells, microbial fuel cells have problems such as low working efficiency, relatively complicated reaction process, and difficulty in control. Therefore, enzyme biofuel cells are currently one of the research hotspots in the field of green an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/26H01M8/16H01M4/92H01M4/90H01M4/88
CPCG01N27/26G01N27/3275H01M4/88H01M4/9083H01M4/92H01M8/16Y02E60/50Y02P70/50
Inventor 胡宗倩彭瑞云焦树强康泽朋高亚兵喻超
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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