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A kind of biotransformation method of agmatine sulfate

An agmatine sulfate and biotransformation technology, which is applied in chemical instruments and methods, organic chemistry, preparation of organic compounds, etc., can solve the problems of no agmatine sulfate biotransformation method, etc., and achieves low cost and technological process. Simple, mild reaction conditions

Active Publication Date: 2019-06-21
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that there is no efficient biotransformation method for agmatine sulfate in the prior art, an embodiment of the present invention provides a biotransformation method for agmatine sulfate

Method used

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  • A kind of biotransformation method of agmatine sulfate
  • A kind of biotransformation method of agmatine sulfate
  • A kind of biotransformation method of agmatine sulfate

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Embodiment 1

[0052] Using L-arginine as the substrate, arginine decarboxylase as the biotransformation catalyst, and pyridoxal phosphate as the coenzyme, the decarboxylation reaction is carried out to generate the crude agmatine product. The crude agmatine product is diluted with dilute sulfuric acid Acidification to obtain crude agmatine sulfate. The mass ratio of arginine decarboxylase to L-arginine is 1:40; the mass ratio of pyridoxal phosphate to L-arginine is 1:1000. Control the temperature of the reaction system to 35°C; control the pH of the reaction system to 6.5.

[0053] Arginine decarboxylase is an immobilized arginine decarboxylase, and the immobilization of arginine decarboxylase includes the following steps:

[0054] 1) Crush the engineered bacterial cells containing arginine decarboxylase, centrifuge the engineered bacterial cells, and collect the arginine decarboxylase solution;

[0055] 2) Add the arginine decarboxylase solution, additives and immobilized carrier collected in s...

Embodiment 2

[0066] Using L-arginine as the substrate, arginine decarboxylase as the biotransformation catalyst, and pyridoxal phosphate as the coenzyme, the decarboxylation reaction is carried out to generate the crude agmatine product. The crude agmatine product is diluted with dilute sulfuric acid Acidification to obtain crude agmatine sulfate. The mass ratio of arginine decarboxylase to L-arginine is 1:20; the mass ratio of pyridoxal phosphate to L-arginine is 1:800. Control the temperature of the reaction system to 30°C; control the pH of the reaction system to 6.

[0067] Arginine decarboxylase is an immobilized arginine decarboxylase, and the immobilization of arginine decarboxylase includes the following steps:

[0068] 1) Crush the engineered bacterial cells containing arginine decarboxylase, centrifuge the engineered bacterial cells, and collect the arginine decarboxylase solution;

[0069] 2) Add the arginine decarboxylase solution, additives, and immobilized carrier collected in ste...

Embodiment 3

[0078] Using L-arginine as the substrate, arginine decarboxylase as the biotransformation catalyst, and pyridoxal phosphate as the coenzyme, the decarboxylation reaction is carried out to generate the crude agmatine product. The crude agmatine product is diluted with dilute sulfuric acid Acidification to obtain crude agmatine sulfate. The mass ratio of arginine decarboxylase to L-arginine is 1:30; the mass ratio of pyridoxal phosphate to L-arginine is 1:500. Control the temperature of the reaction system to 42°C; control the pH of the reaction system to 7.

[0079] Arginine decarboxylase is an immobilized arginine decarboxylase, and the immobilization of arginine decarboxylase includes the following steps:

[0080] 1) Crush the engineered bacterial cells containing arginine decarboxylase, centrifuge the engineered bacterial cells, and collect the arginine decarboxylase solution;

[0081] 2) Add the arginine decarboxylase solution, additives and immobilized carrier collected in step...

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Abstract

The invention discloses a biotransformation method of agmatine sulfate, which belongs to the field of pharmaceutical intermediates. The method comprises: using L-arginine as a substrate, using arginine decarboxylase as a biotransformation catalyst, performing a decarboxylation reaction to generate a crude agmatine product, and acidifying the crude agmatine product with dilute sulfuric acid , to obtain the crude product of agmatine sulfate. The process of the method is simple, has no special requirements for equipment, and is suitable for industrial production. Compared with the pure chemical method, the method has mild reaction conditions, easy operation, low cost, and less pollution, and can complete chemical reactions that are difficult to carry out. enzyme-catalyzed reaction.

Description

Technical field [0001] The invention relates to the field of preparation of pharmaceutical intermediates, in particular to a method for biotransformation of agmatine sulfate. Background technique [0002] Agmatine is the product of L-arginine decarboxylase (L-ADC) catalyzing the decarboxylation of arginine. It is a neurotransmitter and is distributed in most organs and tissues in mammals. Its specificity Ground exists in certain cells, such as adrenal chromaffin cells, glial cells and carotid body globular cells. Research data shows that agmatine has biological activities such as lowering blood sugar, lowering blood pressure, diuresis, anti-inflammatory, anti-depressant, and inhibiting cell proliferation, especially for N-methyl-D-aspartate (NMDA) receptors. The antagonistic effect is strong and long-lasting, and it has a withdrawal effect on morphine dependence in animals. It is a highly developed drug for detoxification. And agmatine works by stimulating the release of hormon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12N11/14C07C277/08C07C279/12
CPCC07C277/08C12N11/14C12P13/001C07C279/12
Inventor 陈建华
Owner JIANGHAN UNIVERSITY
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