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A method of extracting flavonoid compounds from tagetes erecta

A technology of flavonoids and marigolds, applied in the direction of organic chemistry, can solve the problems of poor preparation process efficiency, low sample purity, poor repeatability, etc., to achieve reduced column elution time, high degree of instrument automation, and consistency high effect

Active Publication Date: 2016-06-22
TIANJIN YAOYU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, although there have been reports on the extraction of flavonoids from marigolds, they are generally separated by traditional separation methods, and the purity of the obtained samples is low, and the efficiency of the preparation process is relatively poor.
For example, the Chinese invention patent with the publication number CN102516335A "a method for extracting flavonoids from marigold residues" adopts the traditional organic solvent extraction method, which has poor repeatability and low extraction efficiency

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  • A method of extracting flavonoid compounds from tagetes erecta
  • A method of extracting flavonoid compounds from tagetes erecta
  • A method of extracting flavonoid compounds from tagetes erecta

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Embodiment 1

[0040] The present embodiment extracts the method for flavonoid compound component from marigold, comprises the following steps:

[0041] Take dried marigold flowers as raw materials, grind them into powder, extract and rotary steam with ethanol to obtain dried marigold coarse powder, weigh 5g, dissolve in 50mL of 95% methanol-water solution, and prepare a marigold extract solution with a concentration of 100mg / mL, passed through a 0.45 μm microporous membrane, and prepared by one-dimensional liquid chromatography. One-dimensional liquid chromatography using reversed-phase column DAISOC 18 (250mm×20mmi.d., 8μm), the mobile phase is a mixture of organic phase and water phase, where A phase is water, B phase is methanol, isocratic elution method: B phase concentration 53% isocratic elution 20min . Using UV detector 210nm and 260nm as dual selective absorption wavelengths, preparation temperature is room temperature, injection volume is 500μL / needle, mobile phase flow rate is ...

Embodiment 2

[0043] The present embodiment extracts the method for flavonoid compound component from marigold, comprises the following steps:

[0044] Take dried marigold flowers as raw materials, grind them into powder, extract and rotary steam with ethanol to obtain dried marigold coarse powder, weigh 5g, dissolve in 50mL of 95% methanol-water solution, and prepare a marigold extract solution with a concentration of 100mg / mL, passed through a 0.45 μm microporous membrane, and prepared by one-dimensional liquid chromatography. One-dimensional liquid chromatography using reversed-phase column DAISOC 18 (250mm×20mmi.d., 8μm), the mobile phase is a mixture of organic phase and water phase, wherein A phase is water, B phase is methanol, isocratic elution method: B phase concentration 50% isocratic 20min. Using UV detector 210nm and 260nm as dual selective absorption wavelengths, preparation temperature is room temperature, injection volume is 500μL / needle, mobile phase flow rate is 12mL / min...

Embodiment 3

[0046] The present embodiment extracts the method for flavonoid compound component from marigold, comprises the following steps:

[0047] Take dried marigold flowers as raw materials, grind them into powder, extract and rotary steam with ethanol to obtain dried marigold coarse powder, weigh 5g, dissolve in 50mL of 95% methanol-water solution, and prepare a marigold extract solution with a concentration of 100mg / mL, passed through a 0.45 μm microporous membrane, and prepared by one-dimensional liquid chromatography. One-dimensional liquid chromatography using reversed-phase column DAISOC 18 (250mm×20mmi.d.; 8μm), the mobile phase is a mixture of organic phase and water phase, in which phase A is water, phase B is methanol, isocratic elution method: the concentration of phase B is 54% isocratic elution for 20min . The ultraviolet detector 210nm and 260nm are used as dual selective absorption wavelengths, the preparation temperature is room temperature, the injection volume is...

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Abstract

A method of extracting flavonoid compounds from tagetes erecta is provided. The method includes a step of drying tagetes erecta dry flowers, grinding into powder, dipping with ethanol or performing reflux extraction, subjecting a filtrate after filtration to rotary evaporation until a product is dry and dissolving the product into a methanol-water mixed solvent; a step of performing one-dimensional liquid chromatographic analysis, wherein DAISO C18 is adopted as a chromatographic column, a mobile phase adopting water as an A phase and methanol as a B phase is adopted, isocratic elution is performed for 20 min with the concentration of the B phase in the mobile phase being 50-55%, eluate from the 11 min to the 14 min is collected according to an ultraviolet absorption spectrum, adopted as target components, and subjected to rotary evaporation until a product is dry, and the product of the rotary evaporation is dissolved into the methanol-water mixed solution again; and a step of performing two-dimensional liquid chromatographic analysis, wherein Acchrom X-Amide is adopted as a chromatographic column, a mobile phase adopting water as an A phase and acetonitrile as a B phase is adopted, isocratic elution is performed for 40 min with the concentration of the B phase in the mobile phase being 92-98%, and eluate from the 12 min to the 17 min is collected according to an ultraviolet absorption spectrum, adopted as the target components, and subjected to rotary evaporation until a product is dry to obtain the flavonoid compounds.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, in particular to a method for extracting flavonoid components from marigolds. Background technique [0002] Flavonoids are widely found in nature and are an important class of natural organic compounds. Such compounds containing oxygen heterocycles mostly exist in higher plants and ferns, especially in all kinds of chrysanthemums. The chemical structure of flavonoids is complex and diverse, and their biological activities are diverse, which has attracted widespread attention at home and abroad, and the research progresses rapidly. The structure of the mother nucleus is as follows: [0003] [0004] Marigold, also known as Chrysanthemum chrysanthemum, scientific name Tageteserecta, belongs to the genus Marigold of Compositae, is an annual herbaceous plant, in addition to having ornamental value, it also has good medicinal value. The flavonoids contained in mar...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
Inventor 张耀洲陆洪
Owner TIANJIN YAOYU BIOLOGICAL TECH
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