Method for constructing cell transplantation slice by using human umbilical cord mesenchymal stem cells (hUC-MSCs) and amniotic membrane
A technique for stem cell and cell transplantation, which is applied in the field of human umbilical cord mesenchymal stem cells and amniotic membrane-constructed cell transplantation sheets, can solve problems such as poor effect, and achieve the effects of convenient clinical use, increased feasibility, and increased operability.
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Embodiment 1
[0034] A method of using hUC-MSCs and amnion to construct a cell transplant sheet, comprising the steps of:
[0035] (1) Take freshly collected human amniotic membrane and umbilical cord, wash and soak the umbilical cord in normal saline for later use;
[0036] (2) Amniotic membrane treatment: bluntly separate the chorion from the cleaned amniotic membrane, then wipe the amniotic membrane with gauze moistened with normal saline, and then soak the amniotic membrane in normal saline containing penicillin 150U / ml and streptomycin sulfate 150U / ml After soaking for 0.5 hours, put it into sterile glycerin and dehydrate at 4°C. The dehydration time does not exceed 12 hours, and then transfer it to sterile glycerin and store it at -20°C for later use;
[0037] (3) Cut the soaked umbilical cord into small pieces with a length of about 1-2 cm, wash, then transfer the umbilical cord tissue to a petri dish, add physiological saline to the petri dish until 1 / 2 of the umbilical cord tissue ...
Embodiment 2
[0053] A method of using hUC-MSCs and amnion to construct a cell transplant sheet, comprising the steps of:
[0054] (1) Take freshly collected human amniotic membrane and umbilical cord, wash and soak the umbilical cord in normal saline for later use;
[0055] (2) Amniotic membrane treatment: bluntly separate the chorion from the cleaned amniotic membrane, then wipe the amniotic membrane with a gauze moistened with normal saline, and then soak the amniotic membrane in normal saline containing 200 U / ml penicillin and 200 U / ml streptomycin sulfate After immersing in sterile glycerin for 1 hour, put it into sterile glycerin and dehydrate at 8°C. The dehydration time should not exceed 12 hours, then transfer it to sterile glycerin and store it at -20°C for later use;
[0056] (3) Cut the soaked umbilical cord into small pieces with a length of about 1-2 cm, wash, then transfer the umbilical cord tissue to a petri dish, add physiological saline to the petri dish until 1 / 2 of the u...
Embodiment 3
[0065] A method of using hUC-MSCs and amnion to construct a cell transplant sheet, comprising the steps of:
[0066] (1) Take freshly collected human amniotic membrane and umbilical cord, wash and soak the umbilical cord in normal saline for later use;
[0067] (2) Treatment of amniotic membrane: bluntly separate the chorion from the cleaned amniotic membrane, then wipe the amniotic membrane with gauze moistened with normal saline, and then soak the amniotic membrane in normal saline containing 50 U / ml penicillin and 50 U / ml streptomycin sulfate After soaking for 0.5-1 hour, put it into sterile glycerin and dehydrate at 2°C, the dehydration time should not exceed 12 hours, then transfer it to a sterile glycerin bottle and store it at -20°C for later use;
[0068](3) Cut the soaked umbilical cord into small pieces with a length of about 1-2 cm, wash, then transfer the umbilical cord tissue to a petri dish, add physiological saline to the petri dish until 1 / 2 of the umbilical co...
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