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Trichoderma harzianum and pythium oligadrum compounded microbial agent and application thereof

A technology of Pythium oligandrum and bacterial agent, applied in the field of compound bacterial agent composition, can solve the problems of increasing the use of pesticides, polluting groundwater resources, and short storage time, so as to improve crop quality, increase crop yield, good control effect

Inactive Publication Date: 2016-06-08
解晓燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the cultivation of crops, various soil-borne diseases such as dead seedlings, rotten roots, green withered, standing withered, yellow wilt, root rot, stem base rot, damping, etc. often plague farmers. The solution is generally to use various The excessive use of pesticides not only makes the soil harden, but also pollutes groundwater resources, resulting in the unsustainable development of agriculture, and the increased drug resistance of disease pathogens has to increase the use of pesticides, resulting in vicious circle
Trichoderma harzianum and Pythium oligandrum have their own advantages for the control of plant fungal diseases, but there is no report on the combination of Trichoderma harzianum and Pythium oligandrum for the control of soil-borne diseases
In addition, the current single-dose strains of Trichoderma harzianum and Pythium oligandrum have a short storage time, resulting in a decline in the use effect

Method used

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  • Trichoderma harzianum and pythium oligadrum compounded microbial agent and application thereof
  • Trichoderma harzianum and pythium oligadrum compounded microbial agent and application thereof
  • Trichoderma harzianum and pythium oligadrum compounded microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Maize yield increase experiment

[0033] Before corn planting, the land was deeply plowed, and the preparation of the above content was uniformly applied, and the same weight of nitrogen fertilizer was applied to the control (CK), and the yield increase rate of corn was calculated when harvesting;

[0034] Maize yield increase %: 100X (treatment mu yield-contrast mu yield) / contrast mu yield;

[0035] According to the formula of Colby's method (1966), the yield increase rate was calculated and compared with the measured yield increase rate, so as to evaluate the joint effect of the mixture simply and effectively. The calculation formula is:

[0036] E=X 1 ×X 2 …×X n / 100 (n-1)

[0037] E is the theoretical synergy of the mixture; n is the quantity of the mixture; X 1 Indicates the yield increase rate after the application of the first drug; X 2 Indicates the yield increase rate after the application of the second chemical agent; X n Indicates the yiel...

Embodiment 2

[0042] Embodiment 2 anti-dead vaccine experiment

[0043] Select the experimental field where the dead seedling disease of pepper often occurs. Before the pepper is planted, the land is deeply plowed, and the above-mentioned fertilizer is evenly applied. The control (CK) is a blank control. The pepper grows to 8-9 leaves, and the dead seedling disease of pepper is calculated. incidence rate;

[0044] Capsicum dead seedling disease inhibition rate%: 100X (control area capsicum dead seedling disease diseased strain number-treatment area capsicum dead seedling disease diseased strain number) / control area capsicum dead seedling disease diseased strain number;

[0045] According to the formula of Colby's method (1966), the inhibition rate was calculated and compared with the measured inhibition rate, so as to evaluate the joint effect of the mixture simply and effectively. The calculation formula is:

[0046] E=X 1 ×X 2 …×X n / 100 (n-1)

[0047] E is the theoretical inhibi...

Embodiment 3

[0053] Embodiment 3 Anti-bacterial wilt experiment

[0054] Select the experimental field where bitter gourd bacterial wilt often occurs, and before the bitter gourd is planted, the land is deeply plowed, and the above-mentioned fertilizer is evenly applied. The control (CK) is a blank control. incidence rate;

[0055] Bitter gourd bacterial wilt inhibition rate%: 100X (the number of bitter gourd bacterial wilt diseased plants in the control area-the number of bitter gourd bacterial wilt diseased plants in the control area) / the number of bitter gourd bacterial wilt diseased plants in the control area;

[0056] According to the formula of Colby's method (1966), the inhibition rate was calculated and compared with the measured inhibition rate, so as to evaluate the joint effect of the mixture simply and effectively. The calculation formula is:

[0057] E=X 1 ×X 2 …×X n / 100 (n-1)

[0058] E is the theoretical inhibition rate of the mixture; n is the quantity of the mixt...

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Abstract

The invention discloses a trichoderma harzianum and pythium oligadrum compounded microbial agent. The microbial agent comprises trichoderma harzianum, pythium oligadrum and a pesticide carrier. The composition is compounded from trichoderma harzianum and pythium oligadrum and can increase the crop yield, improve the soil and improve the crop quality; besides, the microbial agent has a better control effect on pathogenic bacteria of soil-borne diseases, has a remarkable synergistic interaction effect and can control seedling death, root rot, green wilting, dead standing, yellow wilt, butt rot, stalk rot, damping off and other common agricultural diseases.

Description

technical field [0001] The invention relates to a compound bacterial agent composition, in particular to a compound bacterial agent of Trichoderma harzianum and Pythium oligandrum and its application. Background technique [0002] Trichoderma harzianum is a kind of Trichoderma, the mycelium is slender and colorless, with septa, multi-branched, the conidiophores grow from the side branches of the mycelium, opposite or alternate, and the small stalks of the conidia are bottle-shaped Or cone-shaped, the conidia are mostly spherical, the spore wall has small warts, blue-green, and the colonies on the PDA medium are white flocculent at first, and then dark green. As a biocontrol fungus, Trichoderma harzianum can be used to prevent plant diseases caused by pathogens such as Pythium, Rhizoctonia solani, Fusarium, Rhizopus niger, Cylindrosporium, Sclerotinia, and Sclerotinia. Trichoderma harzianum can grow rapidly around the roots and leaves of plants, occupying the sites on the su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00A01P3/00
CPCA01N63/30A01N2300/00
Inventor 解晓燕
Owner 解晓燕
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