High performance liquid chromatography and Fourier transform infrared spectroscopy combined interface device

Abstract

The invention discloses a high performance liquid chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR) combined interface device, and belongs to the field of environmental studies. The device is composed of seven components: an optical table, a zinc selenide (ZnSe) crystal, a round drive rotary table, a heating casing pipe, an atomizer, a cleaning device and a drying device. A target pollutant enters a heating casing pipe and atomizer integrated device after being separated by a chromatography column, is heated and atomized, and then is evenly sprayed on the surface of the ZnSe crystal, and the ZnSe crystal is rotated to the optical table from the round drive rotary table. Infrared light enters the ZnSe crystal at a certain angle through the optical table, is reflected, then enters an FTIR detector and is subjected to atlas analysis. The ZnSe crystal having testing completed is transferred to the cleaning device and the drying device successively, is washed and dried respectively and then is re-used. The round rotary table carries the crystal and a sample for reciprocating detection by means of rotation, and sample batch processing and on-line detection are realized.

Description

Technical field: [0001] The invention relates to an interface device for high-performance liquid chromatography and Fourier transform infrared spectroscopy, in particular to realize the successful separation and qualitative analysis of effective detection of pollutants in water bodies. Background technique: [0002] Currently, gas chromatography and liquid chromatography are widely used effective methods for separation, analysis and purification of organic compounds. Among the known organic compounds, about 80% of them have high boiling points and are difficult to vaporize and volatilize, so gas chromatography cannot effectively separate them. High Performance Liquid Chromatography (High Performance Liquid Chromatography, HPLC) is an effective means of separating difficult volatile complex substances due to its mild conditions and no damage to samples. However, conventional chromatographic detection techniques are not effective in structure elucidation and isomer identifica...

AI Technical Summary

Problems solved by technology

Since the mobile phases used in HPLC are often organic solvents, they often have their own characteristic frequency absorption bands in the infrared region, which seriously affects the infrared spectrum identification of chromatographic fractions.

At present, some progress has been made in the development of HPLC-FTIR interface, but there are still some problems. For example, the solvent needs to be heated, which is very unfavorable for the measurement of thermally decomposed compounds; due to the large energy loss of reflection spectroscopy, it has a great impact on the detection sensitivity. ;Complicated mechanical system, difficult processing and manufacturing, etc.

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