Para hydroxybenzene alanine urine detection kit and preparation method thereof

Abstract

The invention provides a para hydroxybenzene alanine urine detection kit. The para hydroxybenzene alanine urine detection kit comprises a reagent A, a reagent B, a reagent C and a reagent D which are stored in four containers respectively. The reagent A is an inorganic acid water solution with the concentration of 0.01-0.5 mol / L. The reagent B is a sodium nitrite water solution with the concentration of 0.01-0.5 mol / L. The reagent C is an inorganic acid water solution of an aniline derivative with the concentration of 0.005-0.1 mol / L, the concentration of inorganic acid ranges from 0.01-0.5 mol / L, and a benzene ring of the aniline derivative at least comprises a sulfo group or a nitro group or a cyano group or a fluorine group. The reagent D is a water solution of N-1-naphthyl ethylenediamine dihydrochloride, and the concentration of N-1-naphthyl ethylenediamine dihydrochloride is 0.01-0.3 mol / L. The concentration of the aniline derivative in the reagent C is not higher than that of inorganic acid in the reagent A and that of sodium nitrite in the reagent B. Inorganic acid is selected from hydrochloric acid or sulfuric acid or tetrafluoroboric acid or hexafluorophosphoric acid. The invention further provides a preparation method of the kit. The detection kit has the good stability, reliability and broad spectrum.

Description

technical field [0001] The invention relates to an in vitro detection kit for clinical diagnosis, in particular to a p-hydroxyphenylalanine urine detection kit and a preparation method. Background technique [0002] Cancer mortality ranks first among all diseases and is the number one killer of human health. It has become a major cause of death that threatens human life and health. According to statistics, the number of cancer patients will double every 25 years. Not only that, the number of deaths from cancer is also rising rapidly around the world, and this number may increase to 13.2 million in 2030. [0003] Early detection is the key to the treatment of tumors. The development of tumors is divided into five stages: benign, tumor cell initiation, early tumor, mid-tumor, and late stage. The frightening thing about cancer is that the first three stages cannot be detected by imaging. Even in the middle stage of the tumor, only very professional doctors can detect it with a...

AI Technical Summary

Problems solved by technology

For example, firstly, the monohydric phenol in the urine first reacts with the mercury salt in the reagent A to form a complex, and this complex is due to the hydroxyl group in the monohydric phenol donating its lone pair of electrons to the empty orbital of the metal ion, As a result, the ability of the hydroxyl group to donate electrons to the aromatic ring decreases, and the complexed structure reduces the electron cloud density on the aromatic ring, which eventually leads to insufficient activity in the subsequent diazotization reaction and ultimately leads to inaccurate detection; in addition, reagent B The nitrous acid in the solution is easy to decompose during storage and transportation, which makes it unable to react with reagent C to form diazonium salt, which affects the accuracy of detection

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