Gene SbAn-1 capable of controlling existence of sorghum awn and SNP mutation site and application

A technology of sban-1 and sorghum awn, applied in the direction of DNA / RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problem of not having cloned genes

Inactive Publication Date: 2016-05-25
LIAONING ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fine mapping of the sorghum awn gene is rarely reported at home and abroad. In 2014, Zhai Guowei and others fine-mapped the 115Kb range of the third chromosome, but no gene was cloned.

Method used

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  • Gene SbAn-1 capable of controlling existence of sorghum awn and SNP mutation site and application
  • Gene SbAn-1 capable of controlling existence of sorghum awn and SNP mutation site and application
  • Gene SbAn-1 capable of controlling existence of sorghum awn and SNP mutation site and application

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Embodiment 1

[0029] The gene SbAn-1, which controls the presence or absence of sorghum awns, is viewed from the BTx623 reference genome. When there is no awn, it is the normal sequence of the gene Sobic.003G417700, while there are three mutations in the gene of awned sorghum, the first point is 72367561, The mutation of T to C, that is, the mutation of TTT (phenylalanine) to TTC (phenylalanine), is a synonymous mutation, and non-synonymous mutations occurred at the other two sites. The non-synonymous mutation SNP sites are located at nucleotides 72367415 and 72367368 on chromosome 3 of the sorghum genome. The nucleotides at these two sites were mutated from G to A, C to T, and the amino acids were mutated from Arginine (Arg / R) was mutated to histidine (His / H) and cysteine ​​(Cys / C), and the nucleotide sequence amplified from sorghum genomic DNA by the primer pair Sb03g045130F and Sb03g045130R was subjected to After electrophoresis detection and sequencing, the presence or absence of sorghu...

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Abstract

The invention discloses a gene SbAn-1 with the function of controlling existence of sorghum awn and belongs to the field of molecular biology and sorghum breeding. The gene SbAn-1 capable of controlling existence of sorghum awn is obtained through genome-wide association study. According to the technology, a plurality of nucleotide polymorphic sites can be detected at a time, and therefore large-batch excavation and simple trait genes are facilitated. The gene is selected on the basis of the PCR technology, and the technology can be directly used for identifying the gene SbAn-1 with the function of controlling existence of sorghum awn and allelic genes thereof, so that molecular marker assisted breeding is achieved.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to the gene SbAn-1 for protecting and controlling sorghum with or without awn and the application of the gene, and belongs to the fields of molecular biology and sorghum breeding. Background technique [0002] Sorghum (Sorghumbicolor (L.) Moench) belongs to the genus Sorghum of the monocotyledon class Poaceae. It is an annual tall herb with a waxy epidermis and a typical C. 4 Plants have specialized photosynthetic organs, high photosynthetic efficiency and high yield potential. They are resistant to drought, waterlogging, barrenness, and salinity. Rice, barley), mainly distributed in arid and semi-arid regions of Africa, Asia, and America, is one of the oldest cultivated crops in the world. In addition to being used as food and feed, the main product of sorghum, grains, can also be used to make wine, starch, vinegar, and maltose. Because the phenotypic characteristics of the panic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 陆晓春白春明刘轶飞王春语丛玲朱振兴王平张丽霞李丹郑文静李金红陶承光邹剑秋
Owner LIAONING ACAD OF AGRI SCI
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