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Preparation method of high-content sisomicin fermentation liquor

A technology of sisomycin and fermented liquid, which is applied in the field of preparation of high-content sisomycin fermented liquid, can solve the problems of low biological potency, high purification yield, and large difference between high and low, and achieve high biological potency, High purification yield and easy operation

Inactive Publication Date: 2016-05-25
CHANGZHOU LANXU CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: aiming at the low biological potency of the current sisomycin fermented liquid, large disparity in height, unstable production, low main component of sisomycin, and low extraction and purification yield, The present invention first activates Micromonospora inyoi, then induces variation of the activated strains in ethyl methanesulfonate and microwave state, then puts the mutant strains into a fermenter, and dissolves them in a cobalt chloride solution Promote the production of sisomycin, and at the same time, produce sisomycin under the trigger of the bacterial liquid, and then separate it by filtration to prepare a high-content sisomycin fermentation broth with high purification yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] In terms of percentage by weight, take 30 parts of sucrose, 25 parts of agar, 15 parts of potassium nitrate solution with a mass fraction of 10%, 12 parts of a sodium chloride solution with a mass fraction of 12%, and 18 parts of glucose. Adjust the pH to 7.0 with a 5% sodium hydroxide solution, and sterilize at 120°C for 30 minutes to obtain a slant medium; use an inoculation loop to inoculate Micromonospora innio into the above slant medium, and inoculate once. Place the inoculated slant culture medium at 32°C in a sterile environment, and after cultivating for 7 days, move the culture medium to a refrigerator at 0°C for storage for 4 days to obtain the cultured strains on the slant for future use; parts by weight , get 35 parts of starch, 18 parts of bean curd residue, 32 parts of beef extract, 4 parts of L-proline, 10 parts of magnesium sulfate solution with a mass fraction of 10%, 1.2 parts of dipotassium hydrogen phosphate and 0.8 part of potassium dihydrogen phosp...

example 2

[0022]In terms of percentage by weight, take 35 parts of sucrose, 22 parts of agar, 13 parts of potassium nitrate solution with a mass fraction of 10%, 10 parts of a sodium chloride solution with a mass fraction of 12% and 20 parts of glucose. Adjust the pH to 7.0 with a sodium hydroxide solution with a fraction of 5%, and sterilize at 130°C for 40 minutes to obtain a slant medium; use an inoculation loop to inoculate Micromonospora ineosus into the above slant medium, inoculate 3 times, Place the inoculated slant medium at 35°C in a sterile environment, and after culturing for 9 days, move the medium to a refrigerator at 4°C and store it for 6 days to obtain the cultured strains on the slant for future use; parts by weight , get 42 parts of starch, 15 parts of bean curd residue, 30 parts of beef extract, 3 parts of L-proline, 8 parts of magnesium sulfate solution with a mass fraction of 10%, 1.2 parts of dipotassium hydrogen phosphate and 0.8 part of potassium dihydrogen phosp...

example 3

[0025] In terms of percentage by weight, take 34 parts of sucrose, 24 parts of agar, 13 parts of potassium nitrate solution with a mass fraction of 10%, 11 parts of a sodium chloride solution with a mass fraction of 12%, and 18 parts of glucose. Adjust the pH to 7.0 with a sodium hydroxide solution with a fraction of 5%, and sterilize at 130°C for 35 minutes to obtain a slant medium; use an inoculation loop to inoculate Micromonospora ineosus into the above slant medium, inoculate twice, Place the inoculated slant culture medium at 35°C in a sterile environment. After cultivating for 8 days, move the culture medium to a refrigerator at 2°C and store it for 5 days to obtain the cultured strains on the slant for future use; parts by weight , get 40 parts of starch, 17 parts of bean curd residue, 31 parts of beef extract, 3 parts of L-proline, 8 parts of magnesium sulfate solution with a mass fraction of 10%, 0.6 part of dipotassium hydrogen phosphate and 0.4 part of potassium dih...

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Abstract

The invention discloses a preparation method of high-content sisomicin fermentation liquor, which belongs to the field of the medicine synthesis, and aims at solving the problems of the existing sisomicin fermentation liquor that the biological value is relatively low, the difference between a maximum value and a minimum value is great, the production is unstable, the content of a main component of the sisomicin is not high and the extraction and purification yield is low. The method comprises the steps: performing the activation by virtue of micromonospora inyoensis, then performing induced variation for an activated bacterial strain under the condition of ethyl methanesulfonate and microwaves, placing the mutated bacterial strain in a fermentation tank, promoting generation of sisomicin in a cobalt chloride solution, generating the sisomicin under the initiation of a specific bacterial liquid, and then filtering and separating to obtain the high-content sisomicin fermentation liquor. The purification yield is high.

Description

technical field [0001] The invention discloses a preparation method of a high-content sisomycin fermented liquid, which belongs to the field of drug synthesis. Background technique [0002] Aminoglycoside antibiotics are a class of antibiotics discovered earlier after penicillin. Sisomicin (SISO) is an aminoglycoside antibiotic biosynthesized by Micromonospora. raw material. Both sisomicin and netilmicin are included in the National List of Essential Medicines in China, and have strong antibacterial effects against most Gram-positive and Gram-negative bacteria. [0003] The existing method for producing sisomycin fermented liquid is to use cornstarch, soybean cake powder, etc. as raw materials, and under the action of microorganism Micromonospora inyoi, three-stage fermentation is mostly used, and timely fermentation is carried out during the fermentation process. The sisomycin fermented liquid was produced by supplementing materials, and the sisomycin titer was determine...

Claims

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Application Information

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IPC IPC(8): C12P19/48C12R1/385
CPCC12P19/485
Inventor 汪巍宋国
Owner CHANGZHOU LANXU CHEM CO LTD
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