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Application of Atractylodes lactone in the preparation of antidepressants

An atractylodes lactone, antidepressant technology, applied in the directions of drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve the effects of good protection activity, shortening immobility time, and easy operation

Active Publication Date: 2019-02-26
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the application of Atractylodes lactones in the preparation of drugs for the prevention or treatment of depression.

Method used

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  • Application of Atractylodes lactone in the preparation of antidepressants
  • Application of Atractylodes lactone in the preparation of antidepressants
  • Application of Atractylodes lactone in the preparation of antidepressants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Study on the protective effect of Atractylodes macrolides on PC12 cells damaged by corticosterone

[0021] 1.1 Cell culture

[0022] PC12 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. The culture system used RPMI-1640 medium containing 5% fetal bovine serum and 10% horse serum at 37°C, 5% CO 2 cultured in a cell culture incubator. Coat culture flasks and culture dishes with poly-L-lysine (0.01mg / m1) before culturing, and change the medium every 3 days.

[0023] 1.2 Experimental method

[0024] 1.2.1 MTT method to detect the influence of atractylodes lactones I and III on the survival rate of corticosterone-injured PC12 cells

[0025] The present invention adopts MTT method to evaluate the protective activity of atractylodes lactones I and III on PC12 cells damaged by corticosterone. After PC12 cells were digested with trypsin, the number of cells per milliliter was 2×10 4 The suspension was inoculated in a 96-well cu...

Embodiment 2

[0039] Example 2 Antidepressant in vivo pharmacodynamics experiment of Atractylodes macrolides

[0040] 1.1 Experimental materials and grouping

[0041] Male ICR mice, weighing 18-20 g, were provided by Beijing Weitong Lihua Animal Experiment Center. Each 10 birds / cage was raised, free to eat and drink, room temperature (22±2)°C, natural light. Normal mice were randomly divided into 8 groups according to body weight, 12 in every group, i.e. blank control group, positive drug fluoxetine group (5mg / kg), atractylodes lactone I high (30mg / kg), middle (15mg / kg) , low (7.5mg / kg) dosage group and high (30mg / kg), middle (15mg / kg), low (7.5mg / kg) dosage group of Atractylodes lactone III. Each group was administered intragastrically at 0.2mL / 10g body weight for 7 days.

[0042] 1.2 Experimental method

[0043] 1.2.1 Mouse tail suspension test

[0044] After 1 hour of administration on the 7th day, the tail end of a single mouse (2 cm away from the tail end) was glued to the upper p...

Embodiment 3

[0057] Embodiment 3. the preparation of Atractylodes lactone I, III tablet

[0058] Heat and melt 80g polyethylene glycol 4000, add 20g atractylodes lactone I (or atractylodes lactone III) while stirring, mix and cool, crush the solidified product obtained after cooling, add 185g starch, mix well to make granules, add Mix 15 g of sodium carboxymethyl starch and an appropriate amount of magnesium stearate, and press into tablets to obtain Atractylodes lactone I (or Atractylodes lactone III) tablet.

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Abstract

The invention provides application of atractylenolide in preparation of an antidepressant drug, and relates to the field of medicine. In-vitro activity tests prove that atractylenolide I and atractylenolide III have the better protection activity on corticosterone-damaged PC12 cells, and the mechanism is related to inhibition of neuron apoptosis; in-vivo test results show that atractylenolide I and atractylenolide III can significantly shorten the immobility time of a mouse forced swimming test and a mouse tail suspension test. Experiments prove that atractylenolide I and atractylenolide III have the significant antidepressant effect and can be used for preparing the drug for preventing and treating a depressive disorder.

Description

Technical field: [0001] The present invention relates to the application of atractylodes lactone, in particular to the application of atractylodes lactone in the preparation of antidepressant drugs. Background technique: [0002] Depression is characterized by persistent low mood and cognitive dysfunction, including restlessness, anhedonia, sleep disturbance, guilt and repeated suicidal thoughts, etc. Disabling rate and high fatality rate and so on. The World Health Organization reports that more than 350 million people worldwide suffer from depression, and by 2020 depression may become the second leading disease after heart disease. With the rapid development of social economy, the pace of life is accelerated, people's pressure increases, and various emotional impacts increase, resulting in an increase in the incidence of depression year by year. Depression is called "the epidemic of the 21st century", and its harm has attracted more and more attention from the medical an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61P25/24
CPCA61K9/1652A61K9/2059A61K31/365
Inventor 宫文霞周玉枝秦雪梅张丽增杜冠华
Owner SHANXI UNIV
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