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Preparation method of hollow mesoporous silica loaded by nanometer star-shaped gold particles

A technology of mesoporous silica and nano-venus, which is applied to the preparation of medical preparations containing active ingredients, pharmaceutical formulas, X-ray contrast agents, etc., can solve the problems of preparation and application of multifunctional nanoparticles that have not been found

Inactive Publication Date: 2016-05-11
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A search of domestic and foreign literature and patents on multi-modal imaging and therapeutic integrated nano-platforms shows that no US / CT / PA multi-modal imaging and photothermal therapy based on nano-venus-loaded hollow mesoporous silica have been found so far. Report on the preparation and application of integrated multifunctional nanoparticles

Method used

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  • Preparation method of hollow mesoporous silica loaded by nanometer star-shaped gold particles
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  • Preparation method of hollow mesoporous silica loaded by nanometer star-shaped gold particles

Examples

Experimental program
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Effect test

Embodiment 1

[0076] The specific process of preparing HAPP nanoparticles is as follows: figure 1 shown.

[0077] (1) Mix 71.0mL of absolute ethanol, 10.0mL of ultrapure water and 3.14mL of ammonia water at room temperature, and stir in a 30°C water bath for 30min, then quickly add 6mL of tetraethyl orthosilicate (TEOS), and continue to stir for 1h. Solid silica particles (sSiO 2 ), then quickly add pre-mixed 5mLTEOS and 2mL carbooctadecyltrimethylsilane (C18TMS) mixture, continue to stir for 1h, and collect by centrifugation at 8500r / min to obtain solid silicon particles wrapped in mesoporous silicon (mSiO 2 sO 2 ). TEM test results show that: mSiO 2 sO 2 The core is solid silicon with a diameter of about 150nm, and the outer shell is mesoporous silicon with a thickness of about 30nm ( figure 2 a 1 -a 3 ).

[0078] (2) Divide the product obtained in (1) into 8 parts, and disperse them in 40mL anhydrous sodium carbonate solution (0.7M) respectively (dividing into 8 parts can make ...

Embodiment 2

[0085] The product obtained in (6) in Example 1 was configured into a 4 mg / mL mother solution with sterile PBS buffer, and then serially diluted to 2, 1, 0.5 and 0.25 mg / mL of the material. The cultured C6 cells were seeded in a 96-well plate, and inoculated at a density of 8,000 cells / well, with a volume of 100 μL per well. After culturing overnight, the above-mentioned materials of each dilution gradient were added and co-cultured with the cells for 24 hours. Each gradient was diluted 10-fold with culture medium, that is, the final concentrations in each well were 400, 200, 100, 50, 25 and 10 μg / mL. Five parallel wells were made for each gradient, and PBS buffer was used as a blank control. After the culture, wash with 100μL PBS for 3 times. One group of experiments was irradiated for 30s under ultrasonic (1MHz-1.2W / cm2, duty ratio: 50%), and the other experiment was not irradiated with ultrasonic. After that, each well of the two groups of experiments Add 100 μL serum-fre...

Embodiment 3

[0087] Get the obtained HAPP particles in (6) in Example 1 and the obtained HAP in (1) in Comparative Example 1 to be formulated with ultrapure water into materials with a concentration of 20 mg / mL and 5 mg / mL, fill the PE tubes of 2 mL respectively, and treat the two A group of materials were subjected to contrast mode ultrasound imaging test. Ultrapure water was used as blank control. The test results showed that within the range of the test concentration, the HAPP particles coated with HPF showed a better US imaging effect than the HAP particles ( Figure 7 ).

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Abstract

The invention relates to a preparation method of hollow mesoporous silica loaded by nanometer star-shaped gold particles. The method includes the steps of forming solid silicon wrapped by meso-porous silicon in an ethyl alcohol, ultrapure water and ammonia mixed solution with ethyl orthosilicate as a silicon source, etching the solid silicon through sodium carbonate to form hollow mesoporous silica, modifying the surface of the hollow mesoporous silica by sulfydryl and loading the surface of the hollow mesoporous silica with nanometer gold particles so that the hollow mesoporous silica can serve as seeds, making the seeds grow into the hollow mesoporous silica loaded by nanometer star-shaped gold particles in a chloroauric acid solution, and wrapping perfluorohexane and modifying polyethylene glycol with one end being -SH. The prepared nanometer particles have excellent biocompatibility and long in-vivo blood circulation time, have good US / CT / PA imaging and photo-thermal treatment effects and are wide in application prospects, and a new method is provided for development of multi-mode imaging contrast media and treatment integrated platforms.

Description

technical field [0001] The invention belongs to the field of hollow mesoporous silica, in particular to a method for preparing hollow mesoporous silica supported by nano-venus. Background technique [0002] Common molecular imaging methods include ultrasound imaging (Ultrasound Imaging, US), X-ray tomography (X-ray computedtomography imaging, CT) and photoacoustic imaging (Photoacoustic Imaging, PA) and so on. Ultrasound imaging has continuous dynamic imaging, but its resolution and sensitivity are low; CT imaging has high resolution and sensitivity, but there are many reconstruction image artifacts; photoacoustic imaging has good anti-interference, but deep tissue imaging is greatly affected by absorption . These three imaging modes have their own advantages and disadvantages. If multiple imaging modes are combined and complement each other for comprehensive diagnostic analysis, richer and more accurate image information can be obtained. Therefore, the development of mult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/04A61K49/22A61K41/00A61P35/00
CPCA61K41/0052A61K49/0002A61K49/04A61K49/22
Inventor 史向阳李鑫许晓迎罗宇沈明武刑玲溪赵凌舟杜联芳
Owner DONGHUA UNIV
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