Quick detection method of carotenoid content in marine bivalve organism

Abstract

The invention discloses a quick detection method of a carotenoid content in a marine bivalve organism. The method comprises the following steps: (1) sampling and pretreatment of tissues; (2) tissue slicing and chromatic aberration detection; (3) total carotenoid content (TCC) detection; (4) establishment of a chromatic-aberration-TCC regression curve; and (5) total carotenoid content (TCC) detection of an adductor muscle of a to-be-detected marine bivalve organism. According to the method, the adductor muscle tissue of the marine bivalve organism is sliced and is subjected to chromatic aberration detection, and chromatic aberration is associated with the carotenoid content to establish a chromatic-aberration-TCC equation of linear regression. Therefore, the carotenoid content detection of the organism is detected through a corresponding chromatic aberration parameter. The method is free of extra sample consumption, has good repeatability and practicability, and is quick and efficient.

Description

technical field [0001] The invention belongs to the technical field of carotenoid content detection, in particular to a method for rapidly detecting carotenoid content in marine bivalve organisms. Background technique [0002] Carotenoids (CAR), as a polyene substance, has certain antioxidant capacity due to its polyunsaturated double bonds, and also has other biological functions. Studies have shown that the carotenoid content in shellfish has a certain correlation with the growth and life of shellfish and gonad development. For example, the study of Patinopectenyessoensis showed that the content of carotenoids in adductor muscle reaches the highest value during the gonad development period, and the content of carotenoids decreases after the reproductive period; The survival rate during the cage culture process and after spawning was higher than that of the control group, and it had significantly higher stress resistance; while the study of Chlamysnobilis Reeve found that ...

AI Technical Summary

Problems solved by technology

However, to date, there are few studies on large-scale, population-level rapid detection methods for CAR content in these species

Existing determination method is carried out by adopting the process and method of drying, extracting and spectrophotometric detection, but this conventional method has some disadvantages, such as: 1, time-consuming

The freeze-drying process of samples generally takes 2 to 3 days to complete and the number of samples processed each time is limited. The grinding, weighing, extraction and testing of samples are also labor-intensive and time-consuming, and the work intensity is relatively high; 2. The amount of samples used is large

For the sake of ensuring accuracy, the amount of dry samples needed each time is 0.1-0.2g, which consumes a lot of experimental samples; 3. Involving volatile drugs

The sample needs to use acetone during the extraction process. The drug is volatile at room temperature and is flammable, so it has a certain impact on the laboratory environment and safety; 4. Weak practicability

Only a small amount of samples can be measured each time, and it cannot be measured in batches, so the practicability is weak

This conventional method for determining the total carotenoids content (Totalcarotenoidscontents, TCC) is far from being applicable to the needs of large-scale, multi-batch scientific research on bivalve molluscs. Therefore, a fast, efficient and reliable method must be established. Method for Determining TCC

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