Crofton weed extract and iprodione compound fungicide
A technology of Erythrina japonica and Isoflumuron, which is applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of incomplete control effect, many application times, and large amount of Isoflumuron, so as to achieve the goal of prevention and control. Plant fungal disease, increase the use effect, the effect of fast degradation
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Embodiment 1
[0015] Example 1 Extraction of Eupatorium adenophorum Extract
[0016] The collected leaves of Eupatorium adenophorum were dried in the shade and pulverized into a coarse powder, adding methanol 6 times the weight of the coarse powder, ultrasonically extracting at room temperature three times, 12 hours each time, combining the filtrates, concentrating under reduced pressure at 45°C to obtain an extract. Add a small amount of methanol to the extract to dissolve, add distilled water to dilute the methanol content to 20%, extract with ethyl acetate, recover the solvent under reduced pressure at 45°C from the extract to dryness, and obtain the ethyl acetate extract. The ethyl acetate extract was dissolved in a mixed solution of methanol:water:chloroform (85:15:5), and eluted on an XAD-2 macroporous resin column to remove impurities such as pigments, and the eluent was collected and concentrated to obtain a paste. The paste after decolorization was subjected to repeated silica ge...
Embodiment 2
[0017] Embodiment 2 antibacterial experiment
[0018] For the test bacteria: Pythium ingross.
[0019] Test agent: iprodione (FMC Corporation, USA), and zelenone was prepared by the method in Example 1.
[0020] The extract of Eupatorium adenophorum and the combined fungicide containing the extract of Eupatorium adenophorum were tested for in vitro biological activity of the tested pathogenic bacteria by the method of mycelial growth rate. Calculate the bacteriostatic rate by following formula: Bacterial colony growth diameter (mm)=two times measure diameter average value-4.0 (bacteria cake diameter); Inhibition growth rate=(the diameter of control bacterium colony-the diameter of processing bacterium colony) / the diameter *100 of control bacterium colony %.
[0021] According to the formula of Colby's method (1966) to calculate the control effect and compare with the measured control effect, it is simple and effective to evaluate the combined effect of the mixture. The ca...
Embodiment 3
[0028] Embodiment 3 is to the control field experiment of Pythium ingross
[0029] Pharmaceutical composition 1 was prepared. Composition 1 contained 50 mg / L iprodione, 50 mg / L zelenone, 50 mg / L xylene and 50 mg / L dimethyl sulfoxide, and the rest was water.
[0030] Prepare pharmaceutical composition 2, which contains 50 mg / L iprodione, 50 mg / L zelenone, 50 mg / L xylene and 50 mg / L polyoxyethylene sorbitan fatty acid ester, and the rest is water.
[0031] 200m from the peanut field 2 , Infected with Pythium massifera and fell ill, moderately infected, divided into two pieces on average, applied the pesticides of medicament composition 1 and medicament composition 2, and watered 1L each time (dilute 1L pesticide into 1000L water for watering), every other day After watering once and watering 3 times, after 15 days, the recovery rate of the peanuts with the application of the pharmaceutical composition 1 was 85%, and the recovery rate of the peanuts with the application of the...
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