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Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain

A technology for plant diseases and chaetomes, applied in the direction of microorganisms, fungi, fungicides, etc., can solve the problems of biological characteristics of bio-control bacteria and main resistance research lag, narrow antibacterial spectrum, etc.

Inactive Publication Date: 2016-04-27
WEIFANG UNIVERSITY
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Problems solved by technology

The reason is that in addition to the fact that the microbial active ingredients of bio-control bacteria are easily affected by environmental factors during production, storage and application, most of the bio-control bacteria reported so far only act on one or a certain type of plant diseases. Narrow, and the research on the main biological characteristics and main resistance and tolerance of isolated and screened biocontrol bacteria lags behind

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  • Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain
  • Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain
  • Broad-spectrum chaetomium strain applied to biological control of plant diseases and applications of broad-spectrum chaetomium strain

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Embodiment 1

[0016] The screening of embodiment 1 bacterial strain

[0017] The biocontrol strain was isolated and screened from the scale leaf twigs of Cypress cypress by tissue separation method. Firstly, tap water was used to remove the dust on the branchlets of healthy Cypress scale leaves, 75% ethanol, 0.1% mercury liter were disinfected in turn, and then rinsed with sterile water 5 times. Cut out 2-3mm long sterilized cypress scale leaf sprigs and culture them on PDA plates at 25°C and 12 hours of light. The dominant strains obtained were purified on PDA plates again, and the morphological characteristics of the colonies were observed and recorded.

[0018] Identification of bacterial species: ① Morphological observation: use 1% glucose solution to make the isolated and purified colony into a bacterial suspension and temporarily install it, and observe the morphological characteristics of the pathogenic bacteria with the help of a digital microscope; ② ITS sequence analysis: the geno...

Embodiment 2

[0023] Antibacterial experiment of embodiment 2 bacterial strains

[0024] (1) Experiments on the inhibitory effect of the strains of the present invention on pathogenic Fusarium oxysporum and Alternaria spp.

[0025] Use the plate confrontation culture method to screen biocontrol bacteria and detect the antibacterial rate: inoculate the isolated and purified LB-1 strain with a diameter of 6mm and the indicator bacteria cake on the relative positions of the two ends of the PDA medium, and place them in a light incubator for cultivation (25°C, 8h light). The indicator bacteria cultured alone were used as the control. After 3 days, use the cross method to measure the colony radius (Rc) cultured alone in the control plate and the tendency radius (Rp) in the confrontation culture plate, calculate the inhibition rate according to the following formula, and analyze the effect of the screened biocontrol bacteria on the indicator bacteria Inhibition (Deng Zhenshan et al., 2009): Inh...

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Abstract

The invention provides a broad-spectrum chaetomium strain applied to biological control of plant diseases. The broad-spectrum chaetomium strain is separated and screened from scale-leaf branches of sabina chinensis; through the morphological observation and the ITS sequence analysis, the broad-spectrum chaetomium strain is identified to be C.subaffine in the Chaetomium spp fungus, the broad-spectrum chaetomium strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on Oct 19, 2015, and the preservation number of the broad-spectrum chaetomium strain is CGMCC No.11400. The broad-spectrum chaetomium strain can be applied to the biological control of plant diseases, has good inhibiting effects for various common phytopathogens (wilt fusarium oxysporum, botrytis cinerea and root rot fungus) in production, and particularly has obvious inhibiting effects for intractable soil-borne plant pathogens (verticillium dahliae and fusarium oxysporum), the successive cropping obstacle caused by root diseases under the planting mode of the facility can be overcome, and the high-quality and pollution-free development of the agricultural industry is promoted.

Description

technical field [0001] The invention relates to a Chaetomium strain, which belongs to the field of biological control in plant protection and comprehensive management of plant diseases. Background technique [0002] Biological control refers to the plant disease control technology that uses beneficial organisms and their products to inhibit the survival and activity of pathogens, thereby reducing or inhibiting the occurrence and development of diseases. Compared with chemical control, biological control has the advantages of being safe for humans and animals, not inducing pathogenic bacteria to develop drug resistance, and playing a positive and long-term role in regulating the ecological environment. Therefore, with the enhancement of people's awareness of environmental protection, it has become a new trend in the development of modern agricultural industrialization to vigorously develop and apply biological pesticides to gradually replace chemical pesticides. [0003] Bio...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/645
Inventor 刘彩云常志隆阚世红
Owner WEIFANG UNIVERSITY
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