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Compound ethanol enzyme with acid proteases and method for preparing compound ethanol enzyme

A technology of acid protease and alcohol compound enzyme, which is applied in the field of alcohol compound enzyme and its preparation, can solve the problems of low antioxidant capacity, large loss of enzyme activity, low activity of protease, etc., and achieve the goal of ensuring food safety and improving the utilization rate of raw materials Effect

Inactive Publication Date: 2016-04-27
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem solved by the present invention is to overcome the defects of low protease activity, incomplete complex enzyme enzyme system, low antioxidant capacity, large loss of enzyme activity, no bacteriostatic effect or weak bacteriostatic effect in the existing alcohol compound enzyme, and use high activity Food-grade enzyme preparations such as acid protease are used as the main raw materials, and scientifically compounded plant extracts, antioxidants, hop extracts, demycin, protective agents, activators, alcohol-resistant active dry yeast, etc., to prepare a comprehensive enzyme system, enzyme Alcohol compound enzyme containing acid protease with stable activity and high enzyme efficiency. This compound enzyme can effectively improve the yield and quality of alcohol, and is suitable for the preparation of alcohol or fuel ethanol from various raw materials

Method used

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  • Compound ethanol enzyme with acid proteases and method for preparing compound ethanol enzyme
  • Compound ethanol enzyme with acid proteases and method for preparing compound ethanol enzyme

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Experimental program
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Effect test

Embodiment 1

[0073] Embodiment 1 raw material preparation

[0074] 1. Preparation of plant extracts: uniformly mix barley malt and wheat malt in a mass ratio of 6:4, and grind them to a particle size of 0.8 mm to obtain crushed malt; then put papaya, pineapple, and fig in 0.4% sodium bicarbonate The solution was ultrasonically cleaned for 8 minutes in an ultrasonic cleaning machine with a power of 200W and a frequency of 30KHz, drained, crushed to a particle size of 0.8mm at room temperature, and uniformly mixed according to a mass ratio of 6:3:2, and crushed malt 3 times the mass of the mixture was added to obtain For the raw material mixture, add water twice the mass of the raw material mixture, adjust the pH value to 3.5 with citric acid, and carry out microwave extraction under the conditions of power 200W and frequency 2000Hz, wherein, the total time of each microwave irradiation is 70s, and the interval irradiation is carried out: Irradiate for 10s with an interval of 10s, control th...

Embodiment 2

[0101] An alcohol compound enzyme containing acidic protease is composed of the following raw materials in parts by weight:

[0102] 25 parts of amylase, 20 parts of protease, 12 parts of plant extract, 11 parts of hemicellulase, 11 parts of hop extract, 9 parts of pectinase, 9 parts of esterase, 9 parts of high temperature resistant dry yeast, demycin 8 parts, 6 parts of phytase, 5 parts of protective agent, 5 parts of activator, 0.9 part of antioxidant;

[0103] The amylase is uniformly mixed with an enzyme preparation composed of the following mass percentages: 70% glucoamylase, 10% pullulanase, 10% fungal α-amylase, 5% β-amylase, 5% medium temperature α- Amylase;

[0104] The protease is uniformly mixed with an enzyme preparation composed of the following mass percentages: 80% acid protease, 10% neutral protease, 10% proline protease;

[0105] The hemicellulase is uniformly mixed with an enzyme preparation composed of the following mass percentages: 35% heat-resistant β-...

Embodiment 3

[0114] An alcohol compound enzyme containing acidic protease is composed of the following raw materials in parts by weight:

[0115] 20 parts of amylase, 15 parts of protease, 10 parts of plant extract, 10 parts of hemicellulase, 10 parts of hop extract, 8 parts of pectinase, 8 parts of esterase, 7 parts of high temperature resistant dry yeast, demycin 5 parts, 5 parts of phytase, 4 parts of protective agent, 4 parts of activator, 0.6 part of antioxidant;

[0116] Described amylase, protease, hemicellulase, esterase mass composition are with embodiment 2;

[0117] The activator is formed by uniformly mixing inorganic salts with the following mass components: 15 parts of zinc chloride, 10 parts of calcium chloride, 10 parts of sodium sulfate, and 5 parts of magnesium chloride;

[0118] The protective agent is composed of the following raw materials in parts by weight: 20 parts of Ganoderma lucidum polysaccharide, 20 parts of trehalose, 10 parts of NaCl, (NH 4 ) 2 SO 4 10 pa...

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Abstract

The invention discloses a compound ethanol enzyme with acid proteases and a method for preparing the compound ethanol enzyme. The compound ethanol enzyme and the method have the advantages that the high-activity acid proteases and plant extract with abundant plant-based enzymes and nutrient substances are particularly compounded on the basis of scientific compound food-grade enzyme preparations, so that the compound ethanol enzyme is provided with complete enzyme systems; activators, protective agents and antioxidants are further added into the high-activity acid proteases and the plant extract, the enzyme activity can be completely released by the aid of the activators and can be stabilized by the aid of the protective agents and the antioxidants, and hops extract and novasil which are used for inhibiting bacteria contamination and promoting saccharification of leaven and quick proliferation and fermentation of saccharomyces cerevisiae are further scientifically compounded; starch, proteins, fat, cellulose, hemi-cellulose, pectin and the like in unprocessed grain can be sufficiently and effectively hydrolyzed, accordingly, the raw material utilization rate and the liquor yields can be effectively increased, and the alcohol contents can be increased by 13.63%, 36.25% and 17.44% respectively as compared with commercially available special compound ethanol enzymes; the liquor yields of raw materials can be respectively increased by 17.41%, 38.81% and 20.04%, and the ethanol production cost can be obviously reduced.

Description

technical field [0001] The invention relates to a compound enzyme, in particular to an alcohol compound enzyme containing acid protease and a preparation method thereof. Background technique [0002] The raw materials for producing alcohol are mainly food crops such as corn and wheat and non-food crops such as sweet potato (dry), cassava (dry), sugarcane molasses (orange water). In the fruits or rhizomes of these plants, in addition to a certain amount of starch polysaccharides, there are also some non-starch polysaccharides, pectin, protein, ash, vitamins and biotin. The high-temperature α-amylase and glucoamylase used in the liquefaction and saccharification of raw materials in alcohol factories can efficiently convert starch polysaccharides, but cannot hydrolyze non-starch polysaccharides, pectin, protein, ash and other substances. It depends on the endogenous enzymes secreted by the yeast itself for hydrolysis and utilization. However, due to environmental factors and ...

Claims

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Application Information

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IPC IPC(8): C12G3/02C12P7/06C12R1/865
CPCY02E50/10
Inventor 李洪兵李海清张锦杰朱永明胡永明易继云
Owner 湖南新鸿鹰生物工程有限公司
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