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Pestalotic acid compounds with antibacterial activity and applications thereof

A chemical compound, anti-bacterial technology, applied in the field of microbiology

Inactive Publication Date: 2019-08-13
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main means of controlling crop diseases, insect pests and weeds in the process of agricultural production is the control of chemical pesticides, which play a positive role in reducing diseases, insect pests and weeds, and ensuring high yields and harvests of crops. It is not recommended to rely too much on chemical pesticides for disease treatment; therefore, the research on plant endophytic fungi uses the symbiotic relationship between plant endophytic fungi and host plant biological activities to screen endophytic fungi with high biological activity and further obtain activity It is a hot spot of research in recent years

Method used

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  • Pestalotic acid compounds with antibacterial activity and applications thereof
  • Pestalotic acid compounds with antibacterial activity and applications thereof
  • Pestalotic acid compounds with antibacterial activity and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Separation and preparation of pestalotic acid compound:

[0065] a) Medium and culture conditions

[0066] Modified M-1-D Medium: NaH 2 PO 4 ·H 2 O 20mg, FeCl 3 2.0mg, MgSO 4 360mg, KCl 60mg, Ca(NO 3 )2 2280mg, KNO 3 80mg, 30g sucrose, 5g ammonium tartrate, 0.5g yeast extract, MnSO 4 5.0mg, ZnSO 4 ·7H 2 O2.5mg, H 3 BO 4 1.4mg, KI 0.7mg, distilled water 1000mL, agar 15-20g, pH natural.

[0067] Culture conditions: Strain cr014 was fermented with 30L of modified M-1-D medium and cultured at room temperature for 20 days.

[0068] b) Extraction and separation of fermentation products

[0069] The improved Fries medium that has been fermented for 20 days is cut into small pieces together with the bacterium colonies on it, and is soaked and extracted 3 times with ethyl acetate:methanol:acetic acid=80:15:5 (V / V / V) mixed solvent, and The three extracts were combined and concentrated, then extracted with ethyl acetate until the color did not change, and finall...

Embodiment 2

[0076] Pestalotic acid compound activity study:

[0077] a) Test compounds and reagents

[0078] Test compounds: compound 2, compound 3, compound 4, compound 5, compound 7, compound 8, compound 9;

[0079] Positive control: Cefotaxime;

[0080] Species tested: Ralstonia solanacearum, Salmonella typhi, Escherichia coli, Staphylococcus aureus.

[0081] b) Detection principle and method

[0082] The MICs of bacteria were determined by the modified microbial dilution method. Four strains of pathogenic bacteria were cultured on nutrient agar medium at 25°C for 18 to 24 hours, then the pathogenic bacterial cells were collected with a sterile inoculation loop, and cultured in a centrifuge tube filled with 10 mL of sterile normal saline. The concentration of the bacterial suspension was adjusted to 108 CFU / mL under standard conditions with an optical density of 0.10 at 630 nm. The bacterial suspension of this concentration was diluted 100 times before use, and the final concentra...

Embodiment 3

[0088] Pestalotic acid compound physical and chemical constants and spectral data:

[0089] Pestalotic acidA(1): brown oil, UV(MeOH)λmax(logε):292(4.10),206(4.25); NMR data see Tables 1 and 2; ESI-MS:349[M–H] - ; HR-ESI-MS: 349.1658 (349[M–H] - ,calc.349.1651).

[0090] Pestalotic acidB(2): brown oil, UV (MeOH)λ max (logε):318(4.16),232(4.30),207(4.40);ESI-MS:345[M-H] - ; HR-ESI-MS: 345.1338 ([M-H] - ,calc.345.1338).

[0091] Pestalotic acid C (3): brown oil, UV(MeOH)λ max (logε)nm:285(4.31),204(4.20);ESI-MS:357[M+H] + ,359[M+2+H] + ,379[M+Na] + ,381[M+2+Na] + ; HR-ESI-MS: 379.1284 ([M+Na] + ,calc.379.1288).

[0092] Pestalotic acidD (4): brown oil, UV(MeOH)λ max (logε)nm:317(3.82),217(4.52);ESI-MS:337[M-H] - ,339[M+2-H] - ; HR-EI-MS: 338.1285 ([M] + ,calc.338.1285).

[0093] Pestalotic acidE (5): brown oil, UV(MeOH)λ max (logε):310(3.47),208(4.07);ESI-MS:413[M+H] + ; HR-ESI-MS: 413.2665 ([M+H] + ,calc.413.2668).

[0094] Pestalotic acid F(6): bro...

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Abstract

The invention provides a Pestalotic acid compound with antibacterial activity. Hyperparasitism Pestalotiopsis neglecta strains are cultured in a fermented mode, extracted and separated to obtain the compound. The Pestalotic acid compound is easy to obtain, has high antibacterial activity and can be used as an antibacterial agent for preventing and controlling plant bacterial wilt, and a new path is provided for developing antibacterial agents and preventing and controlling plant diseases.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a class of Pestalotic acid compounds with antibacterial activity and applications thereof. Background technique [0002] Fungal endophyte refers to the fungi in the qualified organs of various tissues of living and healthy plants at a certain stage or all stages of the life cycle. They form a close symbiotic relationship with host plants through interactions, which can promote plant growth and provide plants with the ability to adapt to stress. Some endophytic fungi also have medicinal value. Finding and discovering active compounds from plant endophytic fungi has become another hot spot in domestic and foreign research. For example, Chinese patent document CN201310194836.5 discloses an endophytic fungus (Metarrhizium) LD421 isolated from Gentiana, which can be used to control dragon Bile leaf blight; patent application CN201210067829.4 discloses the endophytic fungus Fus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D303/38C07D307/79C07D493/04C07C59/90C07C59/56A01N43/90A01N37/42A01N37/38A01N43/20A01P1/00
CPCA01N37/38A01N37/42A01N43/20A01N43/90C07C59/56C07C59/90C07D303/38C07D307/79C07D493/04
Inventor 赵沛基李靖谢津
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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